Analysis of orange juice for total cysteine and glutathione content by CZE with UV-absorption detection.
Identifieur interne : 000940 ( PubMed/Curation ); précédent : 000939; suivant : 000941Analysis of orange juice for total cysteine and glutathione content by CZE with UV-absorption detection.
Auteurs : Paweł Kubalczyk [Pologne] ; Edward BaldSource :
- Electrophoresis [ 1522-2683 ] ; 2009.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Cysteine, Glutathione.
- chemical , chemistry : Acetonitriles.
- analysis : Beverages.
- chemistry : Citrus sinensis, Fruit.
- methods : Electrophoresis, Capillary.
- Chromatography, High Pressure Liquid, Reproducibility of Results, Sensitivity and Specificity, Ultraviolet Rays.
Abstract
For total content of cysteine (Cys) and glutathione (GSH) a sample (500 microL) of neat orange juice or commercially available soft drinks is treated according to the procedure consisted of four essential steps: reduction of disulfide forms of the analytes to their thiol counterparts with Tris 2-carboxyethylphosphine, derivatization of thiols to their S-quinolinium derivatives with 2-chloro-1-methylquinolinium tetrafluroborate, separation of so-formed derivatives by capillary zone electrophoresis with ACN stacking, and detection and quantitation with the use of ultraviolet detector at 355 nm. Commercially available bare fused-silica capillary served as the CE column. The method is linear in a wide range of concentrations covering practical application. The coefficient of correlation between analyte concentrations within the range from 2.5 to 30.0 micromol/L and the detector response was for Cys and GSH 0.998 and 0.997, respectively. The imprecision for above-mentioned range was between 6.7 and 3.8, and 3.6 and 1.1% RSD for Cys and GSH, respectively. The method was applied to several samples of fresh juices and commercial orange beverages.
DOI: 10.1002/elps.200800741
PubMed: 19569125
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Commercially available bare fused-silica capillary served as the CE column. The method is linear in a wide range of concentrations covering practical application. The coefficient of correlation between analyte concentrations within the range from 2.5 to 30.0 micromol/L and the detector response was for Cys and GSH 0.998 and 0.997, respectively. The imprecision for above-mentioned range was between 6.7 and 3.8, and 3.6 and 1.1% RSD for Cys and GSH, respectively. The method was applied to several samples of fresh juices and commercial orange beverages.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19569125</PMID><DateCreated><Year>2009</Year><Month>7</Month><Day>27</Day></DateCreated><DateCompleted><Year>2009</Year><Month>11</Month><Day>10</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1522-2683</ISSN><JournalIssue CitedMedium="Internet"><Volume>30</Volume><Issue>13</Issue><PubDate><Year>2009</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Electrophoresis</Title><ISOAbbreviation>Electrophoresis</ISOAbbreviation></Journal><ArticleTitle>Analysis of orange juice for total cysteine and glutathione content by CZE with UV-absorption detection.</ArticleTitle><Pagination><MedlinePgn>2280-3</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/elps.200800741</ELocationID><Abstract><AbstractText>For total content of cysteine (Cys) and glutathione (GSH) a sample (500 microL) of neat orange juice or commercially available soft drinks is treated according to the procedure consisted of four essential steps: reduction of disulfide forms of the analytes to their thiol counterparts with Tris 2-carboxyethylphosphine, derivatization of thiols to their S-quinolinium derivatives with 2-chloro-1-methylquinolinium tetrafluroborate, separation of so-formed derivatives by capillary zone electrophoresis with ACN stacking, and detection and quantitation with the use of ultraviolet detector at 355 nm. Commercially available bare fused-silica capillary served as the CE column. The method is linear in a wide range of concentrations covering practical application. The coefficient of correlation between analyte concentrations within the range from 2.5 to 30.0 micromol/L and the detector response was for Cys and GSH 0.998 and 0.997, respectively. The imprecision for above-mentioned range was between 6.7 and 3.8, and 3.6 and 1.1% RSD for Cys and GSH, respectively. 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