Antifungal Activity of an Abundant Thaumatin-Like Protein from Banana against Penicillium expansum, and Its Possible Mechanisms of Action.
Identifieur interne : 000072 ( Main/Exploration ); précédent : 000071; suivant : 000073Antifungal Activity of an Abundant Thaumatin-Like Protein from Banana against Penicillium expansum, and Its Possible Mechanisms of Action.
Auteurs : Wenxiao Jiao [République populaire de Chine] ; Xiangxin Li [République populaire de Chine] ; Handong Zhao [République populaire de Chine] ; Jiankang Cao [République populaire de Chine] ; Weibo Jiang [République populaire de Chine]Source :
- Molecules (Basel, Switzerland) [ 1420-3049 ] ; 2018.
Descripteurs français
- KwdFr :
- Antifongiques (isolement et purification), Antifongiques (pharmacologie), Chromatographie d'échange d'ions (MeSH), Chromatographie en phase inverse (MeSH), Concentration en ions d'hydrogène (MeSH), Masse moléculaire (MeSH), Musa (métabolisme), Paroi cellulaire (effets des médicaments et des substances chimiques), Penicillium (effets des médicaments et des substances chimiques), Penicillium (physiologie), Perméabilité (MeSH), Protéines végétales (isolement et purification), Protéines végétales (pharmacologie), Spectrométrie de masse MALDI (MeSH), Spores fongiques (effets des médicaments et des substances chimiques).
- MESH :
- effets des médicaments et des substances chimiques : Paroi cellulaire, Penicillium, Spores fongiques.
- isolement et purification : Antifongiques, Protéines végétales.
- métabolisme : Musa.
- pharmacologie : Antifongiques, Protéines végétales.
- physiologie : Penicillium.
- Chromatographie d'échange d'ions, Chromatographie en phase inverse, Concentration en ions d'hydrogène, Masse moléculaire, Perméabilité, Spectrométrie de masse MALDI.
English descriptors
- KwdEn :
- Antifungal Agents (isolation & purification), Antifungal Agents (pharmacology), Cell Wall (drug effects), Chromatography, Ion Exchange (MeSH), Chromatography, Reverse-Phase (MeSH), Hydrogen-Ion Concentration (MeSH), Molecular Weight (MeSH), Musa (metabolism), Penicillium (drug effects), Penicillium (physiology), Permeability (MeSH), Plant Proteins (isolation & purification), Plant Proteins (pharmacology), Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH), Spores, Fungal (drug effects).
- MESH :
- chemical , isolation & purification : Antifungal Agents, Plant Proteins.
- chemical , pharmacology : Antifungal Agents, Plant Proteins.
- drug effects : Cell Wall, Penicillium, Spores, Fungal.
- metabolism : Musa.
- physiology : Penicillium.
- Chromatography, Ion Exchange, Chromatography, Reverse-Phase, Hydrogen-Ion Concentration, Molecular Weight, Permeability, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
Abstract
Thaumatin-like protein from banana (designated BanTLP) has been purified by employing a simple protocol consisting of diethylaminoethyl Sephadex (DEAE⁻Sephadex) chromatography, gel filtration on Sephadex G50, and reversed-phase chromatography. The purified protein was identified by MALDI-TOF mass spectrometry, with an estimated molecular weight of 22.1 kDa. BanTLP effectively inhibited in vitro spore germination of Penicillium expansum, one of the main postharvest pathogens in fruits. This study further investigated the antifungal properties and underlying mechanisms of BanTLP against P. expansum. Results demonstrated that BanTLP exhibited antifungal activity in a wide pH range (4.0⁻10.0) at 20⁻50 °C. Propidium iodide (PI) influx and potassium release confirmed that BanTLP induced membrane disruption of the test pathogen, increasing the membrane permeability and disintegration of the cell. This led to cell death, as evidenced by the assays of thiobarbituric acid-reactive species (TBARS) content, the production of reactive oxygen species (ROS), and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence integrity. Ultrastructural alterations in P. expansum conidia after BanTLP treatment revealed severe damage to the cell wall. These results suggest that BanTLP purified from banana exerts antifungal activity against P. expansum by inducing plasma membrane disturbance and cell wall disorganization.
DOI: 10.3390/molecules23061442
PubMed: 29899211
PubMed Central: PMC6099679
Affiliations:
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type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">Molecules (Basel, Switzerland)</title><idno type="eISSN">1420-3049</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antifungal Agents (isolation & purification)</term><term>Antifungal Agents (pharmacology)</term><term>Cell Wall (drug effects)</term><term>Chromatography, Ion Exchange (MeSH)</term><term>Chromatography, Reverse-Phase (MeSH)</term><term>Hydrogen-Ion Concentration (MeSH)</term><term>Molecular Weight (MeSH)</term><term>Musa (metabolism)</term><term>Penicillium (drug effects)</term><term>Penicillium (physiology)</term><term>Permeability (MeSH)</term><term>Plant Proteins (isolation & purification)</term><term>Plant Proteins (pharmacology)</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (MeSH)</term><term>Spores, Fungal (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Antifongiques (isolement et purification)</term><term>Antifongiques (pharmacologie)</term><term>Chromatographie d'échange d'ions (MeSH)</term><term>Chromatographie en phase inverse (MeSH)</term><term>Concentration en ions d'hydrogène (MeSH)</term><term>Masse moléculaire (MeSH)</term><term>Musa (métabolisme)</term><term>Paroi cellulaire (effets des médicaments et des substances chimiques)</term><term>Penicillium (effets des médicaments et des substances chimiques)</term><term>Penicillium (physiologie)</term><term>Perméabilité (MeSH)</term><term>Protéines végétales (isolement et purification)</term><term>Protéines végétales (pharmacologie)</term><term>Spectrométrie de masse MALDI (MeSH)</term><term>Spores fongiques (effets des médicaments et des substances chimiques)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antifungal Agents</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antifungal Agents</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Wall</term><term>Penicillium</term><term>Spores, Fungal</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Paroi cellulaire</term><term>Penicillium</term><term>Spores fongiques</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antifongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Musa</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Musa</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antifongiques</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Penicillium</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Penicillium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, Ion Exchange</term><term>Chromatography, Reverse-Phase</term><term>Hydrogen-Ion Concentration</term><term>Molecular Weight</term><term>Permeability</term><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie d'échange d'ions</term><term>Chromatographie en phase inverse</term><term>Concentration en ions d'hydrogène</term><term>Masse moléculaire</term><term>Perméabilité</term><term>Spectrométrie de masse MALDI</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Thaumatin-like protein from banana (designated BanTLP) has been purified by employing a simple protocol consisting of diethylaminoethyl Sephadex (DEAE⁻Sephadex) chromatography, gel filtration on Sephadex G50, and reversed-phase chromatography. The purified protein was identified by MALDI-TOF mass spectrometry, with an estimated molecular weight of 22.1 kDa. BanTLP effectively inhibited in vitro spore germination of <i>Penicillium expansum</i>, one of the main postharvest pathogens in fruits. This study further investigated the antifungal properties and underlying mechanisms of BanTLP against <i>P. expansum</i>. Results demonstrated that BanTLP exhibited antifungal activity in a wide pH range (4.0⁻10.0) at 20⁻50 °C. Propidium iodide (PI) influx and potassium release confirmed that BanTLP induced membrane disruption of the test pathogen, increasing the membrane permeability and disintegration of the cell. This led to cell death, as evidenced by the assays of thiobarbituric acid-reactive species (TBARS) content, the production of reactive oxygen species (ROS), and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence integrity. Ultrastructural alterations in <i>P. expansum</i> conidia after BanTLP treatment revealed severe damage to the cell wall. These results suggest that BanTLP purified from banana exerts antifungal activity against <i>P. expansum</i> by inducing plasma membrane disturbance and cell wall disorganization.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29899211</PMID><DateCompleted><Year>2018</Year><Month>10</Month><Day>29</Day></DateCompleted><DateRevised><Year>2019</Year><Month>07</Month><Day>25</Day></DateRevised><Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">1420-3049</ISSN><JournalIssue CitedMedium="Internet"><Volume>23</Volume><Issue>6</Issue><PubDate><Year>2018</Year><Month>06</Month><Day>14</Day></PubDate></JournalIssue><Title>Molecules (Basel, Switzerland)</Title><ISOAbbreviation>Molecules</ISOAbbreviation></Journal><ArticleTitle>Antifungal Activity of an Abundant Thaumatin-Like Protein from Banana against <i>Penicillium expansum</i>, and Its Possible Mechanisms of Action.</ArticleTitle><ELocationID EIdType="pii" ValidYN="Y">E1442</ELocationID><ELocationID EIdType="doi" ValidYN="Y">10.3390/molecules23061442</ELocationID><Abstract><AbstractText>Thaumatin-like protein from banana (designated BanTLP) has been purified by employing a simple protocol consisting of diethylaminoethyl Sephadex (DEAE⁻Sephadex) chromatography, gel filtration on Sephadex G50, and reversed-phase chromatography. The purified protein was identified by MALDI-TOF mass spectrometry, with an estimated molecular weight of 22.1 kDa. BanTLP effectively inhibited in vitro spore germination of <i>Penicillium expansum</i>, one of the main postharvest pathogens in fruits. This study further investigated the antifungal properties and underlying mechanisms of BanTLP against <i>P. expansum</i>. Results demonstrated that BanTLP exhibited antifungal activity in a wide pH range (4.0⁻10.0) at 20⁻50 °C. Propidium iodide (PI) influx and potassium release confirmed that BanTLP induced membrane disruption of the test pathogen, increasing the membrane permeability and disintegration of the cell. This led to cell death, as evidenced by the assays of thiobarbituric acid-reactive species (TBARS) content, the production of reactive oxygen species (ROS), and 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence integrity. Ultrastructural alterations in <i>P. expansum</i> conidia after BanTLP treatment revealed severe damage to the cell wall. These results suggest that BanTLP purified from banana exerts antifungal activity against <i>P. expansum</i> by inducing plasma membrane disturbance and cell wall disorganization.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jiao</LastName><ForeName>Wenxiao</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghuadonglu Road, Beijing 100083, China. DC_COCOA@OUTLOOK.COM.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xiangxin</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghuadonglu Road, Beijing 100083, China. lixiangxin2016@163.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhao</LastName><ForeName>Handong</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghuadonglu Road, Beijing 100083, China. h_d_zhao@sina.com.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Jiankang</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghuadonglu Road, Beijing 100083, China. cjk@cau.edu.cn.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Weibo</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>College of Food Science and Nutritional Engineering, China Agricultural University, 17 Qinghuadonglu Road, Beijing 100083, China. jwb@cau.edu.cn.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>06</Month><Day>14</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Molecules</MedlineTA><NlmUniqueID>100964009</NlmUniqueID><ISSNLinking>1420-3049</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000935">Antifungal Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C420118">thaumatin-like protein, Musa acuminata</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000935" MajorTopicYN="N">Antifungal Agents</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002473" MajorTopicYN="N">Cell Wall</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002852" MajorTopicYN="N">Chromatography, Ion Exchange</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D056148" MajorTopicYN="N">Chromatography, Reverse-Phase</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D028521" MajorTopicYN="N">Musa</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010407" MajorTopicYN="N">Penicillium</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010539" MajorTopicYN="N">Permeability</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013172" MajorTopicYN="N">Spores, Fungal</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="Y">Penicillium expansum</Keyword><Keyword MajorTopicYN="Y">antifungal activity</Keyword><Keyword MajorTopicYN="Y">banana</Keyword><Keyword MajorTopicYN="Y">mechanisms of action</Keyword><Keyword MajorTopicYN="Y">thaumatin-like protein</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>05</Month><Day>07</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>05</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>05</Month><Day>29</Day></PubMedPubDate><PubMedPubDate 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Cao</name><name sortKey="Jiang, Weibo" sort="Jiang, Weibo" uniqKey="Jiang W" first="Weibo" last="Jiang">Weibo Jiang</name><name sortKey="Li, Xiangxin" sort="Li, Xiangxin" uniqKey="Li X" first="Xiangxin" last="Li">Xiangxin Li</name><name sortKey="Zhao, Handong" sort="Zhao, Handong" uniqKey="Zhao H" first="Handong" last="Zhao">Handong Zhao</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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