In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium
Identifieur interne : 00B290 ( Main/Merge ); précédent : 00B289; suivant : 00B291In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium
Auteurs : M. R. Chapman [États-Unis] ; G. W. Hutchinson ; M. J. Cenac ; T. R. KleiSource :
- (The) Journal of parasitology [ 0022-3395 ] ; 1994.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
An efficient and reliable method is described for the culture of equine strongyles from the third (L3) to the fourth (L4) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO3, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO2, 5% O2, and 85% N2. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L4 stage
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000951
- to stream PascalFrancis, to step Curation: 000935
- to stream PascalFrancis, to step Checkpoint: 000930
Links to Exploration step
Pascal:94-0441433Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium</title>
<author><name sortKey="Chapman, M R" sort="Chapman, M R" uniqKey="Chapman M" first="M. R." last="Chapman">M. R. Chapman</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>LSU agricultural cent., Louisiana agricultural exp. stn., dep. veterinary sci.</s1>
<s2>Baton Rouge LA 70803</s2>
<s3>USA</s3>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Baton Rouge LA 70803</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hutchinson, G W" sort="Hutchinson, G W" uniqKey="Hutchinson G" first="G. W." last="Hutchinson">G. W. Hutchinson</name>
</author>
<author><name sortKey="Cenac, M J" sort="Cenac, M J" uniqKey="Cenac M" first="M. J." last="Cenac">M. J. Cenac</name>
</author>
<author><name sortKey="Klei, T R" sort="Klei, T R" uniqKey="Klei T" first="T. R." last="Klei">T. R. Klei</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">94-0441433</idno>
<date when="1994">1994</date>
<idno type="stanalyst">PASCAL 94-0441433 INIST</idno>
<idno type="RBID">Pascal:94-0441433</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000951</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000935</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000930</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">000930</idno>
<idno type="wicri:doubleKey">0022-3395:1994:Chapman M:in:vitro:culture</idno>
<idno type="wicri:Area/Main/Merge">00B290</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium</title>
<author><name sortKey="Chapman, M R" sort="Chapman, M R" uniqKey="Chapman M" first="M. R." last="Chapman">M. R. Chapman</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>LSU agricultural cent., Louisiana agricultural exp. stn., dep. veterinary sci.</s1>
<s2>Baton Rouge LA 70803</s2>
<s3>USA</s3>
</inist:fA14>
<country>États-Unis</country>
<wicri:noRegion>Baton Rouge LA 70803</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hutchinson, G W" sort="Hutchinson, G W" uniqKey="Hutchinson G" first="G. W." last="Hutchinson">G. W. Hutchinson</name>
</author>
<author><name sortKey="Cenac, M J" sort="Cenac, M J" uniqKey="Cenac M" first="M. J." last="Cenac">M. J. Cenac</name>
</author>
<author><name sortKey="Klei, T R" sort="Klei, T R" uniqKey="Klei T" first="T. R." last="Klei">T. R. Klei</name>
</author>
</analytic>
<series><title level="j" type="main">(The) Journal of parasitology</title>
<title level="j" type="abbreviated">J. parasitol.</title>
<idno type="ISSN">0022-3395</idno>
<imprint><date when="1994">1994</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">(The) Journal of parasitology</title>
<title level="j" type="abbreviated">J. parasitol.</title>
<idno type="ISSN">0022-3395</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cell free system</term>
<term>Culture medium</term>
<term>Development</term>
<term>Larva</term>
<term>Method</term>
<term>Organism culture</term>
<term>Parasite</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Parasite</term>
<term>Larve</term>
<term>Culture organisme</term>
<term>Milieu culture</term>
<term>Système acellulaire</term>
<term>Développement</term>
<term>Méthode</term>
<term>Strongylidae</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">An efficient and reliable method is described for the culture of equine strongyles from the third (L<sub>3</sub>
) to the fourth (L<sub>4</sub>
) larval stage. Medium consists of 50% fetal calf serum and 50% NCTC with additions of L-glutamine, NaHCO<sub>3</sub>
, yeast extract, bactopeptone, and dextrose. The gas phase used is of prime importance; it is a mixture of 10% CO<sub>2</sub>
, 5% O<sub>2</sub>
, and 85% N<sub>2</sub>
. Strongylus vulgaris, Strongylus edentatus, Strongylus equinus Triodontophorus brevicauda, Triodontophorus serratus, Triodontophorus tenuicolis, Oesophagodontus robustus, Cylicocyclus insigne, and mixed species of cyathostomes were cultured to the L<sub>4</sub>
stage</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
</list>
<tree><noCountry><name sortKey="Cenac, M J" sort="Cenac, M J" uniqKey="Cenac M" first="M. J." last="Cenac">M. J. Cenac</name>
<name sortKey="Hutchinson, G W" sort="Hutchinson, G W" uniqKey="Hutchinson G" first="G. W." last="Hutchinson">G. W. Hutchinson</name>
<name sortKey="Klei, T R" sort="Klei, T R" uniqKey="Klei T" first="T. R." last="Klei">T. R. Klei</name>
</noCountry>
<country name="États-Unis"><noRegion><name sortKey="Chapman, M R" sort="Chapman, M R" uniqKey="Chapman M" first="M. R." last="Chapman">M. R. Chapman</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Santé/explor/EdenteV2/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 00B290 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 00B290 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Santé |area= EdenteV2 |flux= Main |étape= Merge |type= RBID |clé= Pascal:94-0441433 |texte= In vitro culture of equine strongylidae to the fourth larval stage in a cell-free medium }}
This area was generated with Dilib version V0.6.32. |