A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method
Identifieur interne : 000A06 ( Main/Merge ); précédent : 000A05; suivant : 000A07A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method
Auteurs : D. W. Baker [Canada] ; C. May [Canada] ; C. J. Brauner [Canada]Source :
- Journal of Fish Biology [ 0022-1112 ] ; 2009-07.
English descriptors
- KwdEn :
- Acidosis (blood), Acidosis (etiology), Acidosis (pathology), Acidosis (veterinary), Acipenser transmontanus, Animals, CO2, Carbon Dioxide (analysis), Carbon Dioxide (pharmacology), Erythrocytes (chemistry), Erythrocytes (drug effects), Fish Diseases (blood), Fish Diseases (etiology), Fish Diseases (pathology), Fishes (physiology), Freezing, Hydrogen-Ion Concentration, Intracellular Fluid (chemistry), Reproducibility of Results, Specimen Handling (methods), Specimen Handling (standards), Time Factors, Zoology (methods), acid‐base balance, hypercapnia, hypercarbia, tissue pH.
- MESH :
- chemical , analysis : Carbon Dioxide.
- blood : Acidosis, Fish Diseases.
- chemistry : Erythrocytes, Intracellular Fluid.
- drug effects : Erythrocytes.
- etiology : Acidosis, Fish Diseases.
- methods : Specimen Handling, Zoology.
- pathology : Acidosis, Fish Diseases.
- chemical , pharmacology : Carbon Dioxide.
- physiology : Fishes.
- standards : Specimen Handling.
- veterinary : Acidosis.
- Animals, Freezing, Hydrogen-Ion Concentration, Reproducibility of Results, Time Factors.
Abstract
This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).
Url:
DOI: 10.1111/j.1095-8649.2009.02261.x
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No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).</div></front></TEI></PubMed></double></record>Pour manipuler ce document sous Unix (Dilib)
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