Simultaneous analysis of serotonin, melatonin, piceid and resveratrol in fruits using liquid chromatography tandem mass spectrometry.
Identifieur interne : 001656 ( Main/Curation ); précédent : 001655; suivant : 001657Simultaneous analysis of serotonin, melatonin, piceid and resveratrol in fruits using liquid chromatography tandem mass spectrometry.
Auteurs : Xin Huang [Canada] ; Giuseppe MazzaSource :
- Journal of chromatography. A [ 1873-3778 ] ; 2011.
English descriptors
- KwdEn :
- Chromatography, High Pressure Liquid (methods), Citrus sinensis (chemistry), Fruit (chemistry), Glucosides (analysis), Glucosides (chemistry), Melatonin (analysis), Reproducibility of Results, Sensitivity and Specificity, Serotonin (analysis), Stilbenes (analysis), Stilbenes (chemistry), Tandem Mass Spectrometry (methods), Vitis (chemistry).
- MESH :
- chemical , analysis : Glucosides, Melatonin, Serotonin, Stilbenes.
- chemistry : Citrus sinensis, Fruit, Glucosides, Stilbenes, Vitis.
- methods : Chromatography, High Pressure Liquid, Tandem Mass Spectrometry.
- Reproducibility of Results, Sensitivity and Specificity.
Abstract
An analytical method was developed for the simultaneous quantification of serotonin, melatonin, trans- and cis-piceid, and trans- and cis-resveratrol using reversed-phase high performance liquid chromatography coupled to mass spectrometry (HPLC-MS) with electrospray ionization (ESI) in both positive and negative ionization modes. HPLC optimal analytical separation was achieved using a mixture of acetonitrile and water with 0.1% formic acid as the mobile phase in linear gradient elution. The mass spectrometry parameters were optimized for reliable quantification and the enhanced selectivity and sensitivity selected reaction monitoring mode (SRM) was applied. For extraction, the direct analysis of initial methanol extracts was compared with further ethyl acetate extraction. In order to demonstrate the applicability of this analytical method, serotonin, melatonin, trans- and cis-piceid, and trans- and cis-resveratrol from 24 kinds of commonly consumed fruits were quantified. The highest serotonin content was found in plantain, while orange bell peppers had the highest melatonin content. Grape samples possessed higher trans- and cis-piceid, and trans- and cis-resveratrol contents than the other fruits. The results indicate that the combination of HPLC-MS detection and simple sample preparation allows the rapid and accurate quantification of serotonin, melatonin, trans- and cis-piceid, and trans- and cis-resveratrol in fruits.
DOI: 10.1016/j.chroma.2011.04.049
PubMed: 21570690
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pubmed:21570690Le document en format XML
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<author><name sortKey="Huang, Xin" sort="Huang, Xin" uniqKey="Huang X" first="Xin" last="Huang">Xin Huang</name>
<affiliation wicri:level="1"><nlm:affiliation>Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada.</nlm:affiliation>
<country xml:lang="fr">Canada</country>
<wicri:regionArea>Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia</wicri:regionArea>
<wicri:noRegion>British Columbia</wicri:noRegion>
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<author><name sortKey="Mazza, Giuseppe" sort="Mazza, Giuseppe" uniqKey="Mazza G" first="Giuseppe" last="Mazza">Giuseppe Mazza</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Simultaneous analysis of serotonin, melatonin, piceid and resveratrol in fruits using liquid chromatography tandem mass spectrometry.</title>
<author><name sortKey="Huang, Xin" sort="Huang, Xin" uniqKey="Huang X" first="Xin" last="Huang">Xin Huang</name>
<affiliation wicri:level="1"><nlm:affiliation>Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, British Columbia, Canada.</nlm:affiliation>
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<series><title level="j">Journal of chromatography. A</title>
<idno type="eISSN">1873-3778</idno>
<imprint><date when="2011" type="published">2011</date>
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<term>Citrus sinensis (chemistry)</term>
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<term>Glucosides (analysis)</term>
<term>Glucosides (chemistry)</term>
<term>Melatonin (analysis)</term>
<term>Reproducibility of Results</term>
<term>Sensitivity and Specificity</term>
<term>Serotonin (analysis)</term>
<term>Stilbenes (analysis)</term>
<term>Stilbenes (chemistry)</term>
<term>Tandem Mass Spectrometry (methods)</term>
<term>Vitis (chemistry)</term>
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<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Glucosides</term>
<term>Melatonin</term>
<term>Serotonin</term>
<term>Stilbenes</term>
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<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus sinensis</term>
<term>Fruit</term>
<term>Glucosides</term>
<term>Stilbenes</term>
<term>Vitis</term>
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<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term>
<term>Tandem Mass Spectrometry</term>
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<front><div type="abstract" xml:lang="en">An analytical method was developed for the simultaneous quantification of serotonin, melatonin, trans- and cis-piceid, and trans- and cis-resveratrol using reversed-phase high performance liquid chromatography coupled to mass spectrometry (HPLC-MS) with electrospray ionization (ESI) in both positive and negative ionization modes. HPLC optimal analytical separation was achieved using a mixture of acetonitrile and water with 0.1% formic acid as the mobile phase in linear gradient elution. The mass spectrometry parameters were optimized for reliable quantification and the enhanced selectivity and sensitivity selected reaction monitoring mode (SRM) was applied. For extraction, the direct analysis of initial methanol extracts was compared with further ethyl acetate extraction. In order to demonstrate the applicability of this analytical method, serotonin, melatonin, trans- and cis-piceid, and trans- and cis-resveratrol from 24 kinds of commonly consumed fruits were quantified. The highest serotonin content was found in plantain, while orange bell peppers had the highest melatonin content. Grape samples possessed higher trans- and cis-piceid, and trans- and cis-resveratrol contents than the other fruits. The results indicate that the combination of HPLC-MS detection and simple sample preparation allows the rapid and accurate quantification of serotonin, melatonin, trans- and cis-piceid, and trans- and cis-resveratrol in fruits.</div>
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