Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.
Identifieur interne : 001657 ( Main/Exploration ); précédent : 001656; suivant : 001658Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.
Auteurs : Tiago R. Jacob [Brésil] ; Marcelo L. Laia ; Jesus A. Ferro ; Maria I T. FerroSource :
- Biotechnology letters [ 1573-6776 ] ; 2011.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Bacterial Proton-Translocating ATPases (genetics), Base Sequence, Biotechnology, Citrus sinensis (microbiology), DNA Gyrase (genetics), DNA Primers (genetics), DNA-Directed RNA Polymerases (genetics), Gene Expression Profiling (methods), Genes, Bacterial, Plant Diseases (genetics), Plant Diseases (microbiology), RNA, Bacterial (genetics), Reverse Transcriptase Polymerase Chain Reaction, Virulence (genetics), Xanthomonas (genetics), Xanthomonas (pathogenicity).
- MESH :
- chemical , genetics : Bacterial Proteins, Bacterial Proton-Translocating ATPases, DNA Gyrase, DNA Primers, DNA-Directed RNA Polymerases, RNA, Bacterial.
- genetics : Plant Diseases, Virulence, Xanthomonas.
- methods : Gene Expression Profiling.
- microbiology : Citrus sinensis, Plant Diseases.
- pathogenicity : Xanthomonas.
- Base Sequence, Biotechnology, Genes, Bacterial, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.
DOI: 10.1007/s10529-011-0552-5
PubMed: 21318633
Affiliations:
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Jacob</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, 14884-900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, 14884-900</wicri:regionArea><wicri:noRegion>14884-900</wicri:noRegion></affiliation></author><author><name sortKey="Laia, Marcelo L" sort="Laia, Marcelo L" uniqKey="Laia M" first="Marcelo L" last="Laia">Marcelo L. Laia</name></author><author><name sortKey="Ferro, Jesus A" sort="Ferro, Jesus A" uniqKey="Ferro J" first="Jesus A" last="Ferro">Jesus A. Ferro</name></author><author><name sortKey="Ferro, Maria I T" sort="Ferro, Maria I T" uniqKey="Ferro M" first="Maria I T" last="Ferro">Maria I T. 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Jacob</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, 14884-900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, 14884-900</wicri:regionArea><wicri:noRegion>14884-900</wicri:noRegion></affiliation></author><author><name sortKey="Laia, Marcelo L" sort="Laia, Marcelo L" uniqKey="Laia M" first="Marcelo L" last="Laia">Marcelo L. Laia</name></author><author><name sortKey="Ferro, Jesus A" sort="Ferro, Jesus A" uniqKey="Ferro J" first="Jesus A" last="Ferro">Jesus A. Ferro</name></author><author><name sortKey="Ferro, Maria I T" sort="Ferro, Maria I T" uniqKey="Ferro M" first="Maria I T" last="Ferro">Maria I T. Ferro</name></author></analytic><series><title level="j">Biotechnology letters</title><idno type="eISSN">1573-6776</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term><term>Bacterial Proton-Translocating ATPases (genetics)</term><term>Base Sequence</term><term>Biotechnology</term><term>Citrus sinensis (microbiology)</term><term>DNA Gyrase (genetics)</term><term>DNA Primers (genetics)</term><term>DNA-Directed RNA Polymerases (genetics)</term><term>Gene Expression Profiling (methods)</term><term>Genes, Bacterial</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>RNA, Bacterial (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Virulence (genetics)</term><term>Xanthomonas (genetics)</term><term>Xanthomonas (pathogenicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Bacterial Proton-Translocating ATPases</term><term>DNA Gyrase</term><term>DNA Primers</term><term>DNA-Directed RNA Polymerases</term><term>RNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Diseases</term><term>Virulence</term><term>Xanthomonas</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gene Expression Profiling</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Xanthomonas</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Biotechnology</term><term>Genes, Bacterial</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.</div></front></TEI><affiliations><list><country><li>Brésil</li></country></list><tree><noCountry><name sortKey="Ferro, Jesus A" sort="Ferro, Jesus A" uniqKey="Ferro J" first="Jesus A" last="Ferro">Jesus A. Ferro</name><name sortKey="Ferro, Maria I T" sort="Ferro, Maria I T" uniqKey="Ferro M" first="Maria I T" last="Ferro">Maria I T. Ferro</name><name sortKey="Laia, Marcelo L" sort="Laia, Marcelo L" uniqKey="Laia M" first="Marcelo L" last="Laia">Marcelo L. Laia</name></noCountry><country name="Brésil"><noRegion><name sortKey="Jacob, Tiago R" sort="Jacob, Tiago R" uniqKey="Jacob T" first="Tiago R" last="Jacob">Tiago R. Jacob</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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