Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses.
Identifieur interne : 004778 ( Main/Merge ); précédent : 004777; suivant : 004779Ubiquitin genes are differentially regulated in protoplast-derived cultures of Nicotiana sylvestris and in response to various stresses.
Auteurs : P. Genschik [France] ; Y. Parmentier ; A. Durr ; J. Marbach ; M C Criqui ; E. Jamet ; J. FleckSource :
- Plant molecular biology [ 0167-4412 ] ; 1992.
Descripteurs français
- KwdFr :
- ADN (génétique), ADN (isolement et purification), ARN (génétique), ARN (isolement et purification), ARN messager (biosynthèse), ARN messager (isolement et purification), Cartographie de restriction, Cellules cultivées, Données de séquences moléculaires, Famille multigénique, Humains, Masse moléculaire, Phénomènes physiologiques des plantes, Plantes (génétique), Protoplastes (physiologie), Régulation de l'expression des gènes, Similitude de séquences d'acides aminés, Similitude de séquences d'acides nucléiques, Sondes d'ADN, Séquence d'acides aminés, Séquence nucléotidique, Tabac (génétique), Tabac (physiologie), Ubiquitines (génétique), Végétaux toxiques.
- MESH :
- biosynthèse : ARN messager.
- génétique : ADN, ARN, Plantes, Tabac, Ubiquitines.
- isolement et purification : ADN, ARN, ARN messager.
- physiologie : Protoplastes, Tabac.
- Cartographie de restriction, Cellules cultivées, Données de séquences moléculaires, Famille multigénique, Humains, Masse moléculaire, Phénomènes physiologiques des plantes, Régulation de l'expression des gènes, Similitude de séquences d'acides aminés, Similitude de séquences d'acides nucléiques, Sondes d'ADN, Séquence d'acides aminés, Séquence nucléotidique, Végétaux toxiques.
English descriptors
- KwdEn :
- Amino Acid Sequence, Base Sequence, Cells, Cultured, DNA (genetics), DNA (isolation & purification), DNA Probes, Gene Expression Regulation, Humans, Molecular Sequence Data, Molecular Weight, Multigene Family, Plant Physiological Phenomena, Plants (genetics), Plants, Toxic, Protoplasts (physiology), RNA (genetics), RNA (isolation & purification), RNA, Messenger (biosynthesis), RNA, Messenger (isolation & purification), Restriction Mapping, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid, Tobacco (genetics), Tobacco (physiology), Ubiquitins (genetics).
- MESH :
- chemical , biosynthesis : RNA, Messenger.
- chemical , genetics : DNA, RNA, Ubiquitins.
- chemical , isolation & purification : DNA, RNA, RNA, Messenger.
- genetics : Plants, Tobacco.
- physiology : Protoplasts, Tobacco.
- Amino Acid Sequence, Base Sequence, Cells, Cultured, DNA Probes, Gene Expression Regulation, Humans, Molecular Sequence Data, Molecular Weight, Multigene Family, Plant Physiological Phenomena, Plants, Toxic, Restriction Mapping, Sequence Homology, Amino Acid, Sequence Homology, Nucleic Acid.
Abstract
Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5-mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.
DOI: 10.1007/bf00027161
PubMed: 1281439
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pubmed:1281439Le document en format XML
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Parmentier</name></author><author><name sortKey="Durr, A" sort="Durr, A" uniqKey="Durr A" first="A" last="Durr">A. Durr</name></author><author><name sortKey="Marbach, J" sort="Marbach, J" uniqKey="Marbach J" first="J" last="Marbach">J. Marbach</name></author><author><name sortKey="Criqui, M C" sort="Criqui, M C" uniqKey="Criqui M" first="M C" last="Criqui">M C Criqui</name></author><author><name sortKey="Jamet, E" sort="Jamet, E" uniqKey="Jamet E" first="E" last="Jamet">E. Jamet</name></author><author><name sortKey="Fleck, J" sort="Fleck, J" uniqKey="Fleck J" first="J" last="Fleck">J. 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Genschik</name><affiliation wicri:level="3"><nlm:affiliation>Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg, France.</nlm:affiliation><country xml:lang="fr">France</country><wicri:regionArea>Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg</wicri:regionArea><placeName><region type="region">Grand Est</region><region type="old region">Alsace (région administrative)</region><settlement type="city">Strasbourg</settlement></placeName></affiliation></author><author><name sortKey="Parmentier, Y" sort="Parmentier, Y" uniqKey="Parmentier Y" first="Y" last="Parmentier">Y. Parmentier</name></author><author><name sortKey="Durr, A" sort="Durr, A" uniqKey="Durr A" first="A" last="Durr">A. Durr</name></author><author><name sortKey="Marbach, J" sort="Marbach, J" uniqKey="Marbach J" first="J" last="Marbach">J. 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Fleck</name></author></analytic><series><title level="j">Plant molecular biology</title><idno type="ISSN">0167-4412</idno><imprint><date when="1992" type="published">1992</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cells, Cultured</term><term>DNA (genetics)</term><term>DNA (isolation & purification)</term><term>DNA Probes</term><term>Gene Expression Regulation</term><term>Humans</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Multigene Family</term><term>Plant Physiological Phenomena</term><term>Plants (genetics)</term><term>Plants, Toxic</term><term>Protoplasts (physiology)</term><term>RNA (genetics)</term><term>RNA (isolation & purification)</term><term>RNA, Messenger (biosynthesis)</term><term>RNA, Messenger (isolation & purification)</term><term>Restriction Mapping</term><term>Sequence Homology, Amino Acid</term><term>Sequence Homology, Nucleic Acid</term><term>Tobacco (genetics)</term><term>Tobacco (physiology)</term><term>Ubiquitins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN (génétique)</term><term>ADN (isolement et purification)</term><term>ARN (génétique)</term><term>ARN (isolement et purification)</term><term>ARN messager (biosynthèse)</term><term>ARN messager (isolement et purification)</term><term>Cartographie de restriction</term><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Famille multigénique</term><term>Humains</term><term>Masse moléculaire</term><term>Phénomènes physiologiques des plantes</term><term>Plantes (génétique)</term><term>Protoplastes (physiologie)</term><term>Régulation de l'expression des gènes</term><term>Similitude de séquences d'acides aminés</term><term>Similitude de séquences d'acides nucléiques</term><term>Sondes d'ADN</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Tabac (génétique)</term><term>Tabac (physiologie)</term><term>Ubiquitines (génétique)</term><term>Végétaux toxiques</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>RNA, Messenger</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term><term>RNA</term><term>Ubiquitins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA</term><term>RNA</term><term>RNA, Messenger</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plants</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term><term>ARN</term><term>Plantes</term><term>Tabac</term><term>Ubiquitines</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN</term><term>ARN</term><term>ARN messager</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Protoplastes</term><term>Tabac</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Protoplasts</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cells, Cultured</term><term>DNA Probes</term><term>Gene Expression Regulation</term><term>Humans</term><term>Molecular Sequence Data</term><term>Molecular Weight</term><term>Multigene Family</term><term>Plant Physiological Phenomena</term><term>Plants, Toxic</term><term>Restriction Mapping</term><term>Sequence Homology, Amino Acid</term><term>Sequence Homology, Nucleic Acid</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Cartographie de restriction</term><term>Cellules cultivées</term><term>Données de séquences moléculaires</term><term>Famille multigénique</term><term>Humains</term><term>Masse moléculaire</term><term>Phénomènes physiologiques des plantes</term><term>Régulation de l'expression des gènes</term><term>Similitude de séquences d'acides aminés</term><term>Similitude de séquences d'acides nucléiques</term><term>Sondes d'ADN</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Végétaux toxiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Four ubiquitin mRNA size classes were found to be differentially regulated in mesophyll protoplast-derived cultures of Nicotiana sylvestris. Three mRNA families of 1.9, 1.6 and 1.35 kb were expressed as soon as protoplasts were isolated. The 1.9 and 1.6 kb size classes were transiently expressed during the first hours of culture, whereas the level of expression of the 1.35 kb size class was maintained as long as cells kept dividing. A 0.7 kb mRNA size class started to be expressed just before the first divisions were observed. cDNAs corresponding to each of these families were isolated from a 6-h-old protoplast cDNA library and characterized. The 1.9, 1.6 and 1.35 kb mRNAs thus encode 7- or more, 6- and 5-mers, respectively, of ubiquitin whereas the 0.7 kb mRNAs encode a monomer of ubiquitin fused to a carboxyl extension protein of 52 amino acids. The expression of ubiquitin genes was studied, using probes specific for each of these transcript families, during protoplast culture and, for comparison, after various stresses including heat shock, HgCl2 treatment, a viral infection giving rise to a hypersensitive reaction, and an Agrobacterium tumefaciens infection which resulted in tumour formation. The 1.9 and 1.6 kb mRNA size classes were found to be stress-regulated, the 0.7 kb mRNA size class developmentally regulated and the 1.35 kb size class both stress- and developmentally regulated.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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