Antiinflammatory effects of a red orange extract in human keratinocytes treated with interferon-gamma and histamine.
Identifieur interne : 000A33 ( Ncbi/Curation ); précédent : 000A32; suivant : 000A34Antiinflammatory effects of a red orange extract in human keratinocytes treated with interferon-gamma and histamine.
Auteurs : Venera Cardile [Italie] ; Giuseppina Frasca ; Luisa Rizza ; Paolo Rapisarda ; Francesco BoninaSource :
- Phytotherapy research : PTR [ 1099-1573 ] ; 2010.
English descriptors
- KwdEn :
- Anti-Inflammatory Agents (immunology), Anti-Inflammatory Agents (pharmacology), Cell Line, Chemokine CCL2 (metabolism), Citrus sinensis (chemistry), Citrus sinensis (immunology), Enzyme-Linked Immunosorbent Assay, Histamine (immunology), Histamine (pharmacology), Humans, Intercellular Adhesion Molecule-1 (metabolism), Interferon-gamma (immunology), Interferon-gamma (pharmacology), Interleukin-8 (metabolism), Keratinocytes (drug effects), Keratinocytes (immunology), Plant Extracts (immunology), Plant Extracts (pharmacology).
- MESH :
- chemical , immunology : Anti-Inflammatory Agents, Histamine, Interferon-gamma, Plant Extracts.
- chemical , metabolism : Chemokine CCL2, Intercellular Adhesion Molecule-1, Interleukin-8.
- chemical , pharmacology : Anti-Inflammatory Agents, Histamine, Interferon-gamma, Plant Extracts.
- chemistry : Citrus sinensis.
- drug effects : Keratinocytes.
- immunology : Citrus sinensis, Keratinocytes.
- Cell Line, Enzyme-Linked Immunosorbent Assay, Humans.
Abstract
Red oranges are an important component of the so-called Mediterranean diet and they have been used by traditional medicine for their health protective properties, particularly to heal sore throat and cough, suggesting an interesting antiinflammatory activity. The purpose of this study was to evaluate the antiinflammatory activity of a red orange (Citrus sinensis varieties: Moro, Tarocco, Sanguinello) complex (ROC), characterized by high levels of anthocyanins, flavanones, hydroxycinnamic acids and ascorbic acid, on the human keratinocyte line NCTC 2544 exposed to interferon-gamma (IFN-gamma) and histamine. The expression of immunomodulatory membrane molecules such as inter-cellular adhesion molecule-1 (ICAM-1) by Western blot analysis, and the release of chemokines such as monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) through ELISA kits, were determined. ICAM-1 modulates the permanence and activation of T lymphocytes in the epidermis. MCP-1 is a specific chemoattractant for monocytes and dendritic cells. IL-8 is important for the recruitment of both neutrophils and T lymphocytes. Addition of ROC at different concentrations together with IFN-gamma and histamine induced a dose-dependent inhibition of ICAM-1 expression and MCP-1 and IL-8 release. ROC shows interesting antiinflammatory properties in human keratinocyte cells NCTC 2544. This natural complex could have a topical employment and mitigate the consequences of some skin pathologies.
DOI: 10.1002/ptr.2973
PubMed: 19655299
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pubmed:19655299Le document en format XML
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<author><name sortKey="Cardile, Venera" sort="Cardile, Venera" uniqKey="Cardile V" first="Venera" last="Cardile">Venera Cardile</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Physiological Sciences, University of Catania, V. le A. Doria 6, 95125 Catania, Italy. cardile@unict.it</nlm:affiliation>
<country xml:lang="fr">Italie</country>
<wicri:regionArea>Department of Physiological Sciences, University of Catania, V. le A. Doria 6, 95125 Catania</wicri:regionArea>
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<author><name sortKey="Frasca, Giuseppina" sort="Frasca, Giuseppina" uniqKey="Frasca G" first="Giuseppina" last="Frasca">Giuseppina Frasca</name>
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<author><name sortKey="Rizza, Luisa" sort="Rizza, Luisa" uniqKey="Rizza L" first="Luisa" last="Rizza">Luisa Rizza</name>
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<author><name sortKey="Rapisarda, Paolo" sort="Rapisarda, Paolo" uniqKey="Rapisarda P" first="Paolo" last="Rapisarda">Paolo Rapisarda</name>
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<author><name sortKey="Bonina, Francesco" sort="Bonina, Francesco" uniqKey="Bonina F" first="Francesco" last="Bonina">Francesco Bonina</name>
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<author><name sortKey="Rizza, Luisa" sort="Rizza, Luisa" uniqKey="Rizza L" first="Luisa" last="Rizza">Luisa Rizza</name>
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<author><name sortKey="Rapisarda, Paolo" sort="Rapisarda, Paolo" uniqKey="Rapisarda P" first="Paolo" last="Rapisarda">Paolo Rapisarda</name>
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<series><title level="j">Phytotherapy research : PTR</title>
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<term>Chemokine CCL2 (metabolism)</term>
<term>Citrus sinensis (chemistry)</term>
<term>Citrus sinensis (immunology)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Histamine (immunology)</term>
<term>Histamine (pharmacology)</term>
<term>Humans</term>
<term>Intercellular Adhesion Molecule-1 (metabolism)</term>
<term>Interferon-gamma (immunology)</term>
<term>Interferon-gamma (pharmacology)</term>
<term>Interleukin-8 (metabolism)</term>
<term>Keratinocytes (drug effects)</term>
<term>Keratinocytes (immunology)</term>
<term>Plant Extracts (immunology)</term>
<term>Plant Extracts (pharmacology)</term>
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<term>Histamine</term>
<term>Interferon-gamma</term>
<term>Plant Extracts</term>
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<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Chemokine CCL2</term>
<term>Intercellular Adhesion Molecule-1</term>
<term>Interleukin-8</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Anti-Inflammatory Agents</term>
<term>Histamine</term>
<term>Interferon-gamma</term>
<term>Plant Extracts</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term>
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<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Keratinocytes</term>
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<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Citrus sinensis</term>
<term>Keratinocytes</term>
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<term>Enzyme-Linked Immunosorbent Assay</term>
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<front><div type="abstract" xml:lang="en">Red oranges are an important component of the so-called Mediterranean diet and they have been used by traditional medicine for their health protective properties, particularly to heal sore throat and cough, suggesting an interesting antiinflammatory activity. The purpose of this study was to evaluate the antiinflammatory activity of a red orange (Citrus sinensis varieties: Moro, Tarocco, Sanguinello) complex (ROC), characterized by high levels of anthocyanins, flavanones, hydroxycinnamic acids and ascorbic acid, on the human keratinocyte line NCTC 2544 exposed to interferon-gamma (IFN-gamma) and histamine. The expression of immunomodulatory membrane molecules such as inter-cellular adhesion molecule-1 (ICAM-1) by Western blot analysis, and the release of chemokines such as monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) through ELISA kits, were determined. ICAM-1 modulates the permanence and activation of T lymphocytes in the epidermis. MCP-1 is a specific chemoattractant for monocytes and dendritic cells. IL-8 is important for the recruitment of both neutrophils and T lymphocytes. Addition of ROC at different concentrations together with IFN-gamma and histamine induced a dose-dependent inhibition of ICAM-1 expression and MCP-1 and IL-8 release. ROC shows interesting antiinflammatory properties in human keratinocyte cells NCTC 2544. This natural complex could have a topical employment and mitigate the consequences of some skin pathologies.</div>
</front>
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