Anticancer activities of chalcone flavokawain B from Alpinia pricei Hayata in human lung adenocarcinoma (A549) cells via induction of reactive oxygen species-mediated apoptotic and autophagic cell death.
Identifieur interne : 000A76 ( Main/Exploration ); précédent : 000A75; suivant : 000A77Anticancer activities of chalcone flavokawain B from Alpinia pricei Hayata in human lung adenocarcinoma (A549) cells via induction of reactive oxygen species-mediated apoptotic and autophagic cell death.
Auteurs : You-Cheng Hseu [Taïwan] ; Yu-Chi Huang [Taïwan] ; Varadharajan Thiyagarajan [Taïwan] ; Dony Chacko Mathew [Taïwan] ; Kai-Yuan Lin [Taïwan] ; Ssu-Ching Chen [Taïwan] ; Jer-Yuh Liu [Taïwan] ; Li-Sung Hsu [Taïwan] ; Mei-Ling Li [Taïwan] ; Hsin-Ling Yang [Taïwan]Source :
- Journal of cellular physiology [ 1097-4652 ] ; 2019.
Abstract
Chalcones found in fruits and vegetables have promising cancer chemopreventive properties. This study attempts to identify the anticancer efficacies of chalcone flavokawain B (FKB) in the rhizomes of Alpinia pricei Hayata by examining key molecular events in non-small-cell lung cancer (A549) cells. Our results indicated that in human A549 cells, FKB (0-15 μg/ml) decreases cell viability and colony formation, dysregulates the Bax:B-cell lymphoma 2 ratio and increases apoptotic DNA fragmentation. Mitochondrial (caspase-9/-3 and poly ADP ribose polymerase [PARP]) signaling was found to be involved in FKB-induced apoptosis. In addition, FKB-induced reactive oxygen species (ROS) generation, and N-acetylcysteine attenuated FKB-induced apoptotic cell death. Moreover, FKB triggered autophagy, as evidenced by the improved acidic vesicular organelle formation, lipidated light chain 3 (microtubule-related light chain 3) accumulation, and ATG7 expression and the decreased mammalian target of rapamycin phosphorylation. Furthermore, FKB suppressed ROS-mediated ATG4B expression. Inhibiting autophagy using 3-methyladenine/chloroquine diminished FKB-induced cell death, indicating that autophagy is triggered as a death mechanism by FKB. In summary, FKB has a crucial role in the execution and propagation of ROS-mediated apoptotic and autophagic cell death of lung adenocarcinoma cells.
DOI: 10.1002/jcp.28375
PubMed: 30847898
Affiliations:
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oxygen species-mediated apoptotic and autophagic cell death.</title><author><name sortKey="Hseu, You Cheng" sort="Hseu, You Cheng" uniqKey="Hseu Y" first="You-Cheng" last="Hseu">You-Cheng Hseu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Yu Chi" sort="Huang, Yu Chi" uniqKey="Huang Y" first="Yu-Chi" last="Huang">Yu-Chi Huang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation></author><author><name sortKey="Thiyagarajan, Varadharajan" sort="Thiyagarajan, Varadharajan" uniqKey="Thiyagarajan V" first="Varadharajan" last="Thiyagarajan">Varadharajan Thiyagarajan</name><affiliation wicri:level="1"><nlm:affiliation>Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation></author><author><name sortKey="Mathew, Dony Chacko" sort="Mathew, Dony Chacko" uniqKey="Mathew D" first="Dony Chacko" last="Mathew">Dony Chacko Mathew</name><affiliation wicri:level="1"><nlm:affiliation>Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Cosmeceutics, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Kai Yuan" sort="Lin, Kai Yuan" uniqKey="Lin K" first="Kai-Yuan" last="Lin">Kai-Yuan Lin</name><affiliation wicri:level="1"><nlm:affiliation>Department of Medical Research, Chi-Mei Medical Center, Tainan, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Medical Research, Chi-Mei Medical Center, Tainan</wicri:regionArea><wicri:noRegion>Tainan</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Ssu Ching" sort="Chen, Ssu Ching" uniqKey="Chen S" first="Ssu-Ching" last="Chen">Ssu-Ching Chen</name><affiliation wicri:level="1"><nlm:affiliation>Department of Life Sciences, National Central University, Chung-Li, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Life Sciences, National Central University, Chung-Li</wicri:regionArea><wicri:noRegion>Chung-Li</wicri:noRegion></affiliation></author><author><name sortKey="Liu, Jer Yuh" sort="Liu, Jer Yuh" uniqKey="Liu J" first="Jer-Yuh" last="Liu">Jer-Yuh Liu</name><affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Cancer Biology, China Medical University, Taichung, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Graduate Institute of Cancer Biology, China Medical University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation></author><author><name sortKey="Hsu, Li Sung" sort="Hsu, Li Sung" uniqKey="Hsu L" first="Li-Sung" last="Hsu">Li-Sung Hsu</name><affiliation wicri:level="1"><nlm:affiliation>Department of Biomedical Sciences, Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Biomedical Sciences, Institute of Biochemistry and Biotechnology, Chung Shan Medical University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation></author><author><name sortKey="Li, Mei Ling" sort="Li, Mei Ling" uniqKey="Li M" first="Mei-Ling" last="Li">Mei-Ling Li</name><affiliation wicri:level="1"><nlm:affiliation>Department of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation></author><author><name sortKey="Yang, Hsin Ling" sort="Yang, Hsin Ling" uniqKey="Yang H" first="Hsin-Ling" last="Yang">Hsin-Ling Yang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Nutrition, College of Biopharmaceutical and Food Sciences, China Medical University, Taichung</wicri:regionArea><wicri:noRegion>Taichung</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of cellular physiology</title><idno type="eISSN">1097-4652</idno><imprint><date when="2019" type="published">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Chalcones found in fruits and vegetables have promising cancer chemopreventive properties. This study attempts to identify the anticancer efficacies of chalcone flavokawain B (FKB) in the rhizomes of Alpinia pricei Hayata by examining key molecular events in non-small-cell lung cancer (A549) cells. Our results indicated that in human A549 cells, FKB (0-15 μg/ml) decreases cell viability and colony formation, dysregulates the Bax:B-cell lymphoma 2 ratio and increases apoptotic DNA fragmentation. Mitochondrial (caspase-9/-3 and poly ADP ribose polymerase [PARP]) signaling was found to be involved in FKB-induced apoptosis. In addition, FKB-induced reactive oxygen species (ROS) generation, and N-acetylcysteine attenuated FKB-induced apoptotic cell death. Moreover, FKB triggered autophagy, as evidenced by the improved acidic vesicular organelle formation, lipidated light chain 3 (microtubule-related light chain 3) accumulation, and ATG7 expression and the decreased mammalian target of rapamycin phosphorylation. Furthermore, FKB suppressed ROS-mediated ATG4B expression. Inhibiting autophagy using 3-methyladenine/chloroquine diminished FKB-induced cell death, indicating that autophagy is triggered as a death mechanism by FKB. In summary, FKB has a crucial role in the execution and propagation of ROS-mediated apoptotic and autophagic cell death of lung adenocarcinoma cells.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Hseu, You Cheng" sort="Hseu, You Cheng" uniqKey="Hseu Y" first="You-Cheng" last="Hseu">You-Cheng Hseu</name></noRegion><name sortKey="Chen, Ssu Ching" sort="Chen, Ssu Ching" uniqKey="Chen S" first="Ssu-Ching" last="Chen">Ssu-Ching Chen</name><name sortKey="Hsu, Li Sung" sort="Hsu, Li Sung" uniqKey="Hsu L" first="Li-Sung" last="Hsu">Li-Sung Hsu</name><name sortKey="Huang, Yu Chi" sort="Huang, Yu Chi" uniqKey="Huang Y" first="Yu-Chi" last="Huang">Yu-Chi Huang</name><name sortKey="Li, Mei Ling" sort="Li, Mei Ling" uniqKey="Li M" first="Mei-Ling" last="Li">Mei-Ling Li</name><name sortKey="Lin, Kai Yuan" sort="Lin, Kai Yuan" uniqKey="Lin K" first="Kai-Yuan" last="Lin">Kai-Yuan Lin</name><name sortKey="Liu, Jer Yuh" sort="Liu, Jer Yuh" uniqKey="Liu J" first="Jer-Yuh" last="Liu">Jer-Yuh Liu</name><name sortKey="Mathew, Dony Chacko" sort="Mathew, Dony Chacko" uniqKey="Mathew D" first="Dony Chacko" last="Mathew">Dony Chacko Mathew</name><name sortKey="Thiyagarajan, Varadharajan" sort="Thiyagarajan, Varadharajan" uniqKey="Thiyagarajan V" first="Varadharajan" last="Thiyagarajan">Varadharajan Thiyagarajan</name><name sortKey="Yang, Hsin Ling" sort="Yang, Hsin Ling" uniqKey="Yang H" first="Hsin-Ling" last="Yang">Hsin-Ling Yang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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