A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method.
Identifieur interne : 000345 ( Ncbi/Merge ); précédent : 000344; suivant : 000346A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method.
Auteurs : D W Baker [Canada] ; C. May ; C J BraunerSource :
- Journal of fish biology [ 1095-8649 ] ; 2009.
English descriptors
- KwdEn :
- Acidosis (blood), Acidosis (etiology), Acidosis (pathology), Acidosis (veterinary), Animals, Carbon Dioxide (analysis), Carbon Dioxide (pharmacology), Erythrocytes (chemistry), Erythrocytes (drug effects), Fish Diseases (blood), Fish Diseases (etiology), Fish Diseases (pathology), Fishes (physiology), Freezing, Hydrogen-Ion Concentration, Intracellular Fluid (chemistry), Reproducibility of Results, Specimen Handling (methods), Specimen Handling (standards), Time Factors, Zoology (methods).
- MESH :
- chemical , analysis : Carbon Dioxide.
- blood : Acidosis, Fish Diseases.
- chemistry : Erythrocytes, Intracellular Fluid.
- drug effects : Erythrocytes.
- etiology : Acidosis, Fish Diseases.
- methods : Specimen Handling, Zoology.
- pathology : Acidosis, Fish Diseases.
- chemical , pharmacology : Carbon Dioxide.
- physiology : Fishes.
- standards : Specimen Handling.
- veterinary : Acidosis.
- Animals, Freezing, Hydrogen-Ion Concentration, Reproducibility of Results, Time Factors.
Abstract
This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).
DOI: 10.1111/j.1095-8649.2009.02261.x
PubMed: 20738495
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pubmed:20738495Le document en format XML
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May</name></author><author><name sortKey="Brauner, C J" sort="Brauner, C J" uniqKey="Brauner C" first="C J" last="Brauner">C J Brauner</name></author></analytic><series><title level="j">Journal of fish biology</title><idno type="eISSN">1095-8649</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Acidosis (blood)</term><term>Acidosis (etiology)</term><term>Acidosis (pathology)</term><term>Acidosis (veterinary)</term><term>Animals</term><term>Carbon Dioxide (analysis)</term><term>Carbon Dioxide (pharmacology)</term><term>Erythrocytes (chemistry)</term><term>Erythrocytes (drug effects)</term><term>Fish Diseases (blood)</term><term>Fish Diseases (etiology)</term><term>Fish Diseases (pathology)</term><term>Fishes (physiology)</term><term>Freezing</term><term>Hydrogen-Ion Concentration</term><term>Intracellular Fluid (chemistry)</term><term>Reproducibility of Results</term><term>Specimen Handling (methods)</term><term>Specimen Handling (standards)</term><term>Time Factors</term><term>Zoology (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Carbon Dioxide</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Acidosis</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Erythrocytes</term><term>Intracellular Fluid</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Erythrocytes</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Acidosis</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Specimen Handling</term><term>Zoology</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Acidosis</term><term>Fish Diseases</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Carbon Dioxide</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Fishes</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Specimen Handling</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Acidosis</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Freezing</term><term>Hydrogen-Ion Concentration</term><term>Reproducibility of Results</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20738495</PMID><DateCreated><Year>2010</Year><Month>08</Month><Day>26</Day></DateCreated><DateCompleted><Year>2011</Year><Month>01</Month><Day>06</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1095-8649</ISSN><JournalIssue CitedMedium="Internet"><Volume>75</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Journal of fish biology</Title><ISOAbbreviation>J. Fish Biol.</ISOAbbreviation></Journal><ArticleTitle>A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method.</ArticleTitle><Pagination><MedlinePgn>268-75</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/j.1095-8649.2009.02261.x</ELocationID><Abstract><AbstractText>This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. 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May</name></noCountry><country name="Canada"><noRegion><name sortKey="Baker, D W" sort="Baker, D W" uniqKey="Baker D" first="D W" last="Baker">D W Baker</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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