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Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

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Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

Auteurs : Summar A. Naqvi [Pakistan] ; M. M. Khan [Pakistan] ; M. Shahid [Pakistan] ; M. J. Jaskani [Pakistan] ; Iqrar A. Khan [Pakistan] ; Mohammad Zuber [Pakistan] ; KHALID MAHMOOD ZIA [Pakistan]

Source :

Carbohydrate polymers [ 0144-8617 ] ; 2010.

RBID : Pascal:11-0033839

Descripteurs français

Pascal (Inist)
- Mucilage, Origine végétale, Porte greffe, Tégument, Graine, Citrus jambhiri, Citrus paradisi, Citrus sinensis, Composition chimique, Structure moléculaire, Dégradation enzymatique, Activité enzymatique, Cultivar, Etude comparative, Etude expérimentale, Composition en monosaccharides.

English descriptors

KwdEn :
- Chemical composition, Citrus jambhiri, Citrus paradisi, Citrus sinensis, Comparative study, Cultivar, Enzymatic activity, Enzymatic digestion, Experimental study, Molecular structure, Mucilage, Plant origin, Rootstock, Seeds, Tegument.

Abstract

Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg^-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g^-1 energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.

Affiliations:

Pakistan

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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chemical composition</term>
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<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Comparative study</term>
<term>Cultivar</term>
<term>Enzymatic activity</term>
<term>Enzymatic digestion</term>
<term>Experimental study</term>
<term>Molecular structure</term>
<term>Mucilage</term>
<term>Plant origin</term>
<term>Rootstock</term>
<term>Seeds</term>
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<term>Tégument</term>
<term>Graine</term>
<term>Citrus jambhiri</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Composition chimique</term>
<term>Structure moléculaire</term>
<term>Dégradation enzymatique</term>
<term>Activité enzymatique</term>
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<front><div type="abstract" xml:lang="en">Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg<sup>-1</sup>
 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g<sup>-1</sup>
 energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.</div>
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Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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