Interactions of the sweet-tasting proteins thaumatin and lysozyme with the human sweet-taste receptor.
Identifieur interne : 000316 ( Main/Exploration ); précédent : 000315; suivant : 000317Interactions of the sweet-tasting proteins thaumatin and lysozyme with the human sweet-taste receptor.
Auteurs : Nobuyuki Ide [Japon] ; Eriko Sato ; Keisuke Ohta ; Tetsuya Masuda ; Naofumi KitabatakeSource :
- Journal of agricultural and food chemistry [ 1520-5118 ] ; 2009.
Descripteurs français
- KwdFr :
- AMP cyclique (analyse), Chlorure de sodium (pharmacologie), Clonage moléculaire (MeSH), Dérivés du benzène (pharmacologie), Expression des gènes (MeSH), Humains (MeSH), Lignée cellulaire (MeSH), Lysozyme (pharmacologie), Protéines végétales (pharmacologie), Rein (MeSH), Récepteurs couplés aux protéines G (effets des médicaments et des substances chimiques), Récepteurs couplés aux protéines G (génétique), Récepteurs couplés aux protéines G (physiologie), Transfection (MeSH), Édulcorants (pharmacologie).
- MESH :
- analyse : AMP cyclique.
- effets des médicaments et des substances chimiques : Récepteurs couplés aux protéines G.
- génétique : Récepteurs couplés aux protéines G.
- pharmacologie : Chlorure de sodium, Dérivés du benzène, Lysozyme, Protéines végétales, Édulcorants.
- physiologie : Récepteurs couplés aux protéines G.
- Clonage moléculaire, Expression des gènes, Humains, Lignée cellulaire, Rein, Transfection.
English descriptors
- KwdEn :
- Benzene Derivatives (pharmacology), Cell Line (MeSH), Cloning, Molecular (MeSH), Cyclic AMP (analysis), Gene Expression (MeSH), Humans (MeSH), Kidney (MeSH), Muramidase (pharmacology), Plant Proteins (pharmacology), Receptors, G-Protein-Coupled (drug effects), Receptors, G-Protein-Coupled (genetics), Receptors, G-Protein-Coupled (physiology), Sodium Chloride (pharmacology), Sweetening Agents (pharmacology), Transfection (MeSH).
- MESH :
- chemical , analysis : Cyclic AMP.
- chemical , drug effects : Receptors, G-Protein-Coupled.
- chemical , genetics : Receptors, G-Protein-Coupled.
- chemical , pharmacology : Benzene Derivatives, Muramidase, Plant Proteins, Sodium Chloride, Sweetening Agents.
- chemical , physiology : Receptors, G-Protein-Coupled.
- Cell Line, Cloning, Molecular, Gene Expression, Humans, Kidney, Transfection.
Abstract
This study investigated the sweetness of the sweet-tasting protein thaumatin and lysozyme by both an in vitro cell-based assay and an in vivo sensory analysis to elucidate the differences between in vitro and in vivo response profiles. Hek293 cells were constructed that stably expressed the human T1R2+T1R3 sweet-taste receptor, and their responses to thaumatin and lysozyme were analyzed by monitoring the levels of intracellular cAMP. The results indicated that thaumatin and lysozyme as well as aspartame induced a decrease in the intracellular cAMP accumulation of the T1R2+T1R3-transfected cells and that EC(50) values of thaumatin and lysozyme determined by cell-based assay are well-consistent with the results of the sweetness threshold value determined by sensory analysis in the presence of 140 mM NaCl. The results of both in vitro and in vivo experiments confirmed that the sweetness inhibitor lactisole significantly suppressed the sweetness of thaumatin and lysozyme.
DOI: 10.1021/jf803956f
PubMed: 19489607
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<author><name sortKey="Sato, Eriko" sort="Sato, Eriko" uniqKey="Sato E" first="Eriko" last="Sato">Eriko Sato</name>
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<author><name sortKey="Ohta, Keisuke" sort="Ohta, Keisuke" uniqKey="Ohta K" first="Keisuke" last="Ohta">Keisuke Ohta</name>
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<author><name sortKey="Masuda, Tetsuya" sort="Masuda, Tetsuya" uniqKey="Masuda T" first="Tetsuya" last="Masuda">Tetsuya Masuda</name>
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<term>Cell Line (MeSH)</term>
<term>Cloning, Molecular (MeSH)</term>
<term>Cyclic AMP (analysis)</term>
<term>Gene Expression (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Kidney (MeSH)</term>
<term>Muramidase (pharmacology)</term>
<term>Plant Proteins (pharmacology)</term>
<term>Receptors, G-Protein-Coupled (drug effects)</term>
<term>Receptors, G-Protein-Coupled (genetics)</term>
<term>Receptors, G-Protein-Coupled (physiology)</term>
<term>Sodium Chloride (pharmacology)</term>
<term>Sweetening Agents (pharmacology)</term>
<term>Transfection (MeSH)</term>
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<term>Chlorure de sodium (pharmacologie)</term>
<term>Clonage moléculaire (MeSH)</term>
<term>Dérivés du benzène (pharmacologie)</term>
<term>Expression des gènes (MeSH)</term>
<term>Humains (MeSH)</term>
<term>Lignée cellulaire (MeSH)</term>
<term>Lysozyme (pharmacologie)</term>
<term>Protéines végétales (pharmacologie)</term>
<term>Rein (MeSH)</term>
<term>Récepteurs couplés aux protéines G (effets des médicaments et des substances chimiques)</term>
<term>Récepteurs couplés aux protéines G (génétique)</term>
<term>Récepteurs couplés aux protéines G (physiologie)</term>
<term>Transfection (MeSH)</term>
<term>Édulcorants (pharmacologie)</term>
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<term>Muramidase</term>
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<term>Sweetening Agents</term>
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<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chlorure de sodium</term>
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<term>Protéines végétales</term>
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<term>Gene Expression</term>
<term>Humans</term>
<term>Kidney</term>
<term>Transfection</term>
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<term>Expression des gènes</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Rein</term>
<term>Transfection</term>
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<front><div type="abstract" xml:lang="en">This study investigated the sweetness of the sweet-tasting protein thaumatin and lysozyme by both an in vitro cell-based assay and an in vivo sensory analysis to elucidate the differences between in vitro and in vivo response profiles. Hek293 cells were constructed that stably expressed the human T1R2+T1R3 sweet-taste receptor, and their responses to thaumatin and lysozyme were analyzed by monitoring the levels of intracellular cAMP. The results indicated that thaumatin and lysozyme as well as aspartame induced a decrease in the intracellular cAMP accumulation of the T1R2+T1R3-transfected cells and that EC(50) values of thaumatin and lysozyme determined by cell-based assay are well-consistent with the results of the sweetness threshold value determined by sensory analysis in the presence of 140 mM NaCl. The results of both in vitro and in vivo experiments confirmed that the sweetness inhibitor lactisole significantly suppressed the sweetness of thaumatin and lysozyme.</div>
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<Abstract><AbstractText>This study investigated the sweetness of the sweet-tasting protein thaumatin and lysozyme by both an in vitro cell-based assay and an in vivo sensory analysis to elucidate the differences between in vitro and in vivo response profiles. Hek293 cells were constructed that stably expressed the human T1R2+T1R3 sweet-taste receptor, and their responses to thaumatin and lysozyme were analyzed by monitoring the levels of intracellular cAMP. The results indicated that thaumatin and lysozyme as well as aspartame induced a decrease in the intracellular cAMP accumulation of the T1R2+T1R3-transfected cells and that EC(50) values of thaumatin and lysozyme determined by cell-based assay are well-consistent with the results of the sweetness threshold value determined by sensory analysis in the presence of 140 mM NaCl. The results of both in vitro and in vivo experiments confirmed that the sweetness inhibitor lactisole significantly suppressed the sweetness of thaumatin and lysozyme.</AbstractText>
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