High Incidence of Preharvest Colonization of Huanglongbing-Symptomatic Citrus sinensis Fruit by Lasiodiplodia theobromae (Diplodia natalensis) and Exacerbation of Postharvest Fruit Decay by That Fungus
Identifieur interne : 001880 ( Ncbi/Curation ); précédent : 001879; suivant : 001881High Incidence of Preharvest Colonization of Huanglongbing-Symptomatic Citrus sinensis Fruit by Lasiodiplodia theobromae (Diplodia natalensis) and Exacerbation of Postharvest Fruit Decay by That Fungus
Auteurs : Wei Zhao ; Jinhe Bai ; Greg Mccollum ; Elizabeth BaldwinSource :
- Applied and Environmental Microbiology [ 0099-2240 ] ; 2014.
English descriptors
- KwdEn :
- MESH :
- isolation & purification : Ascomycota.
- microbiology : Citrus sinensis, Fruit, Plant Diseases.
- Microscopy, Electron, Scanning, Real-Time Polymerase Chain Reaction.
Abstract
Huanglongbing (HLB), presumably caused by the bacterium “
Url:
DOI: 10.1128/AEM.02972-14
PubMed: 25344245
PubMed Central: 4272719
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PMC:4272719Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">High Incidence of Preharvest Colonization of Huanglongbing-Symptomatic <named-content content-type="genus-species">Citrus sinensis</named-content>
Fruit by <named-content content-type="genus-species">Lasiodiplodia theobromae</named-content>
(<named-content content-type="genus-species">Diplodia natalensis</named-content>
) and Exacerbation of Postharvest Fruit Decay by That Fungus</title>
<author><name sortKey="Zhao, Wei" sort="Zhao, Wei" uniqKey="Zhao W" first="Wei" last="Zhao">Wei Zhao</name>
</author>
<author><name sortKey="Bai, Jinhe" sort="Bai, Jinhe" uniqKey="Bai J" first="Jinhe" last="Bai">Jinhe Bai</name>
</author>
<author><name sortKey="Mccollum, Greg" sort="Mccollum, Greg" uniqKey="Mccollum G" first="Greg" last="Mccollum">Greg Mccollum</name>
</author>
<author><name sortKey="Baldwin, Elizabeth" sort="Baldwin, Elizabeth" uniqKey="Baldwin E" first="Elizabeth" last="Baldwin">Elizabeth Baldwin</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">25344245</idno>
<idno type="pmc">4272719</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4272719</idno>
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<idno type="doi">10.1128/AEM.02972-14</idno>
<date when="2014">2014</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">High Incidence of Preharvest Colonization of Huanglongbing-Symptomatic <named-content content-type="genus-species">Citrus sinensis</named-content>
Fruit by <named-content content-type="genus-species">Lasiodiplodia theobromae</named-content>
(<named-content content-type="genus-species">Diplodia natalensis</named-content>
) and Exacerbation of Postharvest Fruit Decay by That Fungus</title>
<author><name sortKey="Zhao, Wei" sort="Zhao, Wei" uniqKey="Zhao W" first="Wei" last="Zhao">Wei Zhao</name>
</author>
<author><name sortKey="Bai, Jinhe" sort="Bai, Jinhe" uniqKey="Bai J" first="Jinhe" last="Bai">Jinhe Bai</name>
</author>
<author><name sortKey="Mccollum, Greg" sort="Mccollum, Greg" uniqKey="Mccollum G" first="Greg" last="Mccollum">Greg Mccollum</name>
</author>
<author><name sortKey="Baldwin, Elizabeth" sort="Baldwin, Elizabeth" uniqKey="Baldwin E" first="Elizabeth" last="Baldwin">Elizabeth Baldwin</name>
</author>
</analytic>
<series><title level="j">Applied and Environmental Microbiology</title>
<idno type="ISSN">0099-2240</idno>
<idno type="eISSN">1098-5336</idno>
<imprint><date when="2014">2014</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Ascomycota (isolation & purification)</term>
<term>Citrus sinensis (microbiology)</term>
<term>Fruit (microbiology)</term>
<term>Microscopy, Electron, Scanning</term>
<term>Plant Diseases (microbiology)</term>
<term>Real-Time Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Ascomycota</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term>
<term>Fruit</term>
<term>Plant Diseases</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Microscopy, Electron, Scanning</term>
<term>Real-Time Polymerase Chain Reaction</term>
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<front><div type="abstract" xml:lang="en"><p>Huanglongbing (HLB), presumably caused by the bacterium “<named-content content-type="genus-species">Candidatus</named-content>
Liberibacter asiaticus,” is a devastating citrus disease associated with excessive preharvest fruit drop. <named-content content-type="genus-species">Lasiodiplodia theobromae</named-content>
(diplodia) is the causal organism of citrus stem end rot (SER). The pathogen infects citrus fruit under the calyx abscission zone (AZ-C) and is associated with cell wall hydrolytic enzymes similar to plant enzymes involved in abscission. By means of DNA sequencing, diplodia was found in “<italic>Ca</italic>
. Liberibacter asiaticus”-positive juice from HLB-symptomatic fruit (S) but not in “<italic>Ca</italic>
. Liberibacter asiaticus”-negative juice. Therefore, the incidence of diplodia in fruit tissues, the impact on HLB-related postharvest decay, and the implications for HLB-related preharvest fruit drop were investigated in Hamlin and Valencia oranges. Quantitative PCR results (qPCR) revealed a significantly (<italic>P</italic>
< 0.001) greater incidence of diplodia in the AZ-C of HLB-symptomatic (S; “<italic>Ca</italic>
. Liberibacter asiaticus” threshold cycle [<italic>C<sub>T</sub>
</italic>
] of <30) than in the AZ-C of in asymptomatic (AS; “<italic>Ca</italic>
. Liberibacter asiaticus” <italic>C<sub>T</sub>
</italic>
of ≥30) fruit. In agreement with the qPCR results, 2 weeks after exposure to ethylene, the incidences of SER in S fruit were 66.7% (Hamlin) and 58.7% (Valencia), whereas for AS fruit the decay rates were 6.7% (Hamlin) and 5.3% (Valencia). Diplodia colonization of S fruit AZ-C was observed by scanning electron microscopy and confirmed by PCR test and morphology of conidia in isolates from the AZ-C after surface sterilization. Diplodia <italic>C<sub>T</sub>
</italic>
values were negatively correlated with ethylene production (<italic>R</italic>
= −0.838 for Hamlin; <italic>R</italic>
= −0.858 for Valencia) in S fruit, and positively correlated with fruit detachment force (<italic>R</italic>
= 0.855 for Hamlin; <italic>R</italic>
= 0.850 for Valencia), suggesting that diplodia colonization in AZ-C may exacerbate HLB-associated preharvest fruit drop.</p>
</div>
</front>
</TEI>
</record>
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