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Analysis of orange juice for total cysteine and glutathione content by CZE with UV-absorption detection.

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Analysis of orange juice for total cysteine and glutathione content by CZE with UV-absorption detection.

Auteurs : Paweł Kubalczyk [Pologne] ; Edward Bald

Source :

RBID : pubmed:19569125

English descriptors

Abstract

For total content of cysteine (Cys) and glutathione (GSH) a sample (500 microL) of neat orange juice or commercially available soft drinks is treated according to the procedure consisted of four essential steps: reduction of disulfide forms of the analytes to their thiol counterparts with Tris 2-carboxyethylphosphine, derivatization of thiols to their S-quinolinium derivatives with 2-chloro-1-methylquinolinium tetrafluroborate, separation of so-formed derivatives by capillary zone electrophoresis with ACN stacking, and detection and quantitation with the use of ultraviolet detector at 355 nm. Commercially available bare fused-silica capillary served as the CE column. The method is linear in a wide range of concentrations covering practical application. The coefficient of correlation between analyte concentrations within the range from 2.5 to 30.0 micromol/L and the detector response was for Cys and GSH 0.998 and 0.997, respectively. The imprecision for above-mentioned range was between 6.7 and 3.8, and 3.6 and 1.1% RSD for Cys and GSH, respectively. The method was applied to several samples of fresh juices and commercial orange beverages.

DOI: 10.1002/elps.200800741
PubMed: 19569125

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pubmed:19569125

Le document en format XML

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<nlm:affiliation>Department of Environmental Chemistry, University of Łódź, Łódź, Poland.</nlm:affiliation>
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<name sortKey="Bald, Edward" sort="Bald, Edward" uniqKey="Bald E" first="Edward" last="Bald">Edward Bald</name>
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<term>Beverages (analysis)</term>
<term>Chromatography, High Pressure Liquid</term>
<term>Citrus sinensis (chemistry)</term>
<term>Cysteine (analysis)</term>
<term>Electrophoresis, Capillary (methods)</term>
<term>Fruit (chemistry)</term>
<term>Glutathione (analysis)</term>
<term>Reproducibility of Results</term>
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<div type="abstract" xml:lang="en">For total content of cysteine (Cys) and glutathione (GSH) a sample (500 microL) of neat orange juice or commercially available soft drinks is treated according to the procedure consisted of four essential steps: reduction of disulfide forms of the analytes to their thiol counterparts with Tris 2-carboxyethylphosphine, derivatization of thiols to their S-quinolinium derivatives with 2-chloro-1-methylquinolinium tetrafluroborate, separation of so-formed derivatives by capillary zone electrophoresis with ACN stacking, and detection and quantitation with the use of ultraviolet detector at 355 nm. Commercially available bare fused-silica capillary served as the CE column. The method is linear in a wide range of concentrations covering practical application. The coefficient of correlation between analyte concentrations within the range from 2.5 to 30.0 micromol/L and the detector response was for Cys and GSH 0.998 and 0.997, respectively. The imprecision for above-mentioned range was between 6.7 and 3.8, and 3.6 and 1.1% RSD for Cys and GSH, respectively. The method was applied to several samples of fresh juices and commercial orange beverages.</div>
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