Constitutive expression of Vitis vinifera thaumatin-like protein after in vitro selection and its role in anthracnose resistance.
Identifieur interne : 000474 ( Main/Exploration ); précédent : 000473; suivant : 000475Constitutive expression of Vitis vinifera thaumatin-like protein after in vitro selection and its role in anthracnose resistance.
Auteurs : Subramanian Jayasankar [Canada] ; Zhijian Li [États-Unis] ; Dennis J. Gray [États-Unis]Source :
- Functional plant biology : FPB [ 1445-4416 ] ; 2003.
Abstract
Anthracnose-resistant grapevine (Vitis vinifera L. cv. Chardonnay) plants were regenerated from embryogenic cultures that had been subjected to in vitro selection with culture filtrate of Elsinoe ampelina (de Bary) Shear. Three secreted proteins differentially expressed by in vitro-selected embryogenic cultures and regenerated plants were identified. An 8-kDa protein was identified as a lipid-transfer protein (LTP) by N-terminal amino acid sequence comparison. Two other differentially expressed proteins, with estimated molecular weights of 21.6 and 22kDa, immuno-reacted with antiserum raised against a thaumatin-like protein (TLP) protein from pinto bean (Phaseolus vulgaris L.). N-terminal amino acid sequencing of the 21.6-kDa protein showed a high degree of homology to V. vinifera thaumatin-like protein 2 (VVTL-2 = grapevine osmotin; Acc no. CAA71883), and that of the 22-kDa protein was homologous to V. vinifera thaumatin-like protein 1 (VVTL-1; AAB61590). Interestingly, both VVTL-1 and VVTL-2 are pathogenesis-related (PR) proteins, belonging to the PR-5 group. Protein produced from the cloned grapevine VVTL-1 gene significantly inhibited E. ampelina spore germination and hyphal growth in vitro. Plants regenerated from in vitro-selected cultures similarly inhibited fungal growth in vivo. Enhanced expression of antifungal VVTL-1 in anthracnose resistant grapevine strongly suggests that it plays an important role, either alone or in conjunction with other PR proteins, by suppressing pathogen growth.
DOI: 10.1071/FP03066
PubMed: 32689093
Affiliations:
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Current address: Department of Plant Agriculture - Vineland Campus, University of Guelph, 4890VictoriaAvenue N, PO Box 7000, Vineland Station, Ontario</wicri:regionArea><wicri:noRegion>Ontario</wicri:noRegion></affiliation></author><author><name sortKey="Li, Zhijian" sort="Li, Zhijian" uniqKey="Li Z" first="Zhijian" last="Li">Zhijian Li</name><affiliation wicri:level="2"><nlm:affiliation>Mid-Florida Research and Education Center, Institute of Food Agricultural Sciences University of Florida, 2725Binion Road, Apopka, FL 32703, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mid-Florida Research and Education Center, Institute of Food Agricultural Sciences University of Florida, 2725Binion Road, Apopka, FL 32703</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author><author><name sortKey="Gray, Dennis J" sort="Gray, Dennis J" uniqKey="Gray D" first="Dennis J" last="Gray">Dennis J. 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Current address: Department of Plant Agriculture - Vineland Campus, University of Guelph, 4890VictoriaAvenue N, PO Box 7000, Vineland Station, Ontario, Canada L0R 2E0.</nlm:affiliation><country>Canada</country><wicri:regionArea>Mid-Florida Research and Education Center, Institute of Food Agricultural Sciences University of Florida, 2725Binion Road, Apopka, FL 32703, USA. 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Gray</name><affiliation wicri:level="2"><nlm:affiliation>Mid-Florida Research and Education Center, Institute of Food Agricultural Sciences University of Florida, 2725Binion Road, Apopka, FL 32703, USA. Corresponding author; email: djg@mail.ifas.ufl.edu.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Mid-Florida Research and Education Center, Institute of Food Agricultural Sciences University of Florida, 2725Binion Road, Apopka, FL 32703</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j">Functional plant biology : FPB</title><idno type="eISSN">1445-4416</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Anthracnose-resistant grapevine (Vitis vinifera L. cv. Chardonnay) plants were regenerated from embryogenic cultures that had been subjected to in vitro selection with culture filtrate of Elsinoe ampelina (de Bary) Shear. Three secreted proteins differentially expressed by in vitro-selected embryogenic cultures and regenerated plants were identified. An 8-kDa protein was identified as a lipid-transfer protein (LTP) by N-terminal amino acid sequence comparison. Two other differentially expressed proteins, with estimated molecular weights of 21.6 and 22kDa, immuno-reacted with antiserum raised against a thaumatin-like protein (TLP) protein from pinto bean (Phaseolus vulgaris L.). N-terminal amino acid sequencing of the 21.6-kDa protein showed a high degree of homology to V. vinifera thaumatin-like protein 2 (VVTL-2 = grapevine osmotin; Acc no. CAA71883), and that of the 22-kDa protein was homologous to V. vinifera thaumatin-like protein 1 (VVTL-1; AAB61590). Interestingly, both VVTL-1 and VVTL-2 are pathogenesis-related (PR) proteins, belonging to the PR-5 group. Protein produced from the cloned grapevine VVTL-1 gene significantly inhibited E. ampelina spore germination and hyphal growth in vitro. Plants regenerated from in vitro-selected cultures similarly inhibited fungal growth in vivo. Enhanced expression of antifungal VVTL-1 in anthracnose resistant grapevine strongly suggests that it plays an important role, either alone or in conjunction with other PR proteins, by suppressing pathogen growth.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32689093</PMID><DateRevised><Year>2020</Year><Month>07</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1445-4416</ISSN><JournalIssue CitedMedium="Internet"><Volume>30</Volume><Issue>11</Issue><PubDate><Year>2003</Year><Month>Jan</Month></PubDate></JournalIssue><Title>Functional plant biology : FPB</Title><ISOAbbreviation>Funct Plant Biol</ISOAbbreviation></Journal><ArticleTitle>Constitutive expression of Vitis vinifera thaumatin-like protein after in vitro selection and its role in anthracnose resistance.</ArticleTitle><Pagination><MedlinePgn>1105-1115</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1071/FP03066</ELocationID><Abstract><AbstractText>Anthracnose-resistant grapevine (Vitis vinifera L. cv. Chardonnay) plants were regenerated from embryogenic cultures that had been subjected to in vitro selection with culture filtrate of Elsinoe ampelina (de Bary) Shear. Three secreted proteins differentially expressed by in vitro-selected embryogenic cultures and regenerated plants were identified. An 8-kDa protein was identified as a lipid-transfer protein (LTP) by N-terminal amino acid sequence comparison. Two other differentially expressed proteins, with estimated molecular weights of 21.6 and 22kDa, immuno-reacted with antiserum raised against a thaumatin-like protein (TLP) protein from pinto bean (Phaseolus vulgaris L.). N-terminal amino acid sequencing of the 21.6-kDa protein showed a high degree of homology to V. vinifera thaumatin-like protein 2 (VVTL-2 = grapevine osmotin; Acc no. CAA71883), and that of the 22-kDa protein was homologous to V. vinifera thaumatin-like protein 1 (VVTL-1; AAB61590). Interestingly, both VVTL-1 and VVTL-2 are pathogenesis-related (PR) proteins, belonging to the PR-5 group. Protein produced from the cloned grapevine VVTL-1 gene significantly inhibited E. ampelina spore germination and hyphal growth in vitro. Plants regenerated from in vitro-selected cultures similarly inhibited fungal growth in vivo. Enhanced expression of antifungal VVTL-1 in anthracnose resistant grapevine strongly suggests that it plays an important role, either alone or in conjunction with other PR proteins, by suppressing pathogen growth.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jayasankar</LastName><ForeName>Subramanian</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Mid-Florida Research and Education Center, Institute of Food Agricultural Sciences University of Florida, 2725Binion Road, Apopka, FL 32703, USA. 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Corresponding author; email: djg@mail.ifas.ufl.edu.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Australia</Country><MedlineTA>Funct Plant Biol</MedlineTA><NlmUniqueID>101154361</NlmUniqueID><ISSNLinking>1445-4416</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2020</Year><Month>7</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>1</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">32689093</ArticleId><ArticleId IdType="pii">FP03066</ArticleId><ArticleId IdType="doi">10.1071/FP03066</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="Canada"><noRegion><name sortKey="Jayasankar, Subramanian" sort="Jayasankar, Subramanian" uniqKey="Jayasankar S" first="Subramanian" last="Jayasankar">Subramanian Jayasankar</name></noRegion></country><country name="États-Unis"><region name="Floride"><name sortKey="Li, Zhijian" sort="Li, Zhijian" uniqKey="Li Z" first="Zhijian" last="Li">Zhijian Li</name></region><name sortKey="Gray, Dennis J" sort="Gray, Dennis J" uniqKey="Gray D" first="Dennis J" last="Gray">Dennis J. 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