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In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium

Identifieur interne : 000951 ( PascalFrancis/Corpus ); précédent : 000950; suivant : 000952

In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium

Auteurs : M. R. Chapman ; G. W. Hutchinson ; M. J. Cenac ; T. R. Klei

Source :

RBID : Pascal:94-0441433

Descripteurs français

English descriptors

Abstract

An efficient and reliable method is described for the culture of equine strongyles from the third (L3) to the fourth (L4) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO3, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO2, 5% O2, and 85% N2. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L4 stage

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 0022-3395
A02 01      @0 JOPAA2
A03   1    @0 J. parasitol.
A05       @2 80
A06       @2 2
A08 01  1  ENG  @1 In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium
A11 01  1    @1 CHAPMAN (M. R.)
A11 02  1    @1 HUTCHINSON (G. W.)
A11 03  1    @1 CENAC (M. J.)
A11 04  1    @1 KLEI (T. R.)
A14 01      @1 LSU agricultural cent., Louisiana agricultural exp. stn., dep. veterinary sci. @2 Baton Rouge LA 70803 @3 USA
A20       @1 225-231
A21       @1 1994
A23 01      @0 ENG
A43 01      @1 INIST @2 2087 @5 354000045392360020
A44       @0 0000
A45       @0 19 ref.
A47 01  1    @0 94-0441433
A60       @1 P
A61       @0 A
A64 01  1    @0 (The) Journal of parasitology
A66 01      @0 USA
C01 01    ENG  @0 An efficient and reliable method is described for the culture of equine strongyles from the third (L3) to the fourth (L4) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO3, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO2, 5% O2, and 85% N2. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L4 stage
C02 01  X    @0 002A12A02B
C02 02  X    @0 260
C03 01  X  FRE  @0 Parasite @5 01
C03 01  X  ENG  @0 Parasite @5 01
C03 01  X  SPA  @0 Parásito @5 01
C03 02  X  FRE  @0 Larve @5 02
C03 02  X  ENG  @0 Larva @5 02
C03 02  X  SPA  @0 Larva @5 02
C03 03  X  FRE  @0 Culture organisme @5 03
C03 03  X  ENG  @0 Organism culture @5 03
C03 03  X  SPA  @0 Cultivo organismo @5 03
C03 04  X  FRE  @0 Milieu culture @5 04
C03 04  X  ENG  @0 Culture medium @5 04
C03 04  X  SPA  @0 Medio cultivo @5 04
C03 05  X  FRE  @0 Système acellulaire @5 05
C03 05  X  ENG  @0 Cell free system @5 05
C03 05  X  SPA  @0 Sistema acelular @5 05
C03 06  X  FRE  @0 Développement @5 06
C03 06  X  ENG  @0 Development @5 06
C03 06  X  GER  @0 Entwicklung @5 06
C03 06  X  SPA  @0 Desarrollo @5 06
C03 07  X  FRE  @0 Méthode @5 07
C03 07  X  ENG  @0 Method @5 07
C03 07  X  SPA  @0 Método @5 07
C03 08  X  FRE  @0 Strongylidae @2 NS @4 INC @5 66
C07 01  X  FRE  @0 Nematoda @2 NS @5 60
C07 01  X  ENG  @0 Nematoda @2 NS @5 60
C07 01  X  SPA  @0 Nematoda @2 NS @5 60
C07 02  X  FRE  @0 Nemathelminthia @2 NS
C07 02  X  ENG  @0 Nemathelminthia @2 NS
C07 02  X  SPA  @0 Nemathelminthia @2 NS
C07 03  X  FRE  @0 Helmintha @2 NS
C07 03  X  ENG  @0 Helmintha @2 NS
C07 03  X  SPA  @0 Helmintha @2 NS
C07 04  X  FRE  @0 Invertebrata @2 NS
C07 04  X  ENG  @0 Invertebrata @2 NS
C07 04  X  SPA  @0 Invertebrata @2 NS
C07 05  X  FRE  @0 Perissodactyla @2 NS @5 61
C07 05  X  ENG  @0 Perissodactyla @2 NS @5 61
C07 05  X  SPA  @0 Perissodactyla @2 NS @5 61
C07 06  X  FRE  @0 Ungulata @2 NS
C07 06  X  ENG  @0 Ungulata @2 NS
C07 06  X  SPA  @0 Ungulata @2 NS
C07 07  X  FRE  @0 Mammalia @2 NS
C07 07  X  ENG  @0 Mammalia @2 NS
C07 07  X  SPA  @0 Mammalia @2 NS
C07 08  X  FRE  @0 Vertebrata @2 NS
C07 08  X  ENG  @0 Vertebrata @2 NS
C07 08  X  SPA  @0 Vertebrata @2 NS
N21       @1 201

Format Inist (serveur)

NO : PASCAL 94-0441433 INIST
ET : In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium
AU : CHAPMAN (M. R.); HUTCHINSON (G. W.); CENAC (M. J.); KLEI (T. R.)
AF : LSU agricultural cent., Louisiana agricultural exp. stn., dep. veterinary sci./Baton Rouge LA 70803/Etats-Unis
DT : Publication en série; Niveau analytique
SO : (The) Journal of parasitology; ISSN 0022-3395; Coden JOPAA2; Etats-Unis; Da. 1994; Vol. 80; No. 2; Pp. 225-231; Bibl. 19 ref.
LA : Anglais
EA : An efficient and reliable method is described for the culture of equine strongyles from the third (L3) to the fourth (L4) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO3, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO2, 5% O2, and 85% N2. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L4 stage
CC : 002A12A02B; 260
FD : Parasite; Larve; Culture organisme; Milieu culture; Système acellulaire; Développement; Méthode; Strongylidae
FG : Nematoda; Nemathelminthia; Helmintha; Invertebrata; Perissodactyla; Ungulata; Mammalia; Vertebrata
ED : Parasite; Larva; Organism culture; Culture medium; Cell free system; Development; Method
EG : Nematoda; Nemathelminthia; Helmintha; Invertebrata; Perissodactyla; Ungulata; Mammalia; Vertebrata
GD : Entwicklung
SD : Parásito; Larva; Cultivo organismo; Medio cultivo; Sistema acelular; Desarrollo; Método
LO : INIST-2087.354000045392360020
ID : 94-0441433

Links to Exploration step

Pascal:94-0441433

Le document en format XML

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<s1>201</s1>
</fN21>
</pA>
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<server>
<NO>PASCAL 94-0441433 INIST</NO>
<ET>In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium</ET>
<AU>CHAPMAN (M. R.); HUTCHINSON (G. W.); CENAC (M. J.); KLEI (T. R.)</AU>
<AF>LSU agricultural cent., Louisiana agricultural exp. stn., dep. veterinary sci./Baton Rouge LA 70803/Etats-Unis</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>(The) Journal of parasitology; ISSN 0022-3395; Coden JOPAA2; Etats-Unis; Da. 1994; Vol. 80; No. 2; Pp. 225-231; Bibl. 19 ref.</SO>
<LA>Anglais</LA>
<EA>An efficient and reliable method is described for the culture of equine strongyles from the third (L
<sub>3</sub>
) to the fourth (L
<sub>4</sub>
) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO
<sub>3</sub>
, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO
<sub>2</sub>
, 5% O
<sub>2</sub>
, and 85% N
<sub>2</sub>
. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L
<sub>4</sub>
stage</EA>
<CC>002A12A02B; 260</CC>
<FD>Parasite; Larve; Culture organisme; Milieu culture; Système acellulaire; Développement; Méthode; Strongylidae</FD>
<FG>Nematoda; Nemathelminthia; Helmintha; Invertebrata; Perissodactyla; Ungulata; Mammalia; Vertebrata</FG>
<ED>Parasite; Larva; Organism culture; Culture medium; Cell free system; Development; Method</ED>
<EG>Nematoda; Nemathelminthia; Helmintha; Invertebrata; Perissodactyla; Ungulata; Mammalia; Vertebrata</EG>
<GD>Entwicklung</GD>
<SD>Parásito; Larva; Cultivo organismo; Medio cultivo; Sistema acelular; Desarrollo; Método</SD>
<LO>INIST-2087.354000045392360020</LO>
<ID>94-0441433</ID>
</server>
</inist>
</record>

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