A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method
Identifieur interne : 000408 ( Istex/Corpus ); précédent : 000407; suivant : 000409A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method
Auteurs : D. W. Baker ; C. May ; C. J. BraunerSource :
- Journal of Fish Biology [ 0022-1112 ] ; 2009-07.
English descriptors
Abstract
This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).
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DOI: 10.1111/j.1095-8649.2009.02261.x
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BAKER <i>ET AL</i>.</title></titleGroup><creators><creator creatorRole="author" xml:id="cr1" affiliationRef="#a1" corresponding="yes"><personName><givenNames>D.W.</givenNames><familyName>Baker</familyName></personName></creator><creator creatorRole="author" xml:id="cr2" affiliationRef="#a1"><personName><givenNames>C.</givenNames><familyName>May</familyName></personName></creator><creator creatorRole="author" xml:id="cr3" affiliationRef="#a1"><personName><givenNames>C.J.</givenNames><familyName>Brauner</familyName></personName></creator></creators><affiliationGroup><affiliation xml:id="a1" countryCode="CA"><unparsedAffiliation><i>Department of Zoology, 6270 University Blvd, University of British Columbia, Vancouver, B.C., V6T 1Z4 Canada</i></unparsedAffiliation></affiliation></affiliationGroup><keywordGroup xml:lang="en"><keyword xml:id="k1">acid‐base balance</keyword><keyword xml:id="k2"><i>Acipenser transmontanus</i></keyword><keyword xml:id="k3">CO<sub>2</sub></keyword><keyword xml:id="k4">hypercapnia</keyword><keyword xml:id="k5">hypercarbia</keyword><keyword xml:id="k6">tissue pH</keyword></keywordGroup><abstractGroup><abstract type="main" xml:lang="en"><p>This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon <i>Acipenser transmontanus</i> during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO<sub>2</sub> (10000 Pa <i>p</i>CO<sub>2</sub>).</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method</title></titleInfo><name type="personal"><namePart type="given">D.W.</namePart><namePart type="family">Baker</namePart><affiliation>Department of Zoology, 6270 University Blvd, University of British Columbia, Vancouver, B.C., V6T 1Z4 Canada</affiliation><affiliation>E-mail: baker@zoology.ubc.ca</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.</namePart><namePart type="family">May</namePart><affiliation>Department of Zoology, 6270 University Blvd, University of British Columbia, Vancouver, B.C., V6T 1Z4 Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">C.J.</namePart><namePart type="family">Brauner</namePart><affiliation>Department of Zoology, 6270 University Blvd, University of British Columbia, Vancouver, B.C., V6T 1Z4 Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">2009-07</dateIssued><edition>(Received 4 September 2008, Accepted 2 March 2009)</edition><copyrightDate encoding="w3cdtf">2009</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">3</extent><extent unit="references">29</extent></physicalDescription><abstract lang="en">This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).</abstract><subject lang="en"><genre>keywords</genre><topic>acid‐base balance</topic><topic>Acipenser transmontanus</topic><topic>CO2</topic><topic>hypercapnia</topic><topic>hypercarbia</topic><topic>tissue pH</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Fish Biology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0022-1112</identifier><identifier type="eISSN">1095-8649</identifier><identifier type="DOI">10.1111/(ISSN)1095-8649</identifier><identifier type="PublisherID">JFB</identifier><part><date>2009</date><detail type="volume"><caption>vol.</caption><number>75</number></detail><detail type="issue"><caption>no.</caption><number>1</number></detail><extent unit="pages"><start>268</start><end>275</end><total>8</total></extent></part></relatedItem><identifier type="istex">7BA53C4CA565910468834E8B2FC0AB82F5E7690A</identifier><identifier type="DOI">10.1111/j.1095-8649.2009.02261.x</identifier><identifier type="ArticleID">JFB2261</identifier><accessCondition type="use and reproduction" contentType="copyright">© 2009 The Authors. 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