Micropreparation of single secretory glands from the carnivorous plant Nepenthes.
Identifieur interne : 000315 ( Main/Exploration ); précédent : 000314; suivant : 000316Micropreparation of single secretory glands from the carnivorous plant Nepenthes.
Auteurs : Sandy Rottloff [Allemagne] ; Ute Müller ; Roland Kilper ; Axel MithöferSource :
- Analytical biochemistry [ 1096-0309 ] ; 2009.
Descripteurs français
- KwdFr :
- MESH :
- anatomie et histologie : Magnoliopsida.
- génétique : Magnoliopsida.
- méthodes : Microdissection.
- Facteurs temps, Gènes de plante, Microscopie, Réaction de polymérisation en chaîne, Régulation de l'expression des gènes végétaux.
English descriptors
- KwdEn :
- MESH :
- anatomy & histology : Magnoliopsida.
- genetics : Magnoliopsida.
- methods : Microdissection.
- Gene Expression Regulation, Plant, Genes, Plant, Microscopy, Polymerase Chain Reaction, Time Factors.
Abstract
A rapid mechanical micropreparation technique has been developed to isolate multicellular glands, here from Nepenthes pitchers, based on a microdissection platform. The method is an alternative to laser capture dissection because fresh plant tissue can be used directly without previous fixation. Subsequent experiments, such as polymerase chain reaction (PCR)-based detection of an individual gene encoding a thaumatin-like protein and RNA extraction for gene expression analysis, have been successfully added to prove the quality of the prepared biological material. The procedure described is adaptable to a broad range of plant species and should find wide application in the preparation of multicellular glands or other tissues.
DOI: 10.1016/j.ab.2009.07.013
PubMed: 19602419
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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<author><name sortKey="Muller, Ute" sort="Muller, Ute" uniqKey="Muller U" first="Ute" last="Müller">Ute Müller</name>
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<term>Magnoliopsida (anatomy & histology)</term>
<term>Magnoliopsida (genetics)</term>
<term>Microdissection (methods)</term>
<term>Microscopy (MeSH)</term>
<term>Polymerase Chain Reaction (MeSH)</term>
<term>Time Factors (MeSH)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>Facteurs temps (MeSH)</term>
<term>Gènes de plante (MeSH)</term>
<term>Magnoliopsida (anatomie et histologie)</term>
<term>Magnoliopsida (génétique)</term>
<term>Microdissection (méthodes)</term>
<term>Microscopie (MeSH)</term>
<term>Réaction de polymérisation en chaîne (MeSH)</term>
<term>Régulation de l'expression des gènes végétaux (MeSH)</term>
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<term>Time Factors</term>
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<term>Gènes de plante</term>
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<front><div type="abstract" xml:lang="en">A rapid mechanical micropreparation technique has been developed to isolate multicellular glands, here from Nepenthes pitchers, based on a microdissection platform. The method is an alternative to laser capture dissection because fresh plant tissue can be used directly without previous fixation. Subsequent experiments, such as polymerase chain reaction (PCR)-based detection of an individual gene encoding a thaumatin-like protein and RNA extraction for gene expression analysis, have been successfully added to prove the quality of the prepared biological material. The procedure described is adaptable to a broad range of plant species and should find wide application in the preparation of multicellular glands or other tissues.</div>
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<Abstract><AbstractText>A rapid mechanical micropreparation technique has been developed to isolate multicellular glands, here from Nepenthes pitchers, based on a microdissection platform. The method is an alternative to laser capture dissection because fresh plant tissue can be used directly without previous fixation. Subsequent experiments, such as polymerase chain reaction (PCR)-based detection of an individual gene encoding a thaumatin-like protein and RNA extraction for gene expression analysis, have been successfully added to prove the quality of the prepared biological material. The procedure described is adaptable to a broad range of plant species and should find wide application in the preparation of multicellular glands or other tissues.</AbstractText>
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