Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine☆
Identifieur interne : 000A63 ( Pmc/Corpus ); précédent : 000A62; suivant : 000A64Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine☆
Auteurs : Karina R. Vega-Villa ; Connie M. Remsberg ; Kristy L. Podelnyk ; Neal M. DaviesSource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1570-0232 ] ; 2008.
Abstract
A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4′-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak® AD™-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 µg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV) and was within 12% at the limit of quantitation (0.5 µg/ml). Bias of the assay was <15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine.
Url:
DOI: 10.1016/j.jchromb.2008.05.018
PubMed: 18514595
PubMed Central: 2917051
Links to Exploration step
PMC:2917051Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine<xref ref-type="fn" rid="FN1">☆</xref></title><author><name sortKey="Vega Villa, Karina R" sort="Vega Villa, Karina R" uniqKey="Vega Villa K" first="Karina R." last="Vega-Villa">Karina R. Vega-Villa</name></author><author><name sortKey="Remsberg, Connie M" sort="Remsberg, Connie M" uniqKey="Remsberg C" first="Connie M." last="Remsberg">Connie M. Remsberg</name></author><author><name sortKey="Podelnyk, Kristy L" sort="Podelnyk, Kristy L" uniqKey="Podelnyk K" first="Kristy L." last="Podelnyk">Kristy L. Podelnyk</name></author><author><name sortKey="Davies, Neal M" sort="Davies, Neal M" uniqKey="Davies N" first="Neal M." last="Davies">Neal M. Davies</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">18514595</idno><idno type="pmc">2917051</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2917051</idno><idno type="RBID">PMC:2917051</idno><idno type="doi">10.1016/j.jchromb.2008.05.018</idno><date when="2008">2008</date><idno type="wicri:Area/Pmc/Corpus">000A63</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine<xref ref-type="fn" rid="FN1">☆</xref></title><author><name sortKey="Vega Villa, Karina R" sort="Vega Villa, Karina R" uniqKey="Vega Villa K" first="Karina R." last="Vega-Villa">Karina R. Vega-Villa</name></author><author><name sortKey="Remsberg, Connie M" sort="Remsberg, Connie M" uniqKey="Remsberg C" first="Connie M." last="Remsberg">Connie M. Remsberg</name></author><author><name sortKey="Podelnyk, Kristy L" sort="Podelnyk, Kristy L" uniqKey="Podelnyk K" first="Kristy L." last="Podelnyk">Kristy L. Podelnyk</name></author><author><name sortKey="Davies, Neal M" sort="Davies, Neal M" uniqKey="Davies N" first="Neal M." last="Davies">Neal M. Davies</name></author></analytic><series><title level="j">Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><idno type="ISSN">1570-0232</idno><idno type="eISSN">1873-376X</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4′-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak<sup>®</sup> AD™-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 µg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV) and was within 12% at the limit of quantitation (0.5 µg/ml). Bias of the assay was <15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine.</p></div></front></TEI><pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101139554</journal-id><journal-id journal-id-type="pubmed-jr-id">27040</journal-id><journal-id journal-id-type="nlm-ta">J Chromatogr B Analyt Technol Biomed Life Sci</journal-id><journal-title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</journal-title><issn pub-type="ppub">1570-0232</issn><issn pub-type="epub">1873-376X</issn></journal-meta><article-meta><article-id pub-id-type="pmid">18514595</article-id><article-id pub-id-type="pmc">2917051</article-id><article-id pub-id-type="doi">10.1016/j.jchromb.2008.05.018</article-id><article-id pub-id-type="manuscript">NIHMS217983</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine<xref ref-type="fn" rid="FN1">☆</xref></article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Vega-Villa</surname><given-names>Karina R.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Remsberg</surname><given-names>Connie M.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Podelnyk</surname><given-names>Kristy L.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Davies</surname><given-names>Neal M.</given-names></name><xref ref-type="corresp" rid="cor1">*</xref></contrib><aff id="A1">College of Pharmacy, Department of Pharmaceutical Sciences and Pharmacology Toxicology Graduate Program, Washington State University, Pullman, Washington 99164-6534, USA</aff></contrib-group><author-notes><corresp id="cor1"><label>*</label>Corresponding author. Tel.: +1 509 335 4754; fax: +1 509 335 5902. <email>ndavies@wsu.edu</email> (N.M. Davies)</corresp></author-notes><pub-date pub-type="nihms-submitted"><day>27</day><month>7</month><year>2010</year></pub-date><pub-date pub-type="ppub"><day>1</day><month>11</month><year>2008</year></pub-date><pub-date pub-type="pmc-release"><day>6</day><month>8</month><year>2010</year></pub-date><volume>875</volume><issue>1</issue><fpage>142</fpage><lpage>147</lpage><permissions><copyright-statement>© 2008 Elsevier B.V. All rights reserved.</copyright-statement><copyright-year>2008</copyright-year></permissions><abstract><p id="P1">A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4′-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak<sup>®</sup> AD™-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 µg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV) and was within 12% at the limit of quantitation (0.5 µg/ml). Bias of the assay was <15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine.</p></abstract><kwd-group><kwd>HPLC</kwd><kwd>UV-detection</kwd><kwd>Stereospecific</kwd><kwd>Enantiomer</kwd><kwd>Flavonoid</kwd></kwd-group><contract-num rid="GM1">T32 GM008336-18
||GM</contract-num><contract-num rid="GM1">T32 GM008336-17
||GM</contract-num><contract-num rid="GM1">T32 GM008336-16
||GM</contract-num><contract-sponsor id="GM1">National Institute of General Medical Sciences : NIGMS</contract-sponsor></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000A63 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000A63 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé= PMC:2917051
|texte= Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine☆
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:18514595" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |