Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.
Identifieur interne : 000C97 ( Ncbi/Merge ); précédent : 000C96; suivant : 000C98Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.
Auteurs : Tiago R. Jacob [Brésil] ; Marcelo L. Laia ; Jesus A. Ferro ; Maria I T. FerroSource :
- Biotechnology letters [ 1573-6776 ] ; 2011.
English descriptors
- KwdEn :
- Bacterial Proteins (genetics), Bacterial Proton-Translocating ATPases (genetics), Base Sequence, Biotechnology, Citrus sinensis (microbiology), DNA Gyrase (genetics), DNA Primers (genetics), DNA-Directed RNA Polymerases (genetics), Gene Expression Profiling (methods), Genes, Bacterial, Plant Diseases (genetics), Plant Diseases (microbiology), RNA, Bacterial (genetics), Reverse Transcriptase Polymerase Chain Reaction, Virulence (genetics), Xanthomonas (genetics), Xanthomonas (pathogenicity).
- MESH :
- chemical , genetics : Bacterial Proteins, Bacterial Proton-Translocating ATPases, DNA Gyrase, DNA Primers, DNA-Directed RNA Polymerases, RNA, Bacterial.
- genetics : Plant Diseases, Virulence, Xanthomonas.
- methods : Gene Expression Profiling.
- microbiology : Citrus sinensis, Plant Diseases.
- pathogenicity : Xanthomonas.
- Base Sequence, Biotechnology, Genes, Bacterial, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.
DOI: 10.1007/s10529-011-0552-5
PubMed: 21318633
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 000734
- to stream PubMed, to step Curation: 000734
- to stream PubMed, to step Checkpoint: 000734
Links to Exploration step
pubmed:21318633Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.</title><author><name sortKey="Jacob, Tiago R" sort="Jacob, Tiago R" uniqKey="Jacob T" first="Tiago R" last="Jacob">Tiago R. Jacob</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, 14884-900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, 14884-900</wicri:regionArea><wicri:noRegion>14884-900</wicri:noRegion></affiliation></author><author><name sortKey="Laia, Marcelo L" sort="Laia, Marcelo L" uniqKey="Laia M" first="Marcelo L" last="Laia">Marcelo L. Laia</name></author><author><name sortKey="Ferro, Jesus A" sort="Ferro, Jesus A" uniqKey="Ferro J" first="Jesus A" last="Ferro">Jesus A. Ferro</name></author><author><name sortKey="Ferro, Maria I T" sort="Ferro, Maria I T" uniqKey="Ferro M" first="Maria I T" last="Ferro">Maria I T. Ferro</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21318633</idno><idno type="pmid">21318633</idno><idno type="doi">10.1007/s10529-011-0552-5</idno><idno type="wicri:Area/PubMed/Corpus">000734</idno><idno type="wicri:Area/PubMed/Curation">000734</idno><idno type="wicri:Area/PubMed/Checkpoint">000734</idno><idno type="wicri:Area/Ncbi/Merge">000C97</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.</title><author><name sortKey="Jacob, Tiago R" sort="Jacob, Tiago R" uniqKey="Jacob T" first="Tiago R" last="Jacob">Tiago R. Jacob</name><affiliation wicri:level="1"><nlm:affiliation>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, 14884-900, Brazil.</nlm:affiliation><country xml:lang="fr">Brésil</country><wicri:regionArea>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, 14884-900</wicri:regionArea><wicri:noRegion>14884-900</wicri:noRegion></affiliation></author><author><name sortKey="Laia, Marcelo L" sort="Laia, Marcelo L" uniqKey="Laia M" first="Marcelo L" last="Laia">Marcelo L. Laia</name></author><author><name sortKey="Ferro, Jesus A" sort="Ferro, Jesus A" uniqKey="Ferro J" first="Jesus A" last="Ferro">Jesus A. Ferro</name></author><author><name sortKey="Ferro, Maria I T" sort="Ferro, Maria I T" uniqKey="Ferro M" first="Maria I T" last="Ferro">Maria I T. Ferro</name></author></analytic><series><title level="j">Biotechnology letters</title><idno type="eISSN">1573-6776</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Bacterial Proteins (genetics)</term><term>Bacterial Proton-Translocating ATPases (genetics)</term><term>Base Sequence</term><term>Biotechnology</term><term>Citrus sinensis (microbiology)</term><term>DNA Gyrase (genetics)</term><term>DNA Primers (genetics)</term><term>DNA-Directed RNA Polymerases (genetics)</term><term>Gene Expression Profiling (methods)</term><term>Genes, Bacterial</term><term>Plant Diseases (genetics)</term><term>Plant Diseases (microbiology)</term><term>RNA, Bacterial (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Virulence (genetics)</term><term>Xanthomonas (genetics)</term><term>Xanthomonas (pathogenicity)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Bacterial Proton-Translocating ATPases</term><term>DNA Gyrase</term><term>DNA Primers</term><term>DNA-Directed RNA Polymerases</term><term>RNA, Bacterial</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Plant Diseases</term><term>Virulence</term><term>Xanthomonas</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Gene Expression Profiling</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Citrus sinensis</term><term>Plant Diseases</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Xanthomonas</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Biotechnology</term><term>Genes, Bacterial</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">21318633</PMID><DateCreated><Year>2011</Year><Month>5</Month><Day>16</Day></DateCreated><DateCompleted><Year>2011</Year><Month>08</Month><Day>24</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1573-6776</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>6</Issue><PubDate><Year>2011</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Biotechnology letters</Title><ISOAbbreviation>Biotechnol. Lett.</ISOAbbreviation></Journal><ArticleTitle>Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.</ArticleTitle><Pagination><MedlinePgn>1177-84</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s10529-011-0552-5</ELocationID><Abstract><AbstractText>Xanthomonas citri subsp. citri (Xcc) causes citrus canker, a worldwide disease found mainly in sweet oranges (Citrus sinensis (L.) Osbeck). The expression of nine candidate internal reference genes was analyzed in Xcc grown alone and during C. sinensis infection to identify genes most suitable for comparative expression studies in Xcc using reverse transcription quantitative PCR (qRT-PCR). The stability of these genes was determined using the programs geNorm, NormFinder and BestKeeper. The genes most suitable for data normalization during C. sinensis infection were atpD, rpoB, gyrA and gyrB. The use of at least three reference genes is essential for accurate data normalization in Xcc.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Jacob</LastName><ForeName>Tiago R</ForeName><Initials>TR</Initials><AffiliationInfo><Affiliation>Departamento de Tecnologia, Faculdade de Ciências Agrárias e Veterinárias, UNESP-Univ Estadual Paulista, Campus de Jaboticabal, Jaboticabal, SP, 14884-900, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Laia</LastName><ForeName>Marcelo L</ForeName><Initials>ML</Initials></Author><Author ValidYN="Y"><LastName>Ferro</LastName><ForeName>Jesus A</ForeName><Initials>JA</Initials></Author><Author ValidYN="Y"><LastName>Ferro</LastName><ForeName>Maria I T</ForeName><Initials>MI</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D023361">Validation Studies</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2011</Year><Month>Feb</Month><Day>12</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Biotechnol Lett</MedlineTA><NlmUniqueID>8008051</NlmUniqueID><ISSNLinking>0141-5492</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D001426">Bacterial Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012329">RNA, Bacterial</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.6</RegistryNumber><NameOfSubstance UI="D012321">DNA-Directed RNA Polymerases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.7.7.6</RegistryNumber><NameOfSubstance UI="C105078">RNA polymerase beta subunit</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.6.1.-</RegistryNumber><NameOfSubstance UI="D025243">Bacterial Proton-Translocating ATPases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 5.99.1.3</RegistryNumber><NameOfSubstance UI="D027081">DNA Gyrase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001426" MajorTopicYN="N">Bacterial Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D025243" MajorTopicYN="N">Bacterial Proton-Translocating ATPases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001709" MajorTopicYN="N">Biotechnology</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D027081" MajorTopicYN="N">DNA Gyrase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012321" MajorTopicYN="N">DNA-Directed RNA Polymerases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005798" MajorTopicYN="Y">Genes, Bacterial</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012329" MajorTopicYN="N">RNA, Bacterial</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014974" MajorTopicYN="N">Xanthomonas</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>10</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2011</Year><Month>1</Month><Day>25</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2011</Year><Month>2</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2011</Year><Month>2</Month><Day>15</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>8</Month><Day>25</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">21318633</ArticleId><ArticleId IdType="doi">10.1007/s10529-011-0552-5</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country></list><tree><noCountry><name sortKey="Ferro, Jesus A" sort="Ferro, Jesus A" uniqKey="Ferro J" first="Jesus A" last="Ferro">Jesus A. Ferro</name><name sortKey="Ferro, Maria I T" sort="Ferro, Maria I T" uniqKey="Ferro M" first="Maria I T" last="Ferro">Maria I T. Ferro</name><name sortKey="Laia, Marcelo L" sort="Laia, Marcelo L" uniqKey="Laia M" first="Marcelo L" last="Laia">Marcelo L. Laia</name></noCountry><country name="Brésil"><noRegion><name sortKey="Jacob, Tiago R" sort="Jacob, Tiago R" uniqKey="Jacob T" first="Tiago R" last="Jacob">Tiago R. Jacob</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C97 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000C97 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:21318633
|texte= Selection and validation of reference genes for gene expression studies by reverse transcription quantitative PCR in Xanthomonas citri subsp. citri during infection of Citrus sinensis.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:21318633" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a OrangerV1
| This area was generated with Dilib version V0.6.25. |  |