Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora.
Identifieur interne : 001C75 ( PubMed/Corpus ); précédent : 001C74; suivant : 001C76Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora.
Auteurs : Kimberley A. Lema ; Peta L. Clode ; Matt R. Kilburn ; Ruth Thornton ; Bette L. Willis ; David G. BourneSource :
- The ISME journal [ 1751-7370 ] ; 2016.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Nitrogen.
- metabolism : Nitrogen-Fixing Bacteria.
- microbiology : Anthozoa.
- Animals, In Situ Hybridization, Fluorescence, Larva, Nitrogen Fixation.
Abstract
Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N2) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of (15)N-enriched diazotrophic Vibrio sp. isolated from Acropora millepora into conspecific coral larvae. Incorporation of Vibrio sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where Vibrio cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.
DOI: 10.1038/ismej.2015.229
PubMed: 26696324
Links to Exploration step
pubmed:26696324Le document en format XML
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Kilburn</name><affiliation><nlm:affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Thornton, Ruth" sort="Thornton, Ruth" uniqKey="Thornton R" first="Ruth" last="Thornton">Ruth Thornton</name><affiliation><nlm:affiliation>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Willis, Bette L" sort="Willis, Bette L" uniqKey="Willis B" first="Bette L" last="Willis">Bette L. Willis</name><affiliation><nlm:affiliation>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Bourne, David G" sort="Bourne, David G" uniqKey="Bourne D" first="David G" last="Bourne">David G. 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Lema</name><affiliation><nlm:affiliation>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Clode, Peta L" sort="Clode, Peta L" uniqKey="Clode P" first="Peta L" last="Clode">Peta L. Clode</name><affiliation><nlm:affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Kilburn, Matt R" sort="Kilburn, Matt R" uniqKey="Kilburn M" first="Matt R" last="Kilburn">Matt R. Kilburn</name><affiliation><nlm:affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Thornton, Ruth" sort="Thornton, Ruth" uniqKey="Thornton R" first="Ruth" last="Thornton">Ruth Thornton</name><affiliation><nlm:affiliation>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Willis, Bette L" sort="Willis, Bette L" uniqKey="Willis B" first="Bette L" last="Willis">Bette L. Willis</name><affiliation><nlm:affiliation>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.</nlm:affiliation></affiliation></author><author><name sortKey="Bourne, David G" sort="Bourne, David G" uniqKey="Bourne D" first="David G" last="Bourne">David G. Bourne</name><affiliation><nlm:affiliation>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC, Townsville, Queensland, Australia.</nlm:affiliation></affiliation></author></analytic><series><title level="j">The ISME journal</title><idno type="eISSN">1751-7370</idno><imprint><date when="2016" type="published">2016</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Anthozoa (microbiology)</term><term>In Situ Hybridization, Fluorescence</term><term>Larva</term><term>Nitrogen (metabolism)</term><term>Nitrogen Fixation</term><term>Nitrogen-Fixing Bacteria (metabolism)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrogen</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nitrogen-Fixing Bacteria</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Anthozoa</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>In Situ Hybridization, Fluorescence</term><term>Larva</term><term>Nitrogen Fixation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N2) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of (15)N-enriched diazotrophic Vibrio sp. isolated from Acropora millepora into conspecific coral larvae. Incorporation of Vibrio sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where Vibrio cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26696324</PMID><DateCreated><Year>2016</Year><Month>06</Month><Day>21</Day></DateCreated><DateCompleted><Year>2017</Year><Month>09</Month><Day>26</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>13</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1751-7370</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>7</Issue><PubDate><Year>2016</Year><Month>07</Month></PubDate></JournalIssue><Title>The ISME journal</Title><ISOAbbreviation>ISME J</ISOAbbreviation></Journal><ArticleTitle>Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora.</ArticleTitle><Pagination><MedlinePgn>1804-8</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1038/ismej.2015.229</ELocationID><Abstract><AbstractText>Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N2) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of (15)N-enriched diazotrophic Vibrio sp. isolated from Acropora millepora into conspecific coral larvae. Incorporation of Vibrio sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where Vibrio cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lema</LastName><ForeName>Kimberley A</ForeName><Initials>KA</Initials><AffiliationInfo><Affiliation>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC, Townsville, Queensland, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>AIMS@JCU, James Cook University, Townsville, Queensland, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>IFREMER, Dyneco Pelagos, Plouzané, France.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Clode</LastName><ForeName>Peta L</ForeName><Initials>PL</Initials><AffiliationInfo><Affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>The Oceans Institute, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Kilburn</LastName><ForeName>Matt R</ForeName><Initials>MR</Initials><AffiliationInfo><Affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Thornton</LastName><ForeName>Ruth</ForeName><Initials>R</Initials><AffiliationInfo><Affiliation>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Willis</LastName><ForeName>Bette L</ForeName><Initials>BL</Initials><AffiliationInfo><Affiliation>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>AIMS@JCU, James Cook University, Townsville, Queensland, Australia.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bourne</LastName><ForeName>David G</ForeName><Initials>DG</Initials><AffiliationInfo><Affiliation>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC, Townsville, Queensland, Australia.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>AIMS@JCU, James Cook University, Townsville, Queensland, Australia.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2015</Year><Month>12</Month><Day>22</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>ISME J</MedlineTA><NlmUniqueID>101301086</NlmUniqueID><ISSNLinking>1751-7362</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>N762921K75</RegistryNumber><NameOfSubstance UI="D009584">Nitrogen</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="Cites"><RefSource>Mol Ecol. 2014 Oct;23(19):4682-95</RefSource><PMID Version="1">25156176</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>ISME J. 2014 Nov;8(11):2272-9</RefSource><PMID Version="1">24830827</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Science. 2004 Aug 13;305(5686):997-1000</RefSource><PMID Version="1">15310901</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>ISME J. 2012 Jul;6(7):1314-24</RefSource><PMID Version="1">22222466</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Plant Cell Environ. 2008 May;31(5):679-94</RefSource><PMID Version="1">18315536</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Appl Environ Microbiol. 2012 May;78(9):3136-44</RefSource><PMID Version="1">22344646</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Environ Microbiol. 2015 Oct;17(10):3570-80</RefSource><PMID Version="1">24902979</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Environ Microbiol. 2014 Oct;16(10):3345-59</RefSource><PMID Version="1">24373029</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>Environ Microbiol. 2010 Feb;12(2):541-56</RefSource><PMID Version="1">19958382</PMID></CommentsCorrections><CommentsCorrections RefType="Cites"><RefSource>MBio. 2013 May 14;4(3):e00052-13</RefSource><PMID Version="1">23674611</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D037421" MajorTopicYN="N">Anthozoa</DescriptorName><QualifierName UI="Q000382" MajorTopicYN="Y">microbiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017404" MajorTopicYN="N">In Situ Hybridization, Fluorescence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007814" MajorTopicYN="N">Larva</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D009584" MajorTopicYN="N">Nitrogen</DescriptorName><QualifierName UI="Q000378" 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