Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine☆
Identifieur interne : 000837 ( Ncbi/Curation ); précédent : 000836; suivant : 000838Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine☆
Auteurs : Karina R. Vega-Villa ; Connie M. Remsberg ; Kristy L. Podelnyk ; Neal M. DaviesSource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1570-0232 ] ; 2008.
Abstract
A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4′-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak® AD™-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 µg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV) and was within 12% at the limit of quantitation (0.5 µg/ml). Bias of the assay was <15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine.
Url:
DOI: 10.1016/j.jchromb.2008.05.018
PubMed: 18514595
PubMed Central: 2917051
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000A63
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000A62
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001057
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000837
Links to Exploration step
PMC:2917051Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine<xref ref-type="fn" rid="FN1">☆</xref></title><author><name sortKey="Vega Villa, Karina R" sort="Vega Villa, Karina R" uniqKey="Vega Villa K" first="Karina R." last="Vega-Villa">Karina R. Vega-Villa</name></author><author><name sortKey="Remsberg, Connie M" sort="Remsberg, Connie M" uniqKey="Remsberg C" first="Connie M." last="Remsberg">Connie M. Remsberg</name></author><author><name sortKey="Podelnyk, Kristy L" sort="Podelnyk, Kristy L" uniqKey="Podelnyk K" first="Kristy L." last="Podelnyk">Kristy L. Podelnyk</name></author><author><name sortKey="Davies, Neal M" sort="Davies, Neal M" uniqKey="Davies N" first="Neal M." last="Davies">Neal M. Davies</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18514595</idno><idno type="pmc">2917051</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2917051</idno><idno type="RBID">PMC:2917051</idno><idno type="doi">10.1016/j.jchromb.2008.05.018</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000A63</idno><idno type="wicri:Area/Pmc/Curation">000A62</idno><idno type="wicri:Area/Pmc/Checkpoint">001057</idno><idno type="wicri:Area/Ncbi/Merge">000837</idno><idno type="wicri:Area/Ncbi/Curation">000837</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine<xref ref-type="fn" rid="FN1">☆</xref></title><author><name sortKey="Vega Villa, Karina R" sort="Vega Villa, Karina R" uniqKey="Vega Villa K" first="Karina R." last="Vega-Villa">Karina R. Vega-Villa</name></author><author><name sortKey="Remsberg, Connie M" sort="Remsberg, Connie M" uniqKey="Remsberg C" first="Connie M." last="Remsberg">Connie M. Remsberg</name></author><author><name sortKey="Podelnyk, Kristy L" sort="Podelnyk, Kristy L" uniqKey="Podelnyk K" first="Kristy L." last="Podelnyk">Kristy L. Podelnyk</name></author><author><name sortKey="Davies, Neal M" sort="Davies, Neal M" uniqKey="Davies N" first="Neal M." last="Davies">Neal M. Davies</name></author></analytic><series><title level="j">Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><idno type="ISSN">1570-0232</idno><idno type="eISSN">1873-376X</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4′-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak<sup>®</sup> AD™-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 µg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV) and was within 12% at the limit of quantitation (0.5 µg/ml). Bias of the assay was <15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine.</p></div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000837 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000837 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= PMC:2917051
|texte= Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine☆
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:18514595" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |