Diversity of endophytic yeasts from sweet orange and their localization by scanning electron microscopy.
Identifieur interne : 000940 ( PubMed/Checkpoint ); précédent : 000939; suivant : 000941Diversity of endophytic yeasts from sweet orange and their localization by scanning electron microscopy.
Auteurs : Cláudia Santos Gai [Brésil] ; Paulo Teixeira Lacava ; Walter Maccheroni ; Chirlei Glienke ; Welington Luiz Araújo ; Thomas Albert Miller ; João Lúcio AzevedoSource :
- Journal of basic microbiology [ 1521-4028 ] ; 2009.
Descripteurs français
- Wicri :
- geographic : Brésil.
English descriptors
- KwdEn :
- Brazil, Citrus sinensis (microbiology), Cryptococcus (genetics), Cryptococcus (isolation & purification), Cryptococcus (metabolism), Cryptococcus (ultrastructure), Culture Media, DNA, Ribosomal Spacer (genetics), Data Interpretation, Statistical, Genes, Plant (genetics), Host-Pathogen Interactions, Microscopy, Electron, Scanning (methods), Phylogeny, Pichia (genetics), Pichia (isolation & purification), Pichia (metabolism), Pichia (ultrastructure), Plant Diseases (microbiology), Plant Leaves (microbiology), Plant Roots (microbiology), Plant Stems (microbiology), Rhodotorula (genetics), Rhodotorula (isolation & purification), Rhodotorula (metabolism), Rhodotorula (ultrastructure), Xylella (growth & development), Xylella (metabolism), Yeasts (genetics), Yeasts (isolation & purification), Yeasts (metabolism), Yeasts (ultrastructure).
- MESH :
- chemical , genetics : DNA, Ribosomal Spacer.
- chemical : Culture Media.
- geographic : Brazil.
- genetics : Cryptococcus, Genes, Plant, Pichia, Rhodotorula, Yeasts.
- growth & development : Xylella.
- isolation & purification : Cryptococcus, Pichia, Rhodotorula, Yeasts.
- metabolism : Cryptococcus, Pichia, Rhodotorula, Xylella, Yeasts.
- methods : Microscopy, Electron, Scanning.
- microbiology : Citrus sinensis, Plant Diseases, Plant Leaves, Plant Roots, Plant Stems.
- ultrastructure : Cryptococcus, Pichia, Rhodotorula, Yeasts.
- Data Interpretation, Statistical, Host-Pathogen Interactions, Phylogeny.
Abstract
Endophytes are microorganisms that colonize plant tissues internally without causing harm to the host. Despite the increasing number of studies on sweet orange pathogens and endophytes, yeast has not been described as a sweet orange endophyte. In the present study, endophytic yeasts were isolated from sweet orange plants and identified by sequencing of internal transcribed spacer (ITS) rRNA. Plants sampled from four different sites in the state of São Paulo, Brazil exhibited different levels of CVC (citrus variegated chlorosis) development. Three citrus endophytic yeasts (CEYs), chosen as representative examples of the isolates observed, were identified as Rhodotorula mucilaginosa, Pichia guilliermondii and Cryptococcus flavescens. These strains were inoculated into axenic Citrus sinensis seedlings. After 45 days, endophytes were re-isolated in populations ranging from 10(6) to 10(9) CFU/g of plant tissue, but, in spite of the high concentrations of yeast cells, no disease symptoms were observed. Colonized plant material was examined by scanning electron microscopy (SEM), and yeast cells were found mainly in the stomata and xylem of plants, reinforcing their endophytic nature. P. guilliermondii was isolated primarily from plants colonized by the causal agent of CVC, Xylella fastidiosa. The supernatant from a culture of P. guilliermondii increased the in vitro growth of X. fastidiosa, suggesting that the yeast could assist in the establishment of this pathogen in its host plant and, therefore, contribute to the development of disease symptoms.
DOI: 10.1002/jobm.200800328
PubMed: 19798655
Affiliations:
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Miller</name></author><author><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name></author></analytic><series><title level="j">Journal of basic microbiology</title><idno type="eISSN">1521-4028</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Brazil</term><term>Citrus sinensis (microbiology)</term><term>Cryptococcus (genetics)</term><term>Cryptococcus (isolation & purification)</term><term>Cryptococcus (metabolism)</term><term>Cryptococcus (ultrastructure)</term><term>Culture Media</term><term>DNA, Ribosomal Spacer (genetics)</term><term>Data Interpretation, Statistical</term><term>Genes, Plant (genetics)</term><term>Host-Pathogen Interactions</term><term>Microscopy, Electron, Scanning (methods)</term><term>Phylogeny</term><term>Pichia (genetics)</term><term>Pichia (isolation & purification)</term><term>Pichia (metabolism)</term><term>Pichia (ultrastructure)</term><term>Plant Diseases (microbiology)</term><term>Plant Leaves (microbiology)</term><term>Plant Roots (microbiology)</term><term>Plant Stems (microbiology)</term><term>Rhodotorula (genetics)</term><term>Rhodotorula (isolation & purification)</term><term>Rhodotorula (metabolism)</term><term>Rhodotorula (ultrastructure)</term><term>Xylella (growth & development)</term><term>Xylella (metabolism)</term><term>Yeasts (genetics)</term><term>Yeasts (isolation & purification)</term><term>Yeasts (metabolism)</term><term>Yeasts (ultrastructure)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Ribosomal Spacer</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Brazil</term></keywords><keywords 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xml:lang="en"><term>Cryptococcus</term><term>Pichia</term><term>Rhodotorula</term><term>Yeasts</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Data Interpretation, Statistical</term><term>Host-Pathogen Interactions</term><term>Phylogeny</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Brésil</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Endophytes are microorganisms that colonize plant tissues internally without causing harm to the host. Despite the increasing number of studies on sweet orange pathogens and endophytes, yeast has not been described as a sweet orange endophyte. In the present study, endophytic yeasts were isolated from sweet orange plants and identified by sequencing of internal transcribed spacer (ITS) rRNA. Plants sampled from four different sites in the state of São Paulo, Brazil exhibited different levels of CVC (citrus variegated chlorosis) development. Three citrus endophytic yeasts (CEYs), chosen as representative examples of the isolates observed, were identified as Rhodotorula mucilaginosa, Pichia guilliermondii and Cryptococcus flavescens. These strains were inoculated into axenic Citrus sinensis seedlings. After 45 days, endophytes were re-isolated in populations ranging from 10(6) to 10(9) CFU/g of plant tissue, but, in spite of the high concentrations of yeast cells, no disease symptoms were observed. Colonized plant material was examined by scanning electron microscopy (SEM), and yeast cells were found mainly in the stomata and xylem of plants, reinforcing their endophytic nature. P. guilliermondii was isolated primarily from plants colonized by the causal agent of CVC, Xylella fastidiosa. The supernatant from a culture of P. guilliermondii increased the in vitro growth of X. fastidiosa, suggesting that the yeast could assist in the establishment of this pathogen in its host plant and, therefore, contribute to the development of disease symptoms.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19798655</PMID><DateCreated><Year>2009</Year><Month>10</Month><Day>6</Day></DateCreated><DateCompleted><Year>2010</Year><Month>01</Month><Day>25</Day></DateCompleted><DateRevised><Year>2009</Year><Month>10</Month><Day>6</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1521-4028</ISSN><JournalIssue CitedMedium="Internet"><Volume>49</Volume><Issue>5</Issue><PubDate><Year>2009</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of basic microbiology</Title><ISOAbbreviation>J. Basic Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Diversity of endophytic yeasts from sweet orange and their localization by scanning electron microscopy.</ArticleTitle><Pagination><MedlinePgn>441-51</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/jobm.200800328</ELocationID><Abstract><AbstractText>Endophytes are microorganisms that colonize plant tissues internally without causing harm to the host. Despite the increasing number of studies on sweet orange pathogens and endophytes, yeast has not been described as a sweet orange endophyte. In the present study, endophytic yeasts were isolated from sweet orange plants and identified by sequencing of internal transcribed spacer (ITS) rRNA. Plants sampled from four different sites in the state of São Paulo, Brazil exhibited different levels of CVC (citrus variegated chlorosis) development. Three citrus endophytic yeasts (CEYs), chosen as representative examples of the isolates observed, were identified as Rhodotorula mucilaginosa, Pichia guilliermondii and Cryptococcus flavescens. These strains were inoculated into axenic Citrus sinensis seedlings. After 45 days, endophytes were re-isolated in populations ranging from 10(6) to 10(9) CFU/g of plant tissue, but, in spite of the high concentrations of yeast cells, no disease symptoms were observed. Colonized plant material was examined by scanning electron microscopy (SEM), and yeast cells were found mainly in the stomata and xylem of plants, reinforcing their endophytic nature. P. guilliermondii was isolated primarily from plants colonized by the causal agent of CVC, Xylella fastidiosa. The supernatant from a culture of P. guilliermondii increased the in vitro growth of X. fastidiosa, suggesting that the yeast could assist in the establishment of this pathogen in its host plant and, therefore, contribute to the development of disease symptoms.</AbstractText><CopyrightInformation>Copyright 2009 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Gai</LastName><ForeName>Cláudia Santos</ForeName><Initials>CS</Initials><AffiliationInfo><Affiliation>Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, Brazil.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lacava</LastName><ForeName>Paulo Teixeira</ForeName><Initials>PT</Initials></Author><Author ValidYN="Y"><LastName>Maccheroni</LastName><ForeName>Walter</ForeName><Initials>W</Initials><Suffix>Jr</Suffix></Author><Author ValidYN="Y"><LastName>Glienke</LastName><ForeName>Chirlei</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Araújo</LastName><ForeName>Welington Luiz</ForeName><Initials>WL</Initials></Author><Author ValidYN="Y"><LastName>Miller</LastName><ForeName>Thomas Albert</ForeName><Initials>TA</Initials></Author><Author ValidYN="Y"><LastName>Azevedo</LastName><ForeName>João Lúcio</ForeName><Initials>JL</Initials></Author></AuthorList><Language>ENG</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>J Basic Microbiol</MedlineTA><NlmUniqueID>8503885</NlmUniqueID><ISSNLinking>0233-111X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021903">DNA, Ribosomal Spacer</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D001938" MajorTopicYN="N" Type="Geographic">Brazil</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032084" MajorTopicYN="N">Citrus sinensis</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003454" MajorTopicYN="N">Cryptococcus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021903" MajorTopicYN="N">DNA, Ribosomal Spacer</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D003627" MajorTopicYN="N">Data Interpretation, Statistical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017343" MajorTopicYN="N">Genes, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D054884" MajorTopicYN="N">Host-Pathogen Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008855" MajorTopicYN="N">Microscopy, Electron, Scanning</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010802" MajorTopicYN="N">Phylogeny</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010843" MajorTopicYN="N">Pichia</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant 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MajorTopicYN="N">ultrastructure</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D044167" MajorTopicYN="N">Xylella</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015003" MajorTopicYN="N">Yeasts</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000648" MajorTopicYN="Y">ultrastructure</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>10</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>10</Month><Day>3</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2010</Year><Month>1</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19798655</ArticleId><ArticleId IdType="doi">10.1002/jobm.200800328</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Brésil</li></country><region><li>État de São Paulo</li></region><settlement><li>São Paulo</li></settlement><orgName><li>Université de São Paulo</li></orgName></list><tree><noCountry><name sortKey="Araujo, Welington Luiz" sort="Araujo, Welington Luiz" uniqKey="Araujo W" first="Welington Luiz" last="Araújo">Welington Luiz Araújo</name><name sortKey="Azevedo, Joao Lucio" sort="Azevedo, Joao Lucio" uniqKey="Azevedo J" first="João Lúcio" last="Azevedo">João Lúcio Azevedo</name><name sortKey="Glienke, Chirlei" sort="Glienke, Chirlei" uniqKey="Glienke C" first="Chirlei" last="Glienke">Chirlei Glienke</name><name sortKey="Lacava, Paulo Teixeira" sort="Lacava, Paulo Teixeira" uniqKey="Lacava P" first="Paulo Teixeira" last="Lacava">Paulo Teixeira Lacava</name><name sortKey="Maccheroni, Walter" sort="Maccheroni, Walter" uniqKey="Maccheroni W" first="Walter" last="Maccheroni">Walter Maccheroni</name><name sortKey="Miller, Thomas Albert" sort="Miller, Thomas Albert" uniqKey="Miller T" first="Thomas Albert" last="Miller">Thomas Albert Miller</name></noCountry><country name="Brésil"><region name="État de São Paulo"><name sortKey="Gai, Claudia Santos" sort="Gai, Claudia Santos" uniqKey="Gai C" first="Cláudia Santos" last="Gai">Cláudia Santos Gai</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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