In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium
Identifieur interne : 00AC46 ( Main/Curation ); précédent : 00AC45; suivant : 00AC47In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium
Auteurs : M. R. Chapman [États-Unis] ; G. W. Hutchinson ; M. J. Cenac ; T. R. KleiSource :
- (The) Journal of parasitology [ 0022-3395 ] ; 1994.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
An efficient and reliable method is described for the culture of equine strongyles from the third (L3) to the fourth (L4) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO3, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO2, 5% O2, and 85% N2. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L4 stage
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Pascal:94-0441433Le document en format XML
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<author><name sortKey="Chapman, M R" sort="Chapman, M R" uniqKey="Chapman M" first="M. R." last="Chapman">M. R. Chapman</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>LSU agricultural cent., Louisiana agricultural exp. stn., dep. veterinary sci.</s1>
<s2>Baton Rouge LA 70803</s2>
<s3>USA</s3>
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<country>États-Unis</country>
<wicri:noRegion>Baton Rouge LA 70803</wicri:noRegion>
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<author><name sortKey="Hutchinson, G W" sort="Hutchinson, G W" uniqKey="Hutchinson G" first="G. W." last="Hutchinson">G. W. Hutchinson</name>
</author>
<author><name sortKey="Cenac, M J" sort="Cenac, M J" uniqKey="Cenac M" first="M. J." last="Cenac">M. J. Cenac</name>
</author>
<author><name sortKey="Klei, T R" sort="Klei, T R" uniqKey="Klei T" first="T. R." last="Klei">T. R. Klei</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium</title>
<author><name sortKey="Chapman, M R" sort="Chapman, M R" uniqKey="Chapman M" first="M. R." last="Chapman">M. R. Chapman</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>LSU agricultural cent., Louisiana agricultural exp. stn., dep. veterinary sci.</s1>
<s2>Baton Rouge LA 70803</s2>
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<country>États-Unis</country>
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<author><name sortKey="Hutchinson, G W" sort="Hutchinson, G W" uniqKey="Hutchinson G" first="G. W." last="Hutchinson">G. W. Hutchinson</name>
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<author><name sortKey="Cenac, M J" sort="Cenac, M J" uniqKey="Cenac M" first="M. J." last="Cenac">M. J. Cenac</name>
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<author><name sortKey="Klei, T R" sort="Klei, T R" uniqKey="Klei T" first="T. R." last="Klei">T. R. Klei</name>
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<series><title level="j" type="main">(The) Journal of parasitology</title>
<title level="j" type="abbreviated">J. parasitol.</title>
<idno type="ISSN">0022-3395</idno>
<imprint><date when="1994">1994</date>
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<seriesStmt><title level="j" type="main">(The) Journal of parasitology</title>
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<idno type="ISSN">0022-3395</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell free system</term>
<term>Culture medium</term>
<term>Development</term>
<term>Larva</term>
<term>Method</term>
<term>Organism culture</term>
<term>Parasite</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Parasite</term>
<term>Larve</term>
<term>Culture organisme</term>
<term>Milieu culture</term>
<term>Système acellulaire</term>
<term>Développement</term>
<term>Méthode</term>
<term>Strongylidae</term>
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<front><div type="abstract" xml:lang="en">An efficient and reliable method is described for the culture of equine strongyles from the third (L<sub>3</sub>
) to the fourth (L<sub>4</sub>
) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO<sub>3</sub>
, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO<sub>2</sub>
, 5% O<sub>2</sub>
, and 85% N<sub>2</sub>
. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L<sub>4</sub>
stage</div>
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