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Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

Identifieur interne : 000205 ( PascalFrancis/Corpus ); précédent : 000204; suivant : 000206

Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks

Auteurs : Summar A. Naqvi ; M. M. Khan ; M. Shahid ; M. J. Jaskani ; Iqrar A. Khan ; Mohammad Zuber ; KHALID MAHMOOD ZIA

Source :

RBID : Pascal:11-0033839

Descripteurs français

English descriptors

Abstract

Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g-1 energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 0144-8617
A02 01      @0 CAPOD8
A03   1    @0 Carbohydr. polym.
A05       @2 83
A06       @2 2
A08 01  1  ENG  @1 Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks
A11 01  1    @1 NAQVI (Summar A.)
A11 02  1    @1 KHAN (M. M.)
A11 03  1    @1 SHAHID (M.)
A11 04  1    @1 JASKANI (M. J.)
A11 05  1    @1 KHAN (Iqrar A.)
A11 06  1    @1 ZUBER (Mohammad)
A11 07  1    @1 KHALID MAHMOOD ZIA
A14 01      @1 Citrus Nursery Sanitation Laboratory, Institute of Horticultural Sciences, University of Agriculture @2 Faisalabad, 38040 @3 PAK @Z 1 aut. @Z 2 aut. @Z 4 aut. @Z 5 aut.
A14 02      @1 Biological Assay Section, Protein Molecular Biology Laboratory, Department of Chemistry and Biochemistry, University of Agriculture @2 Faisalabad, 38040 @3 PAK @Z 3 aut.
A14 03      @1 Department of Industrial Chemistry, GC University @2 Faisalabad, 38030 @3 PAK @Z 6 aut. @Z 7 aut.
A20       @1 623-628
A21       @1 2010
A23 01      @0 ENG
A43 01      @1 INIST @2 19272 @5 354000194501140410
A44       @0 0000 @1 © 2011 INIST-CNRS. All rights reserved.
A45       @0 1/2 p.
A47 01  1    @0 11-0033839
A60       @1 P
A61       @0 A
A64 01  1    @0 Carbohydrate polymers
A66 01      @0 GBR
C01 01    ENG  @0 Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g-1 energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.
C02 01  X    @0 001D09C04
C02 02  X    @0 002A35B09
C03 01  X  FRE  @0 Mucilage @5 02
C03 01  X  ENG  @0 Mucilage @5 02
C03 01  X  SPA  @0 Mucílago @5 02
C03 02  X  FRE  @0 Origine végétale @5 03
C03 02  X  ENG  @0 Plant origin @5 03
C03 02  X  SPA  @0 Origen vegetal @5 03
C03 03  X  FRE  @0 Porte greffe @5 04
C03 03  X  ENG  @0 Rootstock @5 04
C03 03  X  SPA  @0 Portainjerto @5 04
C03 04  X  FRE  @0 Tégument @5 05
C03 04  X  ENG  @0 Tegument @5 05
C03 04  X  SPA  @0 Tegumento @5 05
C03 05  X  FRE  @0 Graine @5 06
C03 05  X  ENG  @0 Seeds @5 06
C03 05  X  SPA  @0 Semillas @5 06
C03 06  X  FRE  @0 Citrus jambhiri @2 NS @5 07
C03 06  X  ENG  @0 Citrus jambhiri @2 NS @5 07
C03 06  X  SPA  @0 Citrus jambhiri @2 NS @5 07
C03 07  X  FRE  @0 Citrus paradisi @2 NS @5 08
C03 07  X  ENG  @0 Citrus paradisi @2 NS @5 08
C03 07  X  SPA  @0 Citrus paradisi @2 NS @5 08
C03 08  X  FRE  @0 Citrus sinensis @2 NS @5 09
C03 08  X  ENG  @0 Citrus sinensis @2 NS @5 09
C03 08  X  SPA  @0 Citrus sinensis @2 NS @5 09
C03 09  X  FRE  @0 Composition chimique @5 13
C03 09  X  ENG  @0 Chemical composition @5 13
C03 09  X  SPA  @0 Composición química @5 13
C03 10  X  FRE  @0 Structure moléculaire @5 14
C03 10  X  ENG  @0 Molecular structure @5 14
C03 10  X  SPA  @0 Estructura molecular @5 14
C03 11  X  FRE  @0 Dégradation enzymatique @5 15
C03 11  X  ENG  @0 Enzymatic digestion @5 15
C03 11  X  SPA  @0 Degradación enzimática @5 15
C03 12  X  FRE  @0 Activité enzymatique @5 17
C03 12  X  ENG  @0 Enzymatic activity @5 17
C03 12  X  SPA  @0 Actividad enzimática @5 17
C03 13  X  FRE  @0 Cultivar @5 18
C03 13  X  ENG  @0 Cultivar @5 18
C03 13  X  SPA  @0 Cultivar @5 18
C03 14  X  FRE  @0 Etude comparative @5 19
C03 14  X  ENG  @0 Comparative study @5 19
C03 14  X  SPA  @0 Estudio comparativo @5 19
C03 15  X  FRE  @0 Etude expérimentale @5 20
C03 15  X  ENG  @0 Experimental study @5 20
C03 15  X  SPA  @0 Estudio experimental @5 20
C03 16  X  FRE  @0 Composition en monosaccharides @4 INC @5 32
C07 01  X  FRE  @0 Oside polymère @2 NK @5 01
C07 01  X  ENG  @0 Oside polymer @2 NK @5 01
C07 01  X  SPA  @0 Osido polímero @2 NK @5 01
C07 02  X  FRE  @0 Rutaceae @2 NS
C07 02  X  ENG  @0 Rutaceae @2 NS
C07 02  X  SPA  @0 Rutaceae @2 NS
C07 03  X  FRE  @0 Dicotyledones @2 NS
C07 03  X  ENG  @0 Dicotyledones @2 NS
C07 03  X  SPA  @0 Dicotyledones @2 NS
C07 04  X  FRE  @0 Angiospermae @2 NS
C07 04  X  ENG  @0 Angiospermae @2 NS
C07 04  X  SPA  @0 Angiospermae @2 NS
C07 05  X  FRE  @0 Spermatophyta @2 NS
C07 05  X  ENG  @0 Spermatophyta @2 NS
C07 05  X  SPA  @0 Spermatophyta @2 NS
C07 06  X  FRE  @0 Agrume @5 10
C07 06  X  ENG  @0 Citrus fruit @5 10
C07 06  X  SPA  @0 Agrios @5 10
N21       @1 024
N44 01      @1 PSI
N82       @1 PSI

Format Inist (serveur)

NO : PASCAL 11-0033839 INIST
ET : Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks
AU : NAQVI (Summar A.); KHAN (M. M.); SHAHID (M.); JASKANI (M. J.); KHAN (Iqrar A.); ZUBER (Mohammad); KHALID MAHMOOD ZIA
AF : Citrus Nursery Sanitation Laboratory, Institute of Horticultural Sciences, University of Agriculture/Faisalabad, 38040/Pakistan (1 aut., 2 aut., 4 aut., 5 aut.); Biological Assay Section, Protein Molecular Biology Laboratory, Department of Chemistry and Biochemistry, University of Agriculture/Faisalabad, 38040/Pakistan (3 aut.); Department of Industrial Chemistry, GC University/Faisalabad, 38030/Pakistan (6 aut., 7 aut.)
DT : Publication en série; Niveau analytique
SO : Carbohydrate polymers; ISSN 0144-8617; Coden CAPOD8; Royaume-Uni; Da. 2010; Vol. 83; No. 2; Pp. 623-628; Bibl. 1/2 p.
LA : Anglais
EA : Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g-1 energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.
CC : 001D09C04; 002A35B09
FD : Mucilage; Origine végétale; Porte greffe; Tégument; Graine; Citrus jambhiri; Citrus paradisi; Citrus sinensis; Composition chimique; Structure moléculaire; Dégradation enzymatique; Activité enzymatique; Cultivar; Etude comparative; Etude expérimentale; Composition en monosaccharides
FG : Oside polymère; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume
ED : Mucilage; Plant origin; Rootstock; Tegument; Seeds; Citrus jambhiri; Citrus paradisi; Citrus sinensis; Chemical composition; Molecular structure; Enzymatic digestion; Enzymatic activity; Cultivar; Comparative study; Experimental study
EG : Oside polymer; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit
SD : Mucílago; Origen vegetal; Portainjerto; Tegumento; Semillas; Citrus jambhiri; Citrus paradisi; Citrus sinensis; Composición química; Estructura molecular; Degradación enzimática; Actividad enzimática; Cultivar; Estudio comparativo; Estudio experimental
LO : INIST-19272.354000194501140410
ID : 11-0033839

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Pascal:11-0033839

Le document en format XML

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<term>Citrus sinensis</term>
<term>Comparative study</term>
<term>Cultivar</term>
<term>Enzymatic activity</term>
<term>Enzymatic digestion</term>
<term>Experimental study</term>
<term>Molecular structure</term>
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<term>Citrus jambhiri</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Composition chimique</term>
<term>Structure moléculaire</term>
<term>Dégradation enzymatique</term>
<term>Activité enzymatique</term>
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<div type="abstract" xml:lang="en">Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg
<sup>-1</sup>
of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g
<sup>-1</sup>
energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.</div>
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</fA11>
<fA11 i1="04" i2="1">
<s1>JASKANI (M. J.)</s1>
</fA11>
<fA11 i1="05" i2="1">
<s1>KHAN (Iqrar A.)</s1>
</fA11>
<fA11 i1="06" i2="1">
<s1>ZUBER (Mohammad)</s1>
</fA11>
<fA11 i1="07" i2="1">
<s1>KHALID MAHMOOD ZIA</s1>
</fA11>
<fA14 i1="01">
<s1>Citrus Nursery Sanitation Laboratory, Institute of Horticultural Sciences, University of Agriculture</s1>
<s2>Faisalabad, 38040</s2>
<s3>PAK</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
<sZ>4 aut.</sZ>
<sZ>5 aut.</sZ>
</fA14>
<fA14 i1="02">
<s1>Biological Assay Section, Protein Molecular Biology Laboratory, Department of Chemistry and Biochemistry, University of Agriculture</s1>
<s2>Faisalabad, 38040</s2>
<s3>PAK</s3>
<sZ>3 aut.</sZ>
</fA14>
<fA14 i1="03">
<s1>Department of Industrial Chemistry, GC University</s1>
<s2>Faisalabad, 38030</s2>
<s3>PAK</s3>
<sZ>6 aut.</sZ>
<sZ>7 aut.</sZ>
</fA14>
<fA20>
<s1>623-628</s1>
</fA20>
<fA21>
<s1>2010</s1>
</fA21>
<fA23 i1="01">
<s0>ENG</s0>
</fA23>
<fA43 i1="01">
<s1>INIST</s1>
<s2>19272</s2>
<s5>354000194501140410</s5>
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<fA44>
<s0>0000</s0>
<s1>© 2011 INIST-CNRS. All rights reserved.</s1>
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<s0>1/2 p.</s0>
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<fA47 i1="01" i2="1">
<s0>11-0033839</s0>
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<fA60>
<s1>P</s1>
</fA60>
<fA61>
<s0>A</s0>
</fA61>
<fA64 i1="01" i2="1">
<s0>Carbohydrate polymers</s0>
</fA64>
<fA66 i1="01">
<s0>GBR</s0>
</fA66>
<fC01 i1="01" l="ENG">
<s0>Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg
<sup>-1</sup>
of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g
<sup>-1</sup>
energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.</s0>
</fC01>
<fC02 i1="01" i2="X">
<s0>001D09C04</s0>
</fC02>
<fC02 i1="02" i2="X">
<s0>002A35B09</s0>
</fC02>
<fC03 i1="01" i2="X" l="FRE">
<s0>Mucilage</s0>
<s5>02</s5>
</fC03>
<fC03 i1="01" i2="X" l="ENG">
<s0>Mucilage</s0>
<s5>02</s5>
</fC03>
<fC03 i1="01" i2="X" l="SPA">
<s0>Mucílago</s0>
<s5>02</s5>
</fC03>
<fC03 i1="02" i2="X" l="FRE">
<s0>Origine végétale</s0>
<s5>03</s5>
</fC03>
<fC03 i1="02" i2="X" l="ENG">
<s0>Plant origin</s0>
<s5>03</s5>
</fC03>
<fC03 i1="02" i2="X" l="SPA">
<s0>Origen vegetal</s0>
<s5>03</s5>
</fC03>
<fC03 i1="03" i2="X" l="FRE">
<s0>Porte greffe</s0>
<s5>04</s5>
</fC03>
<fC03 i1="03" i2="X" l="ENG">
<s0>Rootstock</s0>
<s5>04</s5>
</fC03>
<fC03 i1="03" i2="X" l="SPA">
<s0>Portainjerto</s0>
<s5>04</s5>
</fC03>
<fC03 i1="04" i2="X" l="FRE">
<s0>Tégument</s0>
<s5>05</s5>
</fC03>
<fC03 i1="04" i2="X" l="ENG">
<s0>Tegument</s0>
<s5>05</s5>
</fC03>
<fC03 i1="04" i2="X" l="SPA">
<s0>Tegumento</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="FRE">
<s0>Graine</s0>
<s5>06</s5>
</fC03>
<fC03 i1="05" i2="X" l="ENG">
<s0>Seeds</s0>
<s5>06</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Semillas</s0>
<s5>06</s5>
</fC03>
<fC03 i1="06" i2="X" l="FRE">
<s0>Citrus jambhiri</s0>
<s2>NS</s2>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="ENG">
<s0>Citrus jambhiri</s0>
<s2>NS</s2>
<s5>07</s5>
</fC03>
<fC03 i1="06" i2="X" l="SPA">
<s0>Citrus jambhiri</s0>
<s2>NS</s2>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="FRE">
<s0>Citrus paradisi</s0>
<s2>NS</s2>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG">
<s0>Citrus paradisi</s0>
<s2>NS</s2>
<s5>08</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA">
<s0>Citrus paradisi</s0>
<s2>NS</s2>
<s5>08</s5>
</fC03>
<fC03 i1="08" i2="X" l="FRE">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="ENG">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>09</s5>
</fC03>
<fC03 i1="08" i2="X" l="SPA">
<s0>Citrus sinensis</s0>
<s2>NS</s2>
<s5>09</s5>
</fC03>
<fC03 i1="09" i2="X" l="FRE">
<s0>Composition chimique</s0>
<s5>13</s5>
</fC03>
<fC03 i1="09" i2="X" l="ENG">
<s0>Chemical composition</s0>
<s5>13</s5>
</fC03>
<fC03 i1="09" i2="X" l="SPA">
<s0>Composición química</s0>
<s5>13</s5>
</fC03>
<fC03 i1="10" i2="X" l="FRE">
<s0>Structure moléculaire</s0>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="ENG">
<s0>Molecular structure</s0>
<s5>14</s5>
</fC03>
<fC03 i1="10" i2="X" l="SPA">
<s0>Estructura molecular</s0>
<s5>14</s5>
</fC03>
<fC03 i1="11" i2="X" l="FRE">
<s0>Dégradation enzymatique</s0>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="ENG">
<s0>Enzymatic digestion</s0>
<s5>15</s5>
</fC03>
<fC03 i1="11" i2="X" l="SPA">
<s0>Degradación enzimática</s0>
<s5>15</s5>
</fC03>
<fC03 i1="12" i2="X" l="FRE">
<s0>Activité enzymatique</s0>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG">
<s0>Enzymatic activity</s0>
<s5>17</s5>
</fC03>
<fC03 i1="12" i2="X" l="SPA">
<s0>Actividad enzimática</s0>
<s5>17</s5>
</fC03>
<fC03 i1="13" i2="X" l="FRE">
<s0>Cultivar</s0>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="ENG">
<s0>Cultivar</s0>
<s5>18</s5>
</fC03>
<fC03 i1="13" i2="X" l="SPA">
<s0>Cultivar</s0>
<s5>18</s5>
</fC03>
<fC03 i1="14" i2="X" l="FRE">
<s0>Etude comparative</s0>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="ENG">
<s0>Comparative study</s0>
<s5>19</s5>
</fC03>
<fC03 i1="14" i2="X" l="SPA">
<s0>Estudio comparativo</s0>
<s5>19</s5>
</fC03>
<fC03 i1="15" i2="X" l="FRE">
<s0>Etude expérimentale</s0>
<s5>20</s5>
</fC03>
<fC03 i1="15" i2="X" l="ENG">
<s0>Experimental study</s0>
<s5>20</s5>
</fC03>
<fC03 i1="15" i2="X" l="SPA">
<s0>Estudio experimental</s0>
<s5>20</s5>
</fC03>
<fC03 i1="16" i2="X" l="FRE">
<s0>Composition en monosaccharides</s0>
<s4>INC</s4>
<s5>32</s5>
</fC03>
<fC07 i1="01" i2="X" l="FRE">
<s0>Oside polymère</s0>
<s2>NK</s2>
<s5>01</s5>
</fC07>
<fC07 i1="01" i2="X" l="ENG">
<s0>Oside polymer</s0>
<s2>NK</s2>
<s5>01</s5>
</fC07>
<fC07 i1="01" i2="X" l="SPA">
<s0>Osido polímero</s0>
<s2>NK</s2>
<s5>01</s5>
</fC07>
<fC07 i1="02" i2="X" l="FRE">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="ENG">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Rutaceae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="FRE">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="06" i2="X" l="FRE">
<s0>Agrume</s0>
<s5>10</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG">
<s0>Citrus fruit</s0>
<s5>10</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA">
<s0>Agrios</s0>
<s5>10</s5>
</fC07>
<fN21>
<s1>024</s1>
</fN21>
<fN44 i1="01">
<s1>PSI</s1>
</fN44>
<fN82>
<s1>PSI</s1>
</fN82>
</pA>
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<server>
<NO>PASCAL 11-0033839 INIST</NO>
<ET>Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks</ET>
<AU>NAQVI (Summar A.); KHAN (M. M.); SHAHID (M.); JASKANI (M. J.); KHAN (Iqrar A.); ZUBER (Mohammad); KHALID MAHMOOD ZIA</AU>
<AF>Citrus Nursery Sanitation Laboratory, Institute of Horticultural Sciences, University of Agriculture/Faisalabad, 38040/Pakistan (1 aut., 2 aut., 4 aut., 5 aut.); Biological Assay Section, Protein Molecular Biology Laboratory, Department of Chemistry and Biochemistry, University of Agriculture/Faisalabad, 38040/Pakistan (3 aut.); Department of Industrial Chemistry, GC University/Faisalabad, 38030/Pakistan (6 aut., 7 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Carbohydrate polymers; ISSN 0144-8617; Coden CAPOD8; Royaume-Uni; Da. 2010; Vol. 83; No. 2; Pp. 623-628; Bibl. 1/2 p.</SO>
<LA>Anglais</LA>
<EA>Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg
<sup>-1</sup>
of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g
<sup>-1</sup>
energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.</EA>
<CC>001D09C04; 002A35B09</CC>
<FD>Mucilage; Origine végétale; Porte greffe; Tégument; Graine; Citrus jambhiri; Citrus paradisi; Citrus sinensis; Composition chimique; Structure moléculaire; Dégradation enzymatique; Activité enzymatique; Cultivar; Etude comparative; Etude expérimentale; Composition en monosaccharides</FD>
<FG>Oside polymère; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume</FG>
<ED>Mucilage; Plant origin; Rootstock; Tegument; Seeds; Citrus jambhiri; Citrus paradisi; Citrus sinensis; Chemical composition; Molecular structure; Enzymatic digestion; Enzymatic activity; Cultivar; Comparative study; Experimental study</ED>
<EG>Oside polymer; Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit</EG>
<SD>Mucílago; Origen vegetal; Portainjerto; Tegumento; Semillas; Citrus jambhiri; Citrus paradisi; Citrus sinensis; Composición química; Estructura molecular; Degradación enzimática; Actividad enzimática; Cultivar; Estudio comparativo; Estudio experimental</SD>
<LO>INIST-19272.354000194501140410</LO>
<ID>11-0033839</ID>
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