Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora
Identifieur interne : 002F07 ( Ncbi/Merge ); précédent : 002F06; suivant : 002F08Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora
Auteurs : Kimberley A. Lema [Australie, France] ; Peta L. Clode [Australie] ; Matt R. Kilburn [Australie] ; Ruth Thornton [Australie] ; Bette L. Willis [Australie] ; David G. Bourne [Australie]Source :
- The ISME Journal [ 1751-7362 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
- microbiologie : Anthozoa.
- métabolisme : Azote, Bactéries fixatrices d'azote.
- Animaux, Fixation de l'azote, Larve, Technique FISH.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Nitrogen.
- metabolism : Nitrogen-Fixing Bacteria.
- microbiology : Anthozoa.
- Animals, In Situ Hybridization, Fluorescence, Larva, Nitrogen Fixation.
Abstract
Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N2) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence
Url:
DOI: 10.1038/ismej.2015.229
PubMed: 26696324
PubMed Central: 4918436
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PMC:4918436Le document en format XML
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<series><title level="j">The ISME Journal</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Anthozoa (microbiology)</term>
<term>In Situ Hybridization, Fluorescence</term>
<term>Larva</term>
<term>Nitrogen (metabolism)</term>
<term>Nitrogen Fixation</term>
<term>Nitrogen-Fixing Bacteria (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anthozoa (microbiologie)</term>
<term>Azote (métabolisme)</term>
<term>Bactéries fixatrices d'azote (métabolisme)</term>
<term>Fixation de l'azote</term>
<term>Larve</term>
<term>Technique FISH</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrogen</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nitrogen-Fixing Bacteria</term>
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<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Anthozoa</term>
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<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Anthozoa</term>
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<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term>
<term>Bactéries fixatrices d'azote</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>In Situ Hybridization, Fluorescence</term>
<term>Larva</term>
<term>Nitrogen Fixation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Fixation de l'azote</term>
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<front><div type="abstract" xml:lang="en"><p>Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N<sub>2</sub>
) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence <italic>in situ</italic>
hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of <sup>15</sup>
N-enriched diazotrophic <italic>Vibrio</italic>
sp. isolated from <italic>Acropora millepora</italic>
into conspecific coral larvae. Incorporation of <italic>Vibrio</italic>
sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where <italic>Vibrio</italic>
cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.</p>
</div>
</front>
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</affiliation>
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, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff4"><institution>IFREMER, Dyneco Pelagos</institution>
, Plouzané,<country>France</country>
</nlm:aff>
<country xml:lang="fr">France</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Clode, Peta L" sort="Clode, Peta L" uniqKey="Clode P" first="Peta L" last="Clode">Peta L. Clode</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff6"><institution>The Oceans Institute, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Kilburn, Matt R" sort="Kilburn, Matt R" uniqKey="Kilburn M" first="Matt R" last="Kilburn">Matt R. Kilburn</name>
<affiliation wicri:level="1"><nlm:aff id="aff5"><institution>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Thornton, Ruth" sort="Thornton, Ruth" uniqKey="Thornton R" first="Ruth" last="Thornton">Ruth Thornton</name>
<affiliation wicri:level="1"><nlm:aff id="aff7"><institution>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy</institution>
, Crawley, Western Australia,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Willis, Bette L" sort="Willis, Bette L" uniqKey="Willis B" first="Bette L" last="Willis">Bette L. Willis</name>
<affiliation wicri:level="1"><nlm:aff id="aff1"><institution>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
<author><name sortKey="Bourne, David G" sort="Bourne, David G" uniqKey="Bourne D" first="David G" last="Bourne">David G. Bourne</name>
<affiliation wicri:level="1"><nlm:aff id="aff2"><institution>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff3"><institution>AIMS@JCU, James Cook University</institution>
, Townsville, Queensland,<country>Australia</country>
</nlm:aff>
<country xml:lang="fr">Australie</country>
<wicri:regionArea># see nlm:aff country strict</wicri:regionArea>
</affiliation>
</author>
</analytic>
<series><title level="j">The ISME Journal</title>
<idno type="ISSN">1751-7362</idno>
<idno type="eISSN">1751-7370</idno>
<imprint><date when="2015">2015</date>
</imprint>
</series>
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</profileDesc>
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<front><div type="abstract" xml:lang="en"><p>Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N<sub>2</sub>
) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence <italic>in situ</italic>
hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of <sup>15</sup>
N-enriched diazotrophic <italic>Vibrio</italic>
sp. isolated from <italic>Acropora millepora</italic>
into conspecific coral larvae. Incorporation of <italic>Vibrio</italic>
sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where <italic>Vibrio</italic>
cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.</p>
</div>
</front>
</TEI>
</pmc>
<pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora.</title>
<author><name sortKey="Lema, Kimberley A" sort="Lema, Kimberley A" uniqKey="Lema K" first="Kimberley A" last="Lema">Kimberley A. Lema</name>
<affiliation wicri:level="1"><nlm:affiliation>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland</wicri:regionArea>
<wicri:noRegion>Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Clode, Peta L" sort="Clode, Peta L" uniqKey="Clode P" first="Peta L" last="Clode">Peta L. Clode</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia</wicri:regionArea>
<wicri:noRegion>Western Australia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kilburn, Matt R" sort="Kilburn, Matt R" uniqKey="Kilburn M" first="Matt R" last="Kilburn">Matt R. Kilburn</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia</wicri:regionArea>
<wicri:noRegion>Western Australia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Thornton, Ruth" sort="Thornton, Ruth" uniqKey="Thornton R" first="Ruth" last="Thornton">Ruth Thornton</name>
<affiliation wicri:level="1"><nlm:affiliation>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>School of Paediatrics and Child Heath, The University of Western Australia, Stirling Hwy, Crawley, Western Australia</wicri:regionArea>
<wicri:noRegion>Western Australia</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Willis, Bette L" sort="Willis, Bette L" uniqKey="Willis B" first="Bette L" last="Willis">Bette L. Willis</name>
<affiliation wicri:level="1"><nlm:affiliation>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland</wicri:regionArea>
<wicri:noRegion>Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bourne, David G" sort="Bourne, David G" uniqKey="Bourne D" first="David G" last="Bourne">David G. Bourne</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC, Townsville, Queensland, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC, Townsville, Queensland</wicri:regionArea>
<wicri:noRegion>Queensland</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
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<date when="2016">2016</date>
<idno type="RBID">pubmed:26696324</idno>
<idno type="pmid">26696324</idno>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Imaging the uptake of nitrogen-fixing bacteria into larvae of the coral Acropora millepora.</title>
<author><name sortKey="Lema, Kimberley A" sort="Lema, Kimberley A" uniqKey="Lema K" first="Kimberley A" last="Lema">Kimberley A. Lema</name>
<affiliation wicri:level="1"><nlm:affiliation>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland</wicri:regionArea>
<wicri:noRegion>Queensland</wicri:noRegion>
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</author>
<author><name sortKey="Clode, Peta L" sort="Clode, Peta L" uniqKey="Clode P" first="Peta L" last="Clode">Peta L. Clode</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia</wicri:regionArea>
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</affiliation>
</author>
<author><name sortKey="Kilburn, Matt R" sort="Kilburn, Matt R" uniqKey="Kilburn M" first="Matt R" last="Kilburn">Matt R. Kilburn</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Microscopy, Characterisation and Analysis, The University of Western Australia, Stirling Hwy, Crawley, Western Australia, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
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<author><name sortKey="Thornton, Ruth" sort="Thornton, Ruth" uniqKey="Thornton R" first="Ruth" last="Thornton">Ruth Thornton</name>
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<author><name sortKey="Willis, Bette L" sort="Willis, Bette L" uniqKey="Willis B" first="Bette L" last="Willis">Bette L. Willis</name>
<affiliation wicri:level="1"><nlm:affiliation>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>ARC Centre of Excellence for Coral Reef Studies, and College of Marine and Environmental Sciences, James Cook University, Townsville, Queensland</wicri:regionArea>
<wicri:noRegion>Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bourne, David G" sort="Bourne, David G" uniqKey="Bourne D" first="David G" last="Bourne">David G. Bourne</name>
<affiliation wicri:level="1"><nlm:affiliation>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC, Townsville, Queensland, Australia.</nlm:affiliation>
<country xml:lang="fr">Australie</country>
<wicri:regionArea>Centre for Marine Microbiology and Genetics, Australian Institute of Marine Science, Townsville MC, Townsville, Queensland</wicri:regionArea>
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<series><title level="j">The ISME journal</title>
<idno type="eISSN">1751-7370</idno>
<imprint><date when="2016" type="published">2016</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Anthozoa (microbiology)</term>
<term>In Situ Hybridization, Fluorescence</term>
<term>Larva</term>
<term>Nitrogen (metabolism)</term>
<term>Nitrogen Fixation</term>
<term>Nitrogen-Fixing Bacteria (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anthozoa (microbiologie)</term>
<term>Azote (métabolisme)</term>
<term>Bactéries fixatrices d'azote (métabolisme)</term>
<term>Fixation de l'azote</term>
<term>Larve</term>
<term>Technique FISH</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nitrogen</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Nitrogen-Fixing Bacteria</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Anthozoa</term>
</keywords>
<keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Anthozoa</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Azote</term>
<term>Bactéries fixatrices d'azote</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>In Situ Hybridization, Fluorescence</term>
<term>Larva</term>
<term>Nitrogen Fixation</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Fixation de l'azote</term>
<term>Larve</term>
<term>Technique FISH</term>
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<front><div type="abstract" xml:lang="en">Diazotrophic bacteria are instrumental in generating biologically usable forms of nitrogen by converting abundant dinitrogen gas (N2) into available forms, such as ammonium. Although nitrogen is crucial for coral growth, direct observation of associations between diazotrophs and corals has previously been elusive. We applied fluorescence in situ hybridization (FISH) and nanoscale secondary ion mass spectrometry to observe the uptake of (15)N-enriched diazotrophic Vibrio sp. isolated from Acropora millepora into conspecific coral larvae. Incorporation of Vibrio sp. cells was observed in coral larvae after 4-h incubation with enriched bacteria. Uptake was restricted to the aboral epidermis of larvae, where Vibrio cells clustered in elongated aggregations. Other bacterial associates were also observed in epidermal areas in FISH analyses. Although the fate and role of these bacteria requires additional investigation, this study describes a powerful approach to further explore cell associations and nutritional pathways in the early life stages of the coral holobiont.</div>
</front>
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