Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine☆
Identifieur interne : 000837 ( Ncbi/Merge ); précédent : 000836; suivant : 000838Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine☆
Auteurs : Karina R. Vega-Villa ; Connie M. Remsberg ; Kristy L. Podelnyk ; Neal M. DaviesSource :
- Journal of chromatography. B, Analytical technologies in the biomedical and life sciences [ 1570-0232 ] ; 2008.
Abstract
A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4′-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak® AD™-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 µg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV) and was within 12% at the limit of quantitation (0.5 µg/ml). Bias of the assay was <15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine.
Url:
DOI: 10.1016/j.jchromb.2008.05.018
PubMed: 18514595
PubMed Central: 2917051
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PMC:2917051Le document en format XML
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<author><name sortKey="Vega Villa, Karina R" sort="Vega Villa, Karina R" uniqKey="Vega Villa K" first="Karina R." last="Vega-Villa">Karina R. Vega-Villa</name>
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<author><name sortKey="Remsberg, Connie M" sort="Remsberg, Connie M" uniqKey="Remsberg C" first="Connie M." last="Remsberg">Connie M. Remsberg</name>
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<author><name sortKey="Podelnyk, Kristy L" sort="Podelnyk, Kristy L" uniqKey="Podelnyk K" first="Kristy L." last="Podelnyk">Kristy L. Podelnyk</name>
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<author><name sortKey="Davies, Neal M" sort="Davies, Neal M" uniqKey="Davies N" first="Neal M." last="Davies">Neal M. Davies</name>
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<author><name sortKey="Vega Villa, Karina R" sort="Vega Villa, Karina R" uniqKey="Vega Villa K" first="Karina R." last="Vega-Villa">Karina R. Vega-Villa</name>
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<series><title level="j">Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title>
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<front><div type="abstract" xml:lang="en"><p id="P1">A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4′-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak<sup>®</sup>
AD™-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 µg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV) and was within 12% at the limit of quantitation (0.5 µg/ml). Bias of the assay was <15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine.</p>
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<pmc article-type="research-article" xml:lang="EN"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<pmc-dir>properties manuscript</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-journal-id">101139554</journal-id>
<journal-id journal-id-type="pubmed-jr-id">27040</journal-id>
<journal-id journal-id-type="nlm-ta">J Chromatogr B Analyt Technol Biomed Life Sci</journal-id>
<journal-title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</journal-title>
<issn pub-type="ppub">1570-0232</issn>
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<article-id pub-id-type="manuscript">NIHMS217983</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
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<title-group><article-title>Stereospecific high-performance liquid chromatographic assay of isosakuranetin in rat urine<xref ref-type="fn" rid="FN1">☆</xref>
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<contrib-group><contrib contrib-type="author"><name><surname>Vega-Villa</surname>
<given-names>Karina R.</given-names>
</name>
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<contrib contrib-type="author"><name><surname>Remsberg</surname>
<given-names>Connie M.</given-names>
</name>
</contrib>
<contrib contrib-type="author"><name><surname>Podelnyk</surname>
<given-names>Kristy L.</given-names>
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<contrib contrib-type="author"><name><surname>Davies</surname>
<given-names>Neal M.</given-names>
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<xref ref-type="corresp" rid="cor1">*</xref>
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<aff id="A1">College of Pharmacy, Department of Pharmaceutical Sciences and Pharmacology Toxicology Graduate Program, Washington State University, Pullman, Washington 99164-6534, USA</aff>
</contrib-group>
<author-notes><corresp id="cor1"><label>*</label>
Corresponding author. Tel.: +1 509 335 4754; fax: +1 509 335 5902. <email>ndavies@wsu.edu</email>
(N.M. Davies)</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>27</day>
<month>7</month>
<year>2010</year>
</pub-date>
<pub-date pub-type="ppub"><day>1</day>
<month>11</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>6</day>
<month>8</month>
<year>2010</year>
</pub-date>
<volume>875</volume>
<issue>1</issue>
<fpage>142</fpage>
<lpage>147</lpage>
<permissions><copyright-statement>© 2008 Elsevier B.V. All rights reserved.</copyright-statement>
<copyright-year>2008</copyright-year>
</permissions>
<abstract><p id="P1">A stereospecific method of analysis of racemic isosakuranetin (5,7-dihydroxy-4′-methoxyflavanone) in biological fluids is necessary to study pharmacokinetics. A simple high-performance liquid chromatographic method was developed for the determination of isosakuranetin enantiomers. Separation was achieved on a Chiralpak<sup>®</sup>
AD™-RH column with ultraviolet (UV)-detection at 286 nm. The standard curves in urine were linear ranging from 0.5 to 100.0 µg/ml for each enantiomer. The mean extraction efficiency was >88.0%. Precision of the assay was <15% (CV) and was within 12% at the limit of quantitation (0.5 µg/ml). Bias of the assay was <15% and was within 6% at the limit of quantitation. The assay was applied successfully to stereospecific disposition of isosakuranetin enantiomers in rat urine.</p>
</abstract>
<kwd-group><kwd>HPLC</kwd>
<kwd>UV-detection</kwd>
<kwd>Stereospecific</kwd>
<kwd>Enantiomer</kwd>
<kwd>Flavonoid</kwd>
</kwd-group>
<contract-num rid="GM1">T32 GM008336-18
||GM</contract-num>
<contract-num rid="GM1">T32 GM008336-17
||GM</contract-num>
<contract-num rid="GM1">T32 GM008336-16
||GM</contract-num>
<contract-sponsor id="GM1">National Institute of General Medical Sciences : NIGMS</contract-sponsor>
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<affiliations><list></list>
<tree><noCountry><name sortKey="Davies, Neal M" sort="Davies, Neal M" uniqKey="Davies N" first="Neal M." last="Davies">Neal M. Davies</name>
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<name sortKey="Remsberg, Connie M" sort="Remsberg, Connie M" uniqKey="Remsberg C" first="Connie M." last="Remsberg">Connie M. Remsberg</name>
<name sortKey="Vega Villa, Karina R" sort="Vega Villa, Karina R" uniqKey="Vega Villa K" first="Karina R." last="Vega-Villa">Karina R. Vega-Villa</name>
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