Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.
Identifieur interne : 001657 ( Main/Curation ); précédent : 001656; suivant : 001658Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.
Auteurs : Tiago R. Jacob [Brésil] ; Marcelo L. Laia ; Jesus A. Ferro ; Maria I T. FerroSource :
- Biotechnology letters [ 1573-6776 ] ; 2011.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Bacterial Proton-Translocating ATPases (genetics), Base Sequence, Biotechnology, Citrus sinensis (microbiology), DNA Gyrase (genetics), DNA Primers (genetics), DNA-Directed RNA Polymerases (genetics), Gene Expression Profiling (methods), Genes, Bacterial, Plant Diseases (genetics), Plant Diseases (microbiology), RNA, Bacterial (genetics), Reverse Transcriptase Polymerase Chain Reaction, Virulence (genetics), Xanthomonas (genetics), Xanthomonas (pathogenicity).
- MESH :
- chemical , genetics : Bacterial Proteins, Bacterial Proton-Translocating ATPases, DNA Gyrase, DNA Primers, DNA-Directed RNA Polymerases, RNA, Bacterial.
- genetics : Plant Diseases, Virulence, Xanthomonas.
- methods : Gene Expression Profiling.
- microbiology : Citrus sinensis, Plant Diseases.
- pathogenicity : Xanthomonas.
- Base Sequence, Biotechnology, Genes, Bacterial, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.
DOI: 10.1007/s10529-011-0552-5
PubMed: 21318633
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pubmed:21318633Le document en format XML
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<series><title level="j">Biotechnology letters</title>
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<term>Citrus sinensis (microbiology)</term>
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<term>DNA Primers (genetics)</term>
<term>DNA-Directed RNA Polymerases (genetics)</term>
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<term>DNA-Directed RNA Polymerases</term>
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<front><div type="abstract" xml:lang="en">Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.</div>
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