OrangerV1, Main, Exploration, bibRecord, 002653

Gene isolation, analysis of expression, and in vitro synthesis of glutathione S-transferase from orange fruit [Citrus sinensis L. (Osbeck)]

Identifieur interne : 002653 ( Main/Exploration ); précédent : 002652; suivant : 002654

Gene isolation, analysis of expression, and in vitro synthesis of glutathione S-transferase from orange fruit [Citrus sinensis L. (Osbeck)]

Auteurs : Angela Roberta Lo Piero [Italie] ; Ivana Puglisi [Italie] ; Goffredo Petrone [Italie]

Source :

Journal of agricultural and food chemistry : (Print) [ 0021-8561 ] ; 2006.

RBID : Pascal:07-0005154

Descripteurs français

Pascal (Inist)
- Expression génique, Isolement, In vitro, Citrus sinensis, Synthèse, Glutathione transferase, Orange, Agrume, Flavonoïde, Anthocyane, Sang, Froid, Stress, Temps réel, Réaction chaîne polymérase.
Wicri :
- topic : Agrume.

English descriptors

KwdEn :
- Amino Acid Sequence, Anthocyanin, Blood, Citrus fruit, Citrus sinensis, Citrus sinensis (enzymology), Cloning, Molecular, Cold, DNA, Complementary (analysis), Flavonoid, Fruit, Gene Expression Regulation, Plant, Gene expression, Glutathione Transferase (biosynthesis), Glutathione Transferase (genetics), Glutathione transferase, Humans, In vitro, Isolation, Molecular Sequence Data, Orange, Polymerase chain reaction, Real time, Stress, Synthesis.
MESH :
- chemical , analysis : DNA, Complementary.
- chemical , biosynthesis : Glutathione Transferase.
- enzymology : Citrus sinensis.
- chemical , genetics : Glutathione Transferase.
- Amino Acid Sequence, Cloning, Molecular, Fruit, Gene Expression Regulation, Plant, Humans, Molecular Sequence Data.

Abstract

Glutathione S-transferases (GSTs) (EC 2.5.1.18) are ubiquitous enzymes that have a defined role in xenobiotic detoxification, but a deeper knowledge of their function in endogenous metabolism is still lacking. In this work, we isolated the cDNAs as well as the genomic clones of orange GSTs. Having considered gene organization and homology data, we suggest that the isolated GSTgene is probably involved in the vacuolar import of anthocyanins. We also found that the blood and blond orange GSTs shared the same nucleotide sequences, but as expected, the GST expression in the nonpigmented orange cultivar [Citrus sinensis L. (Osbeck)] (Navel and Ovale) was strongly reduced as compared to that of the pigmented orange (Tarocco). Interestingly, in the crude extracts of pigmented orange fruits, the GSTactivity was reproducibly detected by providing either 1-chloro-2,4 dinitrobenzene (CDNB) or cyanidin-3-O-glucoside (C-3-G) as substrates; moreover, we have shown that cyanidin-3-O-glucoside acted as a powerful competitive inhibitor of 1-chloro-2,4 dinitrobenzene conjugation to reduced glutathione (GSH) in the pigmented orange, confirming that this molecule might easily bind to the active site of the enzyme and functions as a putative substrate. In addition, we have reported here the successful in vitro expression of orange GSTcDNAs leading to a GST enzyme that is active against cyanidin-3-O-glucoside, thus suggesting the probable involvement of the isolated gene in the tagging of anthocyanins for vacuolar import. This last result will help to study the kinetic and structural properties of orange fruit GSTavoiding time-consuming protein purification procedures.

Affiliations:

Italie

Le document en format XML

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<front><div type="abstract" xml:lang="en">Glutathione S-transferases (GSTs) (EC 2.5.1.18) are ubiquitous enzymes that have a defined role in xenobiotic detoxification, but a deeper knowledge of their function in endogenous metabolism is still lacking. In this work, we isolated the cDNAs as well as the genomic clones of orange GSTs. Having considered gene organization and homology data, we suggest that the isolated GSTgene is probably involved in the vacuolar import of anthocyanins. We also found that the blood and blond orange GSTs shared the same nucleotide sequences, but as expected, the GST expression in the nonpigmented orange cultivar [Citrus sinensis L. (Osbeck)] (Navel and Ovale) was strongly reduced as compared to that of the pigmented orange (Tarocco). Interestingly, in the crude extracts of pigmented orange fruits, the GSTactivity was reproducibly detected by providing either 1-chloro-2,4 dinitrobenzene (CDNB) or cyanidin-3-O-glucoside (C-3-G) as substrates; moreover, we have shown that cyanidin-3-O-glucoside acted as a powerful competitive inhibitor of 1-chloro-2,4 dinitrobenzene conjugation to reduced glutathione (GSH) in the pigmented orange, confirming that this molecule might easily bind to the active site of the enzyme and functions as a putative substrate. In addition, we have reported here the successful in vitro expression of orange GSTcDNAs leading to a GST enzyme that is active against cyanidin-3-O-glucoside, thus suggesting the probable involvement of the isolated gene in the tagging of anthocyanins for vacuolar import. This last result will help to study the kinetic and structural properties of orange fruit GSTavoiding time-consuming protein purification procedures.</div>
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Gene isolation, analysis of expression, and in vitro synthesis of glutathione S-transferase from orange fruit [Citrus sinensis L. (Osbeck)]

Gene isolation, analysis of expression, and in vitro synthesis of glutathione S-transferase from orange fruit [Citrus sinensis L. (Osbeck)]

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

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