Antiinflammatory effects of a red orange extract in human keratinocytes treated with interferon-gamma and histamine.
Identifieur interne : 000A33 ( Ncbi/Merge ); précédent : 000A32; suivant : 000A34Antiinflammatory effects of a red orange extract in human keratinocytes treated with interferon-gamma and histamine.
Auteurs : Venera Cardile [Italie] ; Giuseppina Frasca ; Luisa Rizza ; Paolo Rapisarda ; Francesco BoninaSource :
- Phytotherapy research : PTR [ 1099-1573 ] ; 2010.
English descriptors
- KwdEn :
- Anti-Inflammatory Agents (immunology), Anti-Inflammatory Agents (pharmacology), Cell Line, Chemokine CCL2 (metabolism), Citrus sinensis (chemistry), Citrus sinensis (immunology), Enzyme-Linked Immunosorbent Assay, Histamine (immunology), Histamine (pharmacology), Humans, Intercellular Adhesion Molecule-1 (metabolism), Interferon-gamma (immunology), Interferon-gamma (pharmacology), Interleukin-8 (metabolism), Keratinocytes (drug effects), Keratinocytes (immunology), Plant Extracts (immunology), Plant Extracts (pharmacology).
- MESH :
- chemical , immunology : Anti-Inflammatory Agents, Histamine, Interferon-gamma, Plant Extracts.
- chemical , metabolism : Chemokine CCL2, Intercellular Adhesion Molecule-1, Interleukin-8.
- chemical , pharmacology : Anti-Inflammatory Agents, Histamine, Interferon-gamma, Plant Extracts.
- chemistry : Citrus sinensis.
- drug effects : Keratinocytes.
- immunology : Citrus sinensis, Keratinocytes.
- Cell Line, Enzyme-Linked Immunosorbent Assay, Humans.
Abstract
Red oranges are an important component of the so-called Mediterranean diet and they have been used by traditional medicine for their health protective properties, particularly to heal sore throat and cough, suggesting an interesting antiinflammatory activity. The purpose of this study was to evaluate the antiinflammatory activity of a red orange (Citrus sinensis varieties: Moro, Tarocco, Sanguinello) complex (ROC), characterized by high levels of anthocyanins, flavanones, hydroxycinnamic acids and ascorbic acid, on the human keratinocyte line NCTC 2544 exposed to interferon-gamma (IFN-gamma) and histamine. The expression of immunomodulatory membrane molecules such as inter-cellular adhesion molecule-1 (ICAM-1) by Western blot analysis, and the release of chemokines such as monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) through ELISA kits, were determined. ICAM-1 modulates the permanence and activation of T lymphocytes in the epidermis. MCP-1 is a specific chemoattractant for monocytes and dendritic cells. IL-8 is important for the recruitment of both neutrophils and T lymphocytes. Addition of ROC at different concentrations together with IFN-gamma and histamine induced a dose-dependent inhibition of ICAM-1 expression and MCP-1 and IL-8 release. ROC shows interesting antiinflammatory properties in human keratinocyte cells NCTC 2544. This natural complex could have a topical employment and mitigate the consequences of some skin pathologies.
DOI: 10.1002/ptr.2973
PubMed: 19655299
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Doria 6, 95125 Catania</wicri:regionArea><wicri:noRegion>95125 Catania</wicri:noRegion></affiliation></author><author><name sortKey="Frasca, Giuseppina" sort="Frasca, Giuseppina" uniqKey="Frasca G" first="Giuseppina" last="Frasca">Giuseppina Frasca</name></author><author><name sortKey="Rizza, Luisa" sort="Rizza, Luisa" uniqKey="Rizza L" first="Luisa" last="Rizza">Luisa Rizza</name></author><author><name sortKey="Rapisarda, Paolo" sort="Rapisarda, Paolo" uniqKey="Rapisarda P" first="Paolo" last="Rapisarda">Paolo Rapisarda</name></author><author><name sortKey="Bonina, Francesco" sort="Bonina, Francesco" uniqKey="Bonina F" first="Francesco" last="Bonina">Francesco Bonina</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:19655299</idno><idno type="pmid">19655299</idno><idno type="doi">10.1002/ptr.2973</idno><idno type="wicri:Area/PubMed/Corpus">000877</idno><idno type="wicri:Area/PubMed/Curation">000877</idno><idno type="wicri:Area/PubMed/Checkpoint">000877</idno><idno type="wicri:Area/Ncbi/Merge">000A33</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Antiinflammatory effects of a red orange extract in human keratinocytes treated with interferon-gamma and histamine.</title><author><name sortKey="Cardile, Venera" sort="Cardile, Venera" uniqKey="Cardile V" first="Venera" last="Cardile">Venera Cardile</name><affiliation wicri:level="1"><nlm:affiliation>Department of Physiological Sciences, University of Catania, V. le A. Doria 6, 95125 Catania, Italy. cardile@unict.it</nlm:affiliation><country xml:lang="fr">Italie</country><wicri:regionArea>Department of Physiological Sciences, University of Catania, V. le A. 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The purpose of this study was to evaluate the antiinflammatory activity of a red orange (Citrus sinensis varieties: Moro, Tarocco, Sanguinello) complex (ROC), characterized by high levels of anthocyanins, flavanones, hydroxycinnamic acids and ascorbic acid, on the human keratinocyte line NCTC 2544 exposed to interferon-gamma (IFN-gamma) and histamine. The expression of immunomodulatory membrane molecules such as inter-cellular adhesion molecule-1 (ICAM-1) by Western blot analysis, and the release of chemokines such as monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) through ELISA kits, were determined. ICAM-1 modulates the permanence and activation of T lymphocytes in the epidermis. MCP-1 is a specific chemoattractant for monocytes and dendritic cells. IL-8 is important for the recruitment of both neutrophils and T lymphocytes. Addition of ROC at different concentrations together with IFN-gamma and histamine induced a dose-dependent inhibition of ICAM-1 expression and MCP-1 and IL-8 release. ROC shows interesting antiinflammatory properties in human keratinocyte cells NCTC 2544. This natural complex could have a topical employment and mitigate the consequences of some skin pathologies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19655299</PMID><DateCreated><Year>2010</Year><Month>2</Month><Day>26</Day></DateCreated><DateCompleted><Year>2010</Year><Month>04</Month><Day>13</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1099-1573</ISSN><JournalIssue CitedMedium="Internet"><Volume>24</Volume><Issue>3</Issue><PubDate><Year>2010</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Phytotherapy research : PTR</Title><ISOAbbreviation>Phytother Res</ISOAbbreviation></Journal><ArticleTitle>Antiinflammatory effects of a red orange extract in human keratinocytes treated with interferon-gamma and histamine.</ArticleTitle><Pagination><MedlinePgn>414-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/ptr.2973</ELocationID><Abstract><AbstractText>Red oranges are an important component of the so-called Mediterranean diet and they have been used by traditional medicine for their health protective properties, particularly to heal sore throat and cough, suggesting an interesting antiinflammatory activity. The purpose of this study was to evaluate the antiinflammatory activity of a red orange (Citrus sinensis varieties: Moro, Tarocco, Sanguinello) complex (ROC), characterized by high levels of anthocyanins, flavanones, hydroxycinnamic acids and ascorbic acid, on the human keratinocyte line NCTC 2544 exposed to interferon-gamma (IFN-gamma) and histamine. The expression of immunomodulatory membrane molecules such as inter-cellular adhesion molecule-1 (ICAM-1) by Western blot analysis, and the release of chemokines such as monocyte chemoattractant protein-1 (MCP-1) and interleukin-8 (IL-8) through ELISA kits, were determined. ICAM-1 modulates the permanence and activation of T lymphocytes in the epidermis. MCP-1 is a specific chemoattractant for monocytes and dendritic cells. IL-8 is important for the recruitment of both neutrophils and T lymphocytes. Addition of ROC at different concentrations together with IFN-gamma and histamine induced a dose-dependent inhibition of ICAM-1 expression and MCP-1 and IL-8 release. ROC shows interesting antiinflammatory properties in human keratinocyte cells NCTC 2544. This natural complex could have a topical employment and mitigate the consequences of some skin pathologies.</AbstractText><CopyrightInformation>(c) 2009 John Wiley & Sons, Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Cardile</LastName><ForeName>Venera</ForeName><Initials>V</Initials><AffiliationInfo><Affiliation>Department of Physiological Sciences, University of Catania, V. le A. 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MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018799" MajorTopicYN="N">Intercellular Adhesion Molecule-1</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D007371" MajorTopicYN="N">Interferon-gamma</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016209" MajorTopicYN="N">Interleukin-8</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015603" MajorTopicYN="N">Keratinocytes</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>8</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>8</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>4</Month><Day>14</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19655299</ArticleId><ArticleId IdType="doi">10.1002/ptr.2973</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Italie</li></country></list><tree><noCountry><name sortKey="Bonina, Francesco" sort="Bonina, Francesco" uniqKey="Bonina F" first="Francesco" last="Bonina">Francesco Bonina</name><name sortKey="Frasca, Giuseppina" sort="Frasca, Giuseppina" uniqKey="Frasca G" first="Giuseppina" last="Frasca">Giuseppina Frasca</name><name sortKey="Rapisarda, Paolo" sort="Rapisarda, Paolo" uniqKey="Rapisarda P" first="Paolo" last="Rapisarda">Paolo Rapisarda</name><name sortKey="Rizza, Luisa" sort="Rizza, Luisa" uniqKey="Rizza L" first="Luisa" last="Rizza">Luisa Rizza</name></noCountry><country name="Italie"><noRegion><name sortKey="Cardile, Venera" sort="Cardile, Venera" uniqKey="Cardile V" first="Venera" last="Cardile">Venera Cardile</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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