Cloning, mapping and protein expression of wheat thaumatin protein gene (TaTLP1).
Identifieur interne : 000475 ( Main/Exploration ); précédent : 000474; suivant : 000476Cloning, mapping and protein expression of wheat thaumatin protein gene (TaTLP1).
Auteurs : Ling Yu [République populaire de Chine] ; Ji-Shan Niu ; Zheng-Qiang Ma ; Pei-Du Chen ; Da-Jun LiuSource :
- Yi chuan xue bao = Acta genetica Sinica [ 0379-4172 ] ; 2003.
Descripteurs français
- KwdFr :
- ADN complémentaire (composition chimique), ADN complémentaire (génétique), ARN des plantes (génétique), ARN des plantes (métabolisme), Analyse de séquence d'ADN (MeSH), Cartographie chromosomique (MeSH), Chromosomes de plante (génétique), Clonage moléculaire (MeSH), Données de séquences moléculaires (MeSH), Feuilles de plante (génétique), Feuilles de plante (métabolisme), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Technique de Northern (MeSH), Technique de Western (MeSH), Triticum (génétique), Édulcorants (métabolisme).
- MESH :
- composition chimique : ADN complémentaire.
- génétique : ADN complémentaire, ARN des plantes, Chromosomes de plante, Feuilles de plante, Protéines végétales, Triticum.
- métabolisme : ARN des plantes, Feuilles de plante, Protéines végétales, Édulcorants.
- Analyse de séquence d'ADN, Cartographie chromosomique, Clonage moléculaire, Données de séquences moléculaires, Régulation de l'expression des gènes végétaux, Séquence d'acides aminés, Séquence nucléotidique, Technique de Northern, Technique de Western.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Blotting, Northern (MeSH), Blotting, Western (MeSH), Chromosome Mapping (MeSH), Chromosomes, Plant (genetics), Cloning, Molecular (MeSH), DNA, Complementary (chemistry), DNA, Complementary (genetics), Gene Expression Regulation, Plant (MeSH), Molecular Sequence Data (MeSH), Plant Leaves (genetics), Plant Leaves (metabolism), Plant Proteins (genetics), Plant Proteins (metabolism), RNA, Plant (genetics), RNA, Plant (metabolism), Sequence Analysis, DNA (MeSH), Sweetening Agents (metabolism), Triticum (genetics).
- MESH :
- chemical , chemistry : DNA, Complementary.
- genetics : Chromosomes, Plant, DNA, Complementary, Plant Leaves, Plant Proteins, RNA, Plant, Triticum.
- metabolism : Plant Leaves, Plant Proteins, RNA, Plant, Sweetening Agents.
- Amino Acid Sequence, Base Sequence, Blotting, Northern, Blotting, Western, Chromosome Mapping, Cloning, Molecular, Gene Expression Regulation, Plant, Molecular Sequence Data, Sequence Analysis, DNA.
Abstract
To understand wheat powdery mildew resistance mechanism, reverse-transcription polymerase chain reaction (RT-PCR) and cDNA library screening were performed to isolate the full-length cDNA of wheat thaumatin protein gene from wheat-Haynaldia villosa 6VS/6AL translocation line. The putative amino acid sequence of this gene consists of 173 amino acid residues, and is an acid polypeptide. It was highly homologous to thaumatin proteins isolated from other plants, so it is designated as TaTLP1 (GenBank accession number: AF384146). Northern blot analysis of TaTLP1 showed that the transcription difference obviously existed between resistant wheat-Haynaldia villosa 6VS/6AL translocation line and susceptible "Yangmai 5". The result of western blot showed that the protein expression product of TaTLP1 gene in wheat seedling leaf was a soluble cytoplasm protein, whose expression was induced by fungus Erysiph graminis and apparently related to disease resistance of the 6VS/6AL translocation line. Southern blot indicated that the TaTLP1 gene had 1-2 copies in wheat genome, and had been localized on the specific region of 7B and 7D chromosomes in wheat.
PubMed: 12812076
Affiliations:
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sortKey="Chen, Pei Du" sort="Chen, Pei Du" uniqKey="Chen P" first="Pei-Du" last="Chen">Pei-Du Chen</name></author><author><name sortKey="Liu, Da Jun" sort="Liu, Da Jun" uniqKey="Liu D" first="Da-Jun" last="Liu">Da-Jun Liu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:12812076</idno><idno type="pmid">12812076</idno><idno type="wicri:Area/Main/Corpus">000470</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000470</idno><idno type="wicri:Area/Main/Curation">000470</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000470</idno><idno type="wicri:Area/Main/Exploration">000470</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Cloning, mapping and protein expression of wheat thaumatin protein gene (TaTLP1).</title><author><name sortKey="Yu, Ling" sort="Yu, Ling" uniqKey="Yu L" first="Ling" last="Yu">Ling Yu</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Crop Cytogenetics, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Crop Cytogenetics, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095</wicri:regionArea><wicri:noRegion>Nanjing 210095</wicri:noRegion></affiliation></author><author><name sortKey="Niu, Ji Shan" sort="Niu, Ji Shan" uniqKey="Niu J" first="Ji-Shan" last="Niu">Ji-Shan Niu</name></author><author><name sortKey="Ma, Zheng Qiang" sort="Ma, Zheng Qiang" uniqKey="Ma Z" first="Zheng-Qiang" last="Ma">Zheng-Qiang Ma</name></author><author><name sortKey="Chen, Pei Du" sort="Chen, Pei Du" uniqKey="Chen P" first="Pei-Du" last="Chen">Pei-Du Chen</name></author><author><name sortKey="Liu, Da Jun" sort="Liu, Da Jun" uniqKey="Liu D" first="Da-Jun" last="Liu">Da-Jun Liu</name></author></analytic><series><title level="j">Yi chuan xue bao = Acta genetica Sinica</title><idno type="ISSN">0379-4172</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Blotting, Northern (MeSH)</term><term>Blotting, Western (MeSH)</term><term>Chromosome Mapping (MeSH)</term><term>Chromosomes, Plant (genetics)</term><term>Cloning, Molecular (MeSH)</term><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (genetics)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Leaves (genetics)</term><term>Plant Leaves (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>RNA, Plant (genetics)</term><term>RNA, Plant (metabolism)</term><term>Sequence Analysis, DNA (MeSH)</term><term>Sweetening Agents (metabolism)</term><term>Triticum (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (composition chimique)</term><term>ADN complémentaire (génétique)</term><term>ARN des plantes (génétique)</term><term>ARN des plantes (métabolisme)</term><term>Analyse de séquence d'ADN (MeSH)</term><term>Cartographie chromosomique (MeSH)</term><term>Chromosomes de plante (génétique)</term><term>Clonage moléculaire (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Feuilles de plante (génétique)</term><term>Feuilles de plante (métabolisme)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Technique de Northern (MeSH)</term><term>Technique de Western (MeSH)</term><term>Triticum (génétique)</term><term>Édulcorants (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>ADN complémentaire</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Chromosomes, Plant</term><term>DNA, Complementary</term><term>Plant Leaves</term><term>Plant Proteins</term><term>RNA, Plant</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ARN des plantes</term><term>Chromosomes de plante</term><term>Feuilles de plante</term><term>Protéines végétales</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plant Leaves</term><term>Plant Proteins</term><term>RNA, Plant</term><term>Sweetening Agents</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN des plantes</term><term>Feuilles de plante</term><term>Protéines végétales</term><term>Édulcorants</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Blotting, Northern</term><term>Blotting, Western</term><term>Chromosome Mapping</term><term>Cloning, Molecular</term><term>Gene Expression Regulation, Plant</term><term>Molecular Sequence Data</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Cartographie chromosomique</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Régulation de l'expression des gènes végétaux</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Technique de Northern</term><term>Technique de Western</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To understand wheat powdery mildew resistance mechanism, reverse-transcription polymerase chain reaction (RT-PCR) and cDNA library screening were performed to isolate the full-length cDNA of wheat thaumatin protein gene from wheat-Haynaldia villosa 6VS/6AL translocation line. The putative amino acid sequence of this gene consists of 173 amino acid residues, and is an acid polypeptide. It was highly homologous to thaumatin proteins isolated from other plants, so it is designated as TaTLP1 (GenBank accession number: AF384146). Northern blot analysis of TaTLP1 showed that the transcription difference obviously existed between resistant wheat-Haynaldia villosa 6VS/6AL translocation line and susceptible "Yangmai 5". The result of western blot showed that the protein expression product of TaTLP1 gene in wheat seedling leaf was a soluble cytoplasm protein, whose expression was induced by fungus Erysiph graminis and apparently related to disease resistance of the 6VS/6AL translocation line. Southern blot indicated that the TaTLP1 gene had 1-2 copies in wheat genome, and had been localized on the specific region of 7B and 7D chromosomes in wheat.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12812076</PMID><DateCompleted><Year>2003</Year><Month>08</Month><Day>19</Day></DateCompleted><DateRevised><Year>2006</Year><Month>11</Month><Day>15</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0379-4172</ISSN><JournalIssue CitedMedium="Print"><Volume>30</Volume><Issue>1</Issue><PubDate><Year>2003</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Yi chuan xue bao = Acta genetica Sinica</Title><ISOAbbreviation>Yi Chuan Xue Bao</ISOAbbreviation></Journal><ArticleTitle>Cloning, mapping and protein expression of wheat thaumatin protein gene (TaTLP1).</ArticleTitle><Pagination><MedlinePgn>49-55</MedlinePgn></Pagination><Abstract><AbstractText>To understand wheat powdery mildew resistance mechanism, reverse-transcription polymerase chain reaction (RT-PCR) and cDNA library screening were performed to isolate the full-length cDNA of wheat thaumatin protein gene from wheat-Haynaldia villosa 6VS/6AL translocation line. The putative amino acid sequence of this gene consists of 173 amino acid residues, and is an acid polypeptide. It was highly homologous to thaumatin proteins isolated from other plants, so it is designated as TaTLP1 (GenBank accession number: AF384146). Northern blot analysis of TaTLP1 showed that the transcription difference obviously existed between resistant wheat-Haynaldia villosa 6VS/6AL translocation line and susceptible "Yangmai 5". The result of western blot showed that the protein expression product of TaTLP1 gene in wheat seedling leaf was a soluble cytoplasm protein, whose expression was induced by fungus Erysiph graminis and apparently related to disease resistance of the 6VS/6AL translocation line. Southern blot indicated that the TaTLP1 gene had 1-2 copies in wheat genome, and had been localized on the specific region of 7B and 7D chromosomes in wheat.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Ling</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>Key Laboratory of Crop Cytogenetics, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Niu</LastName><ForeName>Ji-Shan</ForeName><Initials>JS</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Zheng-Qiang</ForeName><Initials>ZQ</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Pei-Du</ForeName><Initials>PD</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Da-Jun</ForeName><Initials>DJ</Initials></Author></AuthorList><Language>eng</Language><DataBankList CompleteYN="Y"><DataBank><DataBankName>GENBANK</DataBankName><AccessionNumberList><AccessionNumber>AF384146</AccessionNumber></AccessionNumberList></DataBank></DataBankList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Yi Chuan Xue Bao</MedlineTA><NlmUniqueID>7900784</NlmUniqueID><ISSNLinking>0379-4172</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, Complementary</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018749">RNA, Plant</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013549">Sweetening 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PubStatus="medline"><Year>2003</Year><Month>8</Month><Day>20</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>6</Month><Day>19</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12812076</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Pei Du" sort="Chen, Pei Du" uniqKey="Chen P" first="Pei-Du" last="Chen">Pei-Du Chen</name><name sortKey="Liu, Da Jun" sort="Liu, Da Jun" uniqKey="Liu D" first="Da-Jun" last="Liu">Da-Jun Liu</name><name sortKey="Ma, Zheng Qiang" sort="Ma, Zheng Qiang" uniqKey="Ma Z" first="Zheng-Qiang" last="Ma">Zheng-Qiang Ma</name><name sortKey="Niu, Ji Shan" sort="Niu, Ji Shan" uniqKey="Niu J" first="Ji-Shan" last="Niu">Ji-Shan Niu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yu, Ling" sort="Yu, Ling" uniqKey="Yu L" first="Ling" last="Yu">Ling Yu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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