Analysis of orange juice for total cysteine and glutathione content by CZE with UV-absorption detection.
Identifieur interne : 001E12 ( Main/Exploration ); précédent : 001E11; suivant : 001E13Analysis of orange juice for total cysteine and glutathione content by CZE with UV-absorption detection.
Auteurs : Paweł Kubalczyk [Pologne] ; Edward BaldSource :
- Electrophoresis [ 1522-2683 ] ; 2009.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Cysteine, Glutathione.
- chemical , chemistry : Acetonitriles.
- analysis : Beverages.
- chemistry : Citrus sinensis, Fruit.
- methods : Electrophoresis, Capillary.
- Chromatography, High Pressure Liquid, Reproducibility of Results, Sensitivity and Specificity, Ultraviolet Rays.
Abstract
For total content of cysteine (Cys) and glutathione (GSH) a sample (500 microL) of neat orange juice or commercially available soft drinks is treated according to the procedure consisted of four essential steps: reduction of disulfide forms of the analytes to their thiol counterparts with Tris 2-carboxyethylphosphine, derivatization of thiols to their S-quinolinium derivatives with 2-chloro-1-methylquinolinium tetrafluroborate, separation of so-formed derivatives by capillary zone electrophoresis with ACN stacking, and detection and quantitation with the use of ultraviolet detector at 355 nm. Commercially available bare fused-silica capillary served as the CE column. The method is linear in a wide range of concentrations covering practical application. The coefficient of correlation between analyte concentrations within the range from 2.5 to 30.0 micromol/L and the detector response was for Cys and GSH 0.998 and 0.997, respectively. The imprecision for above-mentioned range was between 6.7 and 3.8, and 3.6 and 1.1% RSD for Cys and GSH, respectively. The method was applied to several samples of fresh juices and commercial orange beverages.
DOI: 10.1002/elps.200800741
PubMed: 19569125
Affiliations:
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Le document en format XML
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<country xml:lang="fr">Pologne</country>
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<series><title level="j">Electrophoresis</title>
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<term>Cysteine (analysis)</term>
<term>Electrophoresis, Capillary (methods)</term>
<term>Fruit (chemistry)</term>
<term>Glutathione (analysis)</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
<term>Ultraviolet Rays</term>
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<front><div type="abstract" xml:lang="en">For total content of cysteine (Cys) and glutathione (GSH) a sample (500 microL) of neat orange juice or commercially available soft drinks is treated according to the procedure consisted of four essential steps: reduction of disulfide forms of the analytes to their thiol counterparts with Tris 2-carboxyethylphosphine, derivatization of thiols to their S-quinolinium derivatives with 2-chloro-1-methylquinolinium tetrafluroborate, separation of so-formed derivatives by capillary zone electrophoresis with ACN stacking, and detection and quantitation with the use of ultraviolet detector at 355 nm. Commercially available bare fused-silica capillary served as the CE column. The method is linear in a wide range of concentrations covering practical application. The coefficient of correlation between analyte concentrations within the range from 2.5 to 30.0 micromol/L and the detector response was for Cys and GSH 0.998 and 0.997, respectively. The imprecision for above-mentioned range was between 6.7 and 3.8, and 3.6 and 1.1% RSD for Cys and GSH, respectively. The method was applied to several samples of fresh juices and commercial orange beverages.</div>
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