AustralieFrV1, Ncbi, Curation, bibRecord, 002F07

Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora

Identifieur interne : 002F07 ( Ncbi/Curation ); précédent : 002F06; suivant : 002F08

Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora

Auteurs : Kimberley A. Lema [Australie, France] ; Peta L. Clode [Australie] ; Matt R. Kilburn [Australie] ; Ruth Thornton [Australie] ; Bette L. Willis [Australie] ; David G. Bourne [Australie]

Source :

The ISME Journal [ 1751-7362 ] ; 2015.

RBID : PMC:4918436

Descripteurs français

KwdFr :
- Animaux, Anthozoa (microbiologie), Azote (métabolisme), Bactéries fixatrices d'azote (métabolisme), Fixation de l'azote, Larve, Technique FISH.
MESH :
- microbiologie : Anthozoa.
- métabolisme : Azote, Bactéries fixatrices d'azote.
- Animaux, Fixation de l'azote, Larve, Technique FISH.

English descriptors

KwdEn :
- Animals, Anthozoa (microbiology), In Situ Hybridization, Fluorescence, Larva, Nitrogen (metabolism), Nitrogen Fixation, Nitrogen-Fixing Bacteria (metabolism).
MESH :
- chemical , metabolism : Nitrogen.
- metabolism : Nitrogen-Fixing Bacteria.
- microbiology : Anthozoa.
- Animals, In Situ Hybridization, Fluorescence, Larva, Nitrogen Fixation.

Abstract

Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N₂) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of ¹⁵N-enriched diazotrophic Vibrio sp. isolated from Acropora millepora into conspecific coral larvae. Incorporation of Vibrio sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where Vibrio cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918436

DOI: 10.1038/ismej.2015.229
PubMed: 26696324
PubMed Central: 4918436

Links toward previous steps (curation, corpus...)

to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000674
to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000673
to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000F26
to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001C75
to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001C51
to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001C51
to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :002F07

Links to Exploration step

PMC:4918436

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral <italic>Acropora millepora</italic>
</title>
<author><name sortKey="Lema, Kimberley A" sort="Lema, Kimberley A" uniqKey="Lema K" first="Kimberley A" last="Lema">Kimberley A. Lema</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff4"><institution>IFREMER, Dyneco Pelagos</institution>
, Plouzané,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Clode, Peta L" sort="Clode, Peta L" uniqKey="Clode P" first="Peta L" last="Clode">Peta L. Clode</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff6"><institution>The Oceans Institute, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kilburn, Matt R" sort="Kilburn, Matt R" uniqKey="Kilburn M" first="Matt R" last="Kilburn">Matt R. Kilburn</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Thornton, Ruth" sort="Thornton, Ruth" uniqKey="Thornton R" first="Ruth" last="Thornton">Ruth Thornton</name>
<affiliation wicri:level="1"><nlm:aff id="aff7"><institution>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Willis, Bette L" sort="Willis, Bette L" uniqKey="Willis B" first="Bette L" last="Willis">Bette L. Willis</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bourne, David G" sort="Bourne, David G" uniqKey="Bourne D" first="David G" last="Bourne">David G. Bourne</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">26696324</idno>
<idno type="pmc">4918436</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4918436</idno>
<idno type="RBID">PMC:4918436</idno>
<idno type="doi">10.1038/ismej.2015.229</idno>
<date when="2015">2015</date>
<idno type="wicri:Area/Pmc/Corpus">000674</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000674</idno>
<idno type="wicri:Area/Pmc/Curation">000673</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000673</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000F26</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000F26</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="wicri:Area/PubMed/Corpus">001C75</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001C75</idno>
<idno type="wicri:Area/PubMed/Curation">001C51</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001C51</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001C51</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001C51</idno>
<idno type="wicri:Area/Ncbi/Merge">002F07</idno>
<idno type="wicri:Area/Ncbi/Curation">002F07</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral <italic>Acropora millepora</italic>
</title>
<author><name sortKey="Lema, Kimberley A" sort="Lema, Kimberley A" uniqKey="Lema K" first="Kimberley A" last="Lema">Kimberley A. Lema</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff4"><institution>IFREMER, Dyneco Pelagos</institution>
, Plouzané,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Clode, Peta L" sort="Clode, Peta L" uniqKey="Clode P" first="Peta L" last="Clode">Peta L. Clode</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff6"><institution>The Oceans Institute, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kilburn, Matt R" sort="Kilburn, Matt R" uniqKey="Kilburn M" first="Matt R" last="Kilburn">Matt R. Kilburn</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Thornton, Ruth" sort="Thornton, Ruth" uniqKey="Thornton R" first="Ruth" last="Thornton">Ruth Thornton</name>
<affiliation wicri:level="1"><nlm:aff id="aff7"><institution>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Willis, Bette L" sort="Willis, Bette L" uniqKey="Willis B" first="Bette L" last="Willis">Bette L. Willis</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bourne, David G" sort="Bourne, David G" uniqKey="Bourne D" first="David G" last="Bourne">David G. Bourne</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">The ISME Journal</title>
<idno type="ISSN">1751-7362</idno>
<idno type="eISSN">1751-7370</idno>
<imprint><date when="2015">2015</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Anthozoa (microbiology)</term>
<term>In Situ Hybridization, Fluorescence</term>
<term>Larva</term>
<term>Nitrogen (metabolism)</term>
<term>Nitrogen Fixation</term>
<term>Nitrogen-Fixing Bacteria (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anthozoa (microbiologie)</term>
<term>Azote (métabolisme)</term>
<term>Bactéries fixatrices d'azote (métabolisme)</term>
<term>Fixation de l'azote</term>
<term>Larve</term>
<term>Technique FISH</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrogen</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nitrogen-Fixing Bacteria</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Anthozoa</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Anthozoa</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term>
<term>Bactéries fixatrices d'azote</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>In Situ Hybridization, Fluorescence</term>
<term>Larva</term>
<term>Nitrogen Fixation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Fixation de l'azote</term>
<term>Larve</term>
<term>Technique FISH</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N<sub>2</sub>
) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence <italic>in situ</italic>
 hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of <sup>15</sup>
N-enriched diazotrophic <italic>Vibrio</italic>
 sp. isolated from <italic>Acropora millepora</italic>
 into conspecific coral larvae. Incorporation of <italic>Vibrio</italic>
 sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where <italic>Vibrio</italic>
 cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.</p>
</div>
</front>
</TEI>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Ncbi/Curation

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002F07 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 002F07 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Asie
   |area=    AustralieFrV1
   |flux=    Ncbi
   |étape=   Curation
   |type=    RBID
   |clé=     PMC:4918436
   |texte=   Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i   -Sk "pubmed:26696324" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd   \
       | NlmPubMed2Wicri -a AustralieFrV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Dec 5 10:43:12 2017. Site generation: Tue Mar 5 14:07:20 2024

	Serveur d'exploration sur les relations entre la France et l'Australie
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur les relations entre la France et l'Australie

Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora

Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki