Micropreparation of single secretory glands from the carnivorous plant Nepenthes.
Identifieur interne : 000315 ( Main/Exploration ); précédent : 000314; suivant : 000316Micropreparation of single secretory glands from the carnivorous plant Nepenthes.
Auteurs : Sandy Rottloff [Allemagne] ; Ute Müller ; Roland Kilper ; Axel MithöferSource :
- Analytical biochemistry [ 1096-0309 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- anatomie et histologie : Magnoliopsida.
- génétique : Magnoliopsida.
- méthodes : Microdissection.
- Facteurs temps, Gènes de plante, Microscopie, Réaction de polymérisation en chaîne, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- MESH :
- anatomy & histology : Magnoliopsida.
- genetics : Magnoliopsida.
- methods : Microdissection.
- Gene Expression Regulation, Plant, Genes, Plant, Microscopy, Polymerase Chain Reaction, Time Factors.
Abstract
A rapid mechanical micropreparation technique has been developed to isolate multicellular glands, here from Nepenthes pitchers, based on a microdissection platform. The method is an alternative to laser capture dissection because fresh plant tissue can be used directly without previous fixation. Subsequent experiments, such as polymerase chain reaction (PCR)-based detection of an individual gene encoding a thaumatin-like protein and RNA extraction for gene expression analysis, have been successfully added to prove the quality of the prepared biological material. The procedure described is adaptable to a broad range of plant species and should find wide application in the preparation of multicellular glands or other tissues.
DOI: 10.1016/j.ab.2009.07.013
PubMed: 19602419
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Roland" uniqKey="Kilper R" first="Roland" last="Kilper">Roland Kilper</name></author><author><name sortKey="Mithofer, Axel" sort="Mithofer, Axel" uniqKey="Mithofer A" first="Axel" last="Mithöfer">Axel Mithöfer</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19602419</idno><idno type="pmid">19602419</idno><idno type="doi">10.1016/j.ab.2009.07.013</idno><idno type="wicri:Area/Main/Corpus">000309</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000309</idno><idno type="wicri:Area/Main/Curation">000309</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000309</idno><idno type="wicri:Area/Main/Exploration">000309</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Micropreparation of single secretory glands from the carnivorous plant Nepenthes.</title><author><name sortKey="Rottloff, Sandy" 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last="Mithöfer">Axel Mithöfer</name></author></analytic><series><title level="j">Analytical biochemistry</title><idno type="eISSN">1096-0309</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Gene Expression Regulation, Plant (MeSH)</term><term>Genes, Plant (MeSH)</term><term>Magnoliopsida (anatomy & histology)</term><term>Magnoliopsida (genetics)</term><term>Microdissection (methods)</term><term>Microscopy (MeSH)</term><term>Polymerase Chain Reaction (MeSH)</term><term>Time Factors (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Facteurs temps (MeSH)</term><term>Gènes de plante (MeSH)</term><term>Magnoliopsida (anatomie et histologie)</term><term>Magnoliopsida (génétique)</term><term>Microdissection (méthodes)</term><term>Microscopie (MeSH)</term><term>Réaction de polymérisation en chaîne (MeSH)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term></keywords><keywords scheme="MESH" qualifier="anatomie et histologie" xml:lang="fr"><term>Magnoliopsida</term></keywords><keywords scheme="MESH" qualifier="anatomy & histology" xml:lang="en"><term>Magnoliopsida</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Magnoliopsida</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Magnoliopsida</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microdissection</term></keywords><keywords scheme="MESH" qualifier="méthodes" xml:lang="fr"><term>Microdissection</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Genes, Plant</term><term>Microscopy</term><term>Polymerase Chain Reaction</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Facteurs temps</term><term>Gènes de plante</term><term>Microscopie</term><term>Réaction de polymérisation en chaîne</term><term>Régulation de l'expression des gènes végétaux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A rapid mechanical micropreparation technique has been developed to isolate multicellular glands, here from Nepenthes pitchers, based on a microdissection platform. The method is an alternative to laser capture dissection because fresh plant tissue can be used directly without previous fixation. Subsequent experiments, such as polymerase chain reaction (PCR)-based detection of an individual gene encoding a thaumatin-like protein and RNA extraction for gene expression analysis, have been successfully added to prove the quality of the prepared biological material. The procedure described is adaptable to a broad range of plant species and should find wide application in the preparation of multicellular glands or other tissues.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19602419</PMID><DateCompleted><Year>2009</Year><Month>11</Month><Day>02</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1096-0309</ISSN><JournalIssue CitedMedium="Internet"><Volume>394</Volume><Issue>1</Issue><PubDate><Year>2009</Year><Month>Nov</Month><Day>01</Day></PubDate></JournalIssue><Title>Analytical biochemistry</Title><ISOAbbreviation>Anal Biochem</ISOAbbreviation></Journal><ArticleTitle>Micropreparation of single secretory glands from the carnivorous plant Nepenthes.</ArticleTitle><Pagination><MedlinePgn>135-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.ab.2009.07.013</ELocationID><Abstract><AbstractText>A rapid mechanical micropreparation technique has been developed to isolate multicellular glands, here from Nepenthes pitchers, based on a microdissection platform. The method is an alternative to laser capture dissection because fresh plant tissue can be used directly without previous fixation. Subsequent experiments, such as polymerase chain reaction (PCR)-based detection of an individual gene encoding a thaumatin-like protein and RNA extraction for gene expression analysis, have been successfully added to prove the quality of the prepared biological material. The procedure described is adaptable to a broad range of plant species and should find wide application in the preparation of multicellular glands or other tissues.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Rottloff</LastName><ForeName>Sandy</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Bioorganic Chemistry, Max Planck Institute for Chemical Ecology, Hans-Knöll-Str. 8, D-07745 Jena, Germany.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Müller</LastName><ForeName>Ute</ForeName><Initials>U</Initials></Author><Author ValidYN="Y"><LastName>Kilper</LastName><ForeName>Roland</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Mithöfer</LastName><ForeName>Axel</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2009</Year><Month>07</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Anal Biochem</MedlineTA><NlmUniqueID>0370535</NlmUniqueID><ISSNLinking>0003-2697</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019684" MajorTopicYN="N">Magnoliopsida</DescriptorName><QualifierName UI="Q000033" MajorTopicYN="Y">anatomy & histology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D042282" MajorTopicYN="N">Microdissection</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008853" MajorTopicYN="N">Microscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2009</Year><Month>04</Month><Day>01</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2009</Year><Month>07</Month><Day>08</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2009</Year><Month>07</Month><Day>09</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>7</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>7</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>11</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19602419</ArticleId><ArticleId IdType="pii">S0003-2697(09)00487-4</ArticleId><ArticleId IdType="doi">10.1016/j.ab.2009.07.013</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Allemagne</li></country></list><tree><noCountry><name sortKey="Kilper, Roland" sort="Kilper, Roland" uniqKey="Kilper R" first="Roland" last="Kilper">Roland Kilper</name><name sortKey="Mithofer, Axel" sort="Mithofer, Axel" uniqKey="Mithofer A" first="Axel" last="Mithöfer">Axel Mithöfer</name><name sortKey="Muller, Ute" sort="Muller, Ute" uniqKey="Muller U" first="Ute" last="Müller">Ute Müller</name></noCountry><country name="Allemagne"><noRegion><name sortKey="Rottloff, Sandy" sort="Rottloff, Sandy" uniqKey="Rottloff S" first="Sandy" last="Rottloff">Sandy Rottloff</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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