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In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium

Identifieur interne : 00AC46 ( Main/Exploration ); précédent : 00AC45; suivant : 00AC47

In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium

Auteurs : M. R. Chapman [États-Unis] ; G. W. Hutchinson ; M. J. Cenac ; T. R. Klei

Source :

(The) Journal of parasitology [ 0022-3395 ] ; 1994.

RBID : Pascal:94-0441433

Descripteurs français

Pascal (Inist)
- Parasite, Larve, Culture organisme, Milieu culture, Système acellulaire, Développement, Méthode, Strongylidae.

English descriptors

KwdEn :
- Cell free system, Culture medium, Development, Larva, Method, Organism culture, Parasite.

Abstract

An efficient and reliable method is described for the culture of equine strongyles from the third (L₃) to the fourth (L₄) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO₃, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO₂, 5% O₂, and 85% N₂. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L₄ stage

Affiliations:

États-Unis

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000951
to stream PascalFrancis, to step Curation: 000935
to stream PascalFrancis, to step Checkpoint: 000930
to stream Main, to step Merge: 00B290
to stream Main, to step Curation: 00AC46

Le document en format XML

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<author><name sortKey="Chapman, M R" sort="Chapman, M R" uniqKey="Chapman M" first="M. R." last="Chapman">M. R. Chapman</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>LSU agricultural cent., Louisiana agricultural exp. stn., dep. veterinary sci.</s1>
<s2>Baton Rouge LA 70803</s2>
<s3>USA</s3>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Baton Rouge LA 70803</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hutchinson, G W" sort="Hutchinson, G W" uniqKey="Hutchinson G" first="G. W." last="Hutchinson">G. W. Hutchinson</name>
</author>
<author><name sortKey="Cenac, M J" sort="Cenac, M J" uniqKey="Cenac M" first="M. J." last="Cenac">M. J. Cenac</name>
</author>
<author><name sortKey="Klei, T R" sort="Klei, T R" uniqKey="Klei T" first="T. R." last="Klei">T. R. Klei</name>
</author>
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<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">94-0441433</idno>
<date when="1994">1994</date>
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<idno type="RBID">Pascal:94-0441433</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000951</idno>
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<idno type="wicri:doubleKey">0022-3395:1994:Chapman M:in:vitro:culture</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium</title>
<author><name sortKey="Chapman, M R" sort="Chapman, M R" uniqKey="Chapman M" first="M. R." last="Chapman">M. R. Chapman</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>LSU agricultural cent., Louisiana agricultural exp. stn., dep. veterinary sci.</s1>
<s2>Baton Rouge LA 70803</s2>
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<country>États-Unis</country>
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<author><name sortKey="Hutchinson, G W" sort="Hutchinson, G W" uniqKey="Hutchinson G" first="G. W." last="Hutchinson">G. W. Hutchinson</name>
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<author><name sortKey="Cenac, M J" sort="Cenac, M J" uniqKey="Cenac M" first="M. J." last="Cenac">M. J. Cenac</name>
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<author><name sortKey="Klei, T R" sort="Klei, T R" uniqKey="Klei T" first="T. R." last="Klei">T. R. Klei</name>
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<series><title level="j" type="main">(The) Journal of parasitology</title>
<title level="j" type="abbreviated">J. parasitol.</title>
<idno type="ISSN">0022-3395</idno>
<imprint><date when="1994">1994</date>
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<seriesStmt><title level="j" type="main">(The) Journal of parasitology</title>
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<idno type="ISSN">0022-3395</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell free system</term>
<term>Culture medium</term>
<term>Development</term>
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<term>Method</term>
<term>Organism culture</term>
<term>Parasite</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Parasite</term>
<term>Larve</term>
<term>Culture organisme</term>
<term>Milieu culture</term>
<term>Système acellulaire</term>
<term>Développement</term>
<term>Méthode</term>
<term>Strongylidae</term>
</keywords>
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</teiHeader>
<front><div type="abstract" xml:lang="en">An efficient and reliable method is described for the culture of equine strongyles from the third (L<sub>3</sub>
) to the fourth (L<sub>4</sub>
) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO<sub>3</sub>
, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO<sub>2</sub>
, 5% O<sub>2</sub>
, and 85% N<sub>2</sub>
. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L<sub>4</sub>
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<affiliations><list><country><li>États-Unis</li>
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<tree><noCountry><name sortKey="Cenac, M J" sort="Cenac, M J" uniqKey="Cenac M" first="M. J." last="Cenac">M. J. Cenac</name>
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<name sortKey="Klei, T R" sort="Klei, T R" uniqKey="Klei T" first="T. R." last="Klei">T. R. Klei</name>
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<country name="États-Unis"><noRegion><name sortKey="Chapman, M R" sort="Chapman, M R" uniqKey="Chapman M" first="M. R." last="Chapman">M. R. Chapman</name>
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In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium

In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri