Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora
Identifieur interne : 000673 ( Pmc/Curation ); précédent : 000672; suivant : 000674Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora
Auteurs : Kimberley A. Lema [Australie, France] ; Peta L. Clode [Australie] ; Matt R. Kilburn [Australie] ; Ruth Thornton [Australie] ; Bette L. Willis [Australie] ; David G. Bourne [Australie]Source :
- The ISME Journal [ 1751-7362 ] ; 2015.
Abstract
Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N2) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence
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DOI: 10.1038/ismej.2015.229
PubMed: 26696324
PubMed Central: 4918436
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Lema</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University</institution>, Townsville, Queensland,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC</institution>, Townsville, Queensland,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>, Townsville, Queensland,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"><institution>IFREMER, Dyneco Pelagos</institution>, Plouzané,<country>France</country></nlm:aff><country xml:lang="fr">France</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Clode, Peta L" sort="Clode, Peta L" uniqKey="Clode P" first="Peta L" last="Clode">Peta L. Clode</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy</institution>, Crawley, Western Australia,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6"><institution>The Oceans Institute, The University of Western Australia, Stirling Hwy</institution>, Crawley, Western Australia,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Kilburn, Matt R" sort="Kilburn, Matt R" uniqKey="Kilburn M" first="Matt R" last="Kilburn">Matt R. Kilburn</name><affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy</institution>, Crawley, Western Australia,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Thornton, Ruth" sort="Thornton, Ruth" uniqKey="Thornton R" first="Ruth" last="Thornton">Ruth Thornton</name><affiliation wicri:level="1"><nlm:aff id="aff7"><institution>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy</institution>, Crawley, Western Australia,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Willis, Bette L" sort="Willis, Bette L" uniqKey="Willis B" first="Bette L" last="Willis">Bette L. Willis</name><affiliation wicri:level="1"><nlm:aff id="aff1"><institution>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University</institution>, Townsville, Queensland,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>, Townsville, Queensland,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author><author><name sortKey="Bourne, David G" sort="Bourne, David G" uniqKey="Bourne D" first="David G" last="Bourne">David G. Bourne</name><affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC</institution>, Townsville, Queensland,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>, Townsville, Queensland,<country>Australia</country></nlm:aff><country xml:lang="fr">Australie</country><wicri:regionArea># see nlm:aff country strict</wicri:regionArea></affiliation></author></analytic><series><title level="j">The ISME Journal</title><idno type="ISSN">1751-7362</idno><idno type="eISSN">1751-7370</idno><imprint><date when="2015">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N<sub>2</sub>) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence <italic>in situ</italic> hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of <sup>15</sup>N-enriched diazotrophic <italic>Vibrio</italic> sp. isolated from <italic>Acropora millepora</italic> into conspecific coral larvae. Incorporation of <italic>Vibrio</italic> sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where <italic>Vibrio</italic> cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.</p></div></front></TEI><pmc article-type="letter"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
<front><journal-meta><journal-id journal-id-type="nlm-ta">ISME J</journal-id><journal-id journal-id-type="iso-abbrev">ISME J</journal-id><journal-title-group><journal-title>The ISME Journal</journal-title></journal-title-group><issn pub-type="ppub">1751-7362</issn><issn pub-type="epub">1751-7370</issn><publisher><publisher-name>Nature Publishing Group</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">26696324</article-id><article-id pub-id-type="pmc">4918436</article-id><article-id pub-id-type="pii">ismej2015229</article-id><article-id pub-id-type="doi">10.1038/ismej.2015.229</article-id><article-categories><subj-group subj-group-type="heading"><subject>Short Communication</subject></subj-group></article-categories><title-group><article-title>Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral <italic>Acropora millepora</italic></article-title><alt-title alt-title-type="running">Coral larvae uptake diazotrophs</alt-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Lema</surname><given-names>Kimberley A</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref><xref ref-type="aff" rid="aff4">4</xref></contrib><contrib contrib-type="author"><name><surname>Clode</surname><given-names>Peta L</given-names></name><xref ref-type="aff" rid="aff5">5</xref><xref ref-type="aff" rid="aff6">6</xref></contrib><contrib contrib-type="author"><name><surname>Kilburn</surname><given-names>Matt R</given-names></name><xref ref-type="aff" rid="aff5">5</xref></contrib><contrib contrib-type="author"><name><surname>Thornton</surname><given-names>Ruth</given-names></name><xref ref-type="aff" rid="aff7">7</xref></contrib><contrib contrib-type="author"><name><surname>Willis</surname><given-names>Bette L</given-names></name><xref ref-type="aff" rid="aff1">1</xref><xref ref-type="aff" rid="aff3">3</xref></contrib><contrib contrib-type="author"><name><surname>Bourne</surname><given-names>David G</given-names></name><xref ref-type="aff" rid="aff2">2</xref><xref ref-type="aff" rid="aff3">3</xref><xref ref-type="corresp" rid="caf1">*</xref></contrib><aff id="aff1"><label>1</label><institution>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University</institution>, Townsville, Queensland,<country>Australia</country></aff><aff id="aff2"><label>2</label><institution>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC</institution>, Townsville, Queensland,<country>Australia</country></aff><aff id="aff3"><label>3</label><institution>AIMS@JCU, James Cook University</institution>, Townsville, Queensland,<country>Australia</country></aff><aff id="aff4"><label>4</label><institution>IFREMER, Dyneco Pelagos</institution>, Plouzané,<country>France</country></aff><aff id="aff5"><label>5</label><institution>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy</institution>, Crawley, Western Australia,<country>Australia</country></aff><aff id="aff6"><label>6</label><institution>The Oceans Institute, The University of Western Australia, Stirling Hwy</institution>, Crawley, Western Australia,<country>Australia</country></aff><aff id="aff7"><label>7</label><institution>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy</institution>, Crawley, Western Australia,<country>Australia</country></aff></contrib-group><author-notes><corresp id="caf1"><label>*</label><institution>Center for Marine Microbiology and Genetics, Australian Institute of Marine Science (AIMS)</institution>, PMB 3, Townsville MC, Queensland 4810, <country>Australia</country>. E-mail: <email>d.bourne@aims.gov.au</email></corresp></author-notes><pub-date pub-type="ppub"><month>07</month><year>2016</year></pub-date><pub-date pub-type="epub"><day>22</day><month>12</month><year>2015</year></pub-date><volume>10</volume><issue>7</issue><fpage>1804</fpage><lpage>1808</lpage><history><date date-type="received"><day>04</day><month>05</month><year>2015</year></date><date date-type="rev-recd"><day>26</day><month>10</month><year>2015</year></date><date date-type="accepted"><day>06</day><month>11</month><year>2015</year></date></history><permissions><copyright-statement>Copyright © 2015 International Society for Microbial Ecology</copyright-statement><copyright-year>2015</copyright-year><copyright-holder>International Society for Microbial Ecology</copyright-holder></permissions><abstract><p>Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N<sub>2</sub>) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence <italic>in situ</italic> hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of <sup>15</sup>N-enriched diazotrophic <italic>Vibrio</italic> sp. isolated from <italic>Acropora millepora</italic> into conspecific coral larvae. Incorporation of <italic>Vibrio</italic> sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where <italic>Vibrio</italic> cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.</p></abstract></article-meta></front></pmc></record>Pour manipuler ce document sous Unix (Dilib)
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