Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks
Identifieur interne : 001D37 ( Main/Merge ); précédent : 001D36; suivant : 001D38Biochemical profiling of mucilage extracted from seeds of different citrus rootstocks
Auteurs : Summar A. Naqvi [Pakistan] ; M. M. Khan [Pakistan] ; M. Shahid [Pakistan] ; M. J. Jaskani [Pakistan] ; Iqrar A. Khan [Pakistan] ; Mohammad Zuber [Pakistan] ; KHALID MAHMOOD ZIA [Pakistan]Source :
- Carbohydrate polymers [ 0144-8617 ] ; 2010.
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Abstract
Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg-1 of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g-1 energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Chemical composition</term>
<term>Citrus jambhiri</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Comparative study</term>
<term>Cultivar</term>
<term>Enzymatic activity</term>
<term>Enzymatic digestion</term>
<term>Experimental study</term>
<term>Molecular structure</term>
<term>Mucilage</term>
<term>Plant origin</term>
<term>Rootstock</term>
<term>Seeds</term>
<term>Tegument</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Mucilage</term>
<term>Origine végétale</term>
<term>Porte greffe</term>
<term>Tégument</term>
<term>Graine</term>
<term>Citrus jambhiri</term>
<term>Citrus paradisi</term>
<term>Citrus sinensis</term>
<term>Composition chimique</term>
<term>Structure moléculaire</term>
<term>Dégradation enzymatique</term>
<term>Activité enzymatique</term>
<term>Cultivar</term>
<term>Etude comparative</term>
<term>Etude expérimentale</term>
<term>Composition en monosaccharides</term>
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<front><div type="abstract" xml:lang="en">Hetero-polysaccharide mucilage was extracted from the seed coats of different citrus rootstocks viz. Rough lemon, Sachtion citrumelo and Yuma citrange for investigating its biochemical and molecular properties. Investigations showed that the mucilage contained (mg/g) starch 3.13-5.04: maltose 3.23-4.31; glucosamine 0.017-0.289; D-xylose 0.059-0.107 and total soluble sugars 8.13-11.82. Specific enzyme activities were 16.98-35.96, 30.60-98.45, 42.00-73.98, 660.98-738.35 and 7.660-19.27 IU mg<sup>-1</sup>
of protein for protease, amylase, catalase, peroxidase and superoxide dismutase, respectively. Proximate analysis showed 12.85-13.94% moisture, 11.25-14.06% crude protein, 0.31-0.86% crude lipid, 1.3 -2.69% crude fibre, 2.95-3.45% ash and 81.48-91.49 kJ 100 g<sup>-1</sup>
energy. The comparative characterizatio of the extractable proteins was profiled by SDS-PAGE and quantified using Bradford assay. Structural properties of samples were analyzed and compared using Fourier transformation infrared (FT-IR) spectroscopy.</div>
</front>
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<name sortKey="Jaskani, M J" sort="Jaskani, M J" uniqKey="Jaskani M" first="M. J." last="Jaskani">M. J. Jaskani</name>
<name sortKey="Khalid Mahmood Zia" sort="Khalid Mahmood Zia" uniqKey="Khalid Mahmood Zia" last="Khalid Mahmood Zia">KHALID MAHMOOD ZIA</name>
<name sortKey="Khan, Iqrar A" sort="Khan, Iqrar A" uniqKey="Khan I" first="Iqrar A." last="Khan">Iqrar A. Khan</name>
<name sortKey="Khan, M M" sort="Khan, M M" uniqKey="Khan M" first="M. M." last="Khan">M. M. Khan</name>
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