Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation
Identifieur interne : 001B81 ( Main/Merge ); précédent : 001B80; suivant : 001B82Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation
Auteurs : Zehava Faltin [Israël] ; Doron Holland [Israël] ; Margarita Velcheva [Canada] ; Marina Tsapovetsky [Israël] ; Patricia Roeckel-Drevet [France] ; Avtar K. Handa [États-Unis] ; Mohamad Abu-Abied [Israël] ; Miriam Friedman-Einat [Israël] ; Yuval Eshdat [Israël] ; Avihai Perl [Israël]Source :
- Plant and Cell Physiology [ 0032-0781 ] ; 2010-07.
English descriptors
- KwdEn :
- Citrus (enzymology), Gene Expression Regulation, Plant, Glutathione Peroxidase (metabolism), Homeostasis, Mutagenesis, Site-Directed, Oxidation-Reduction, Oxidative Stress, Plant Shoots (growth & development), Plants, Genetically Modified (growth & development), Plants, Genetically Modified (metabolism), Reactive Oxygen Species (metabolism), Regeneration, Stress, Physiological, Tobacco (growth & development), Tobacco (metabolism), Transformation, Genetic.
- MESH :
- chemical , metabolism : Glutathione Peroxidase, Reactive Oxygen Species.
- enzymology : Citrus.
- growth & development : Plant Shoots, Plants, Genetically Modified, Tobacco.
- metabolism : Plants, Genetically Modified, Tobacco.
- mix :
Abstract
Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.
Url:
- https://api.istex.fr/document/A4D64ABF200EC222D26122C38E3F624D8E26005C/fulltext/pdf
- https://hal.archives-ouvertes.fr/hal-00964726
DOI: 10.1093/pcp/pcq082
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type="ISSN">0032-0781</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus (enzymology)</term><term>Gene Expression Regulation, Plant</term><term>Glutathione Peroxidase (metabolism)</term><term>Homeostasis</term><term>Mutagenesis, Site-Directed</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term><term>Plant Shoots (growth & development)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term><term>Regeneration</term><term>Stress, Physiological</term><term>Tobacco (growth & development)</term><term>Tobacco (metabolism)</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione Peroxidase</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Shoots</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="mix" xml:lang="en"><term>GLUTATHIONE PEROXIDASE</term><term>Gene Expression Regulation, Plant</term><term>Homeostasis</term><term>Mutagenesis, Site-Directed</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term><term>REACTIVE OXYGEN SPECIES</term><term>REDOC REGULATION</term><term>Regeneration</term><term>SHOOT REGENERATION</term><term>Stress, Physiological</term><term>TRANSGENIC PLANTS</term><term>Transformation, Genetic</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</div></front></TEI><double doi="10.1093/pcp/pcq082"><HAL><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation</title><author><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" 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status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Israël</country></affiliation></author><author><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation 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active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Israël</country></affiliation></author><author><name sortKey="Roeckel Drevet, Patricia" sort="Roeckel Drevet, Patricia" uniqKey="Roeckel Drevet P" first="Patricia" last="Roeckel-Drevet">Patricia Roeckel-Drevet</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-26029" status="VALID"><orgName>Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier</orgName><orgName type="acronym">PIAF</orgName><desc><address><addrLine>INRA Site de Crouël 234, avenue du Brézet 63100 Clermont-Ferrand - France</addrLine><country key="FR"></country></address><ref type="url">http://www6.clermont.inra.fr/piaf</ref></desc><listRelation><relation name="UMRA547" active="#struct-92114" type="direct"></relation><relation active="#struct-205618" type="direct"></relation></listRelation><tutelles><tutelle name="UMRA547" active="#struct-92114" type="direct"><org type="institution" xml:id="struct-92114" status="VALID"><orgName>Institut National de la Recherche Agronomique</orgName><orgName type="acronym">INRA</orgName><desc><address><country key="FR"></country></address><ref type="url">http://www.inra.fr</ref></desc></org></tutelle><tutelle active="#struct-205618" type="direct"><org type="institution" xml:id="struct-205618" status="VALID"><orgName>Université Blaise Pascal - Clermont-Ferrand 2</orgName><orgName type="acronym">UBP</orgName><desc><address><addrLine>34, avenue Carnot - BP 185 - 63006 Clermont-Ferrand cedex</addrLine><country key="FR"></country></address><ref type="url">http://www.univ-bpclermont.fr/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>France</country></affiliation></author><author><name sortKey="Handa, Avtar" sort="Handa, Avtar" uniqKey="Handa A" first="Avtar" last="Handa">Avtar Handa</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-249048" status="INCOMING"><orgName>Department of Horticulture and Landscape Architecture</orgName><desc><address><addrLine>West Lafayette</addrLine><country key="US"></country></address></desc><listRelation><relation active="#struct-147250" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-147250" type="direct"><org type="institution" xml:id="struct-147250" status="VALID"><orgName>Purdue University [West Lafayette]</orgName><desc><address><addrLine>Hovde Hall, 610 Purdue Mall, West Lafayette, IN 47907</addrLine><country key="US"></country></address><ref type="url">http://www.purdue.edu/</ref></desc></org></tutelle></tutelles></hal:affiliation><country>États-Unis</country></affiliation></author><author><name sortKey="Abu Abied, Mohamad" sort="Abu Abied, Mohamad" uniqKey="Abu Abied M" first="Mohamad" last="Abu-Abied">Mohamad Abu-Abied</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Israël</country></affiliation></author><author><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Israël</country></affiliation></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Israël</country></affiliation></author><author><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name><affiliation wicri:level="1"><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation><country>Israël</country></affiliation></author></analytic><idno type="DOI">10.1093/pcp/pcq082</idno><series><title level="j">Plant and Cell Physiology</title><idno type="ISSN">0032-0781</idno><imprint><date type="datePub">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>GLUTATHIONE PEROXIDASE</term><term>REACTIVE OXYGEN SPECIES</term><term>REDOC REGULATION</term><term>SHOOT REGENERATION</term><term>TRANSGENIC PLANTS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme's catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a beta-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</div></front></TEI></HAL><ISTEX><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation</title><author><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name></author><author><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name></author><author><name sortKey="Velcheva, Margarita" sort="Velcheva, Margarita" uniqKey="Velcheva M" first="Margarita" last="Velcheva">Margarita Velcheva</name></author><author><name sortKey="Tsapovetsky, Marina" sort="Tsapovetsky, Marina" uniqKey="Tsapovetsky M" first="Marina" last="Tsapovetsky">Marina Tsapovetsky</name></author><author><name sortKey="Roeckel Drevet, Patricia" sort="Roeckel Drevet, Patricia" uniqKey="Roeckel Drevet P" first="Patricia" last="Roeckel-Drevet">Patricia Roeckel-Drevet</name></author><author><name sortKey="Handa, Avtar K" sort="Handa, Avtar K" uniqKey="Handa A" first="Avtar K." last="Handa">Avtar K. Handa</name></author><author><name sortKey="Abu Abied, Mohamad" sort="Abu Abied, Mohamad" uniqKey="Abu Abied M" first="Mohamad" last="Abu-Abied">Mohamad Abu-Abied</name></author><author><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name></author><author><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:A4D64ABF200EC222D26122C38E3F624D8E26005C</idno><date when="2010" year="2010">2010</date><idno type="doi">10.1093/pcp/pcq082</idno><idno type="url">https://api.istex.fr/document/A4D64ABF200EC222D26122C38E3F624D8E26005C/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000A87</idno><idno type="wicri:Area/Istex/Curation">000A87</idno><idno type="wicri:Area/Istex/Checkpoint">000350</idno><idno type="wicri:doubleKey">0032-0781:2010:Faltin Z:glutathione:peroxidase:regulation</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation</title><author><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Velcheva, Margarita" sort="Velcheva, Margarita" uniqKey="Velcheva M" first="Margarita" last="Velcheva">Margarita Velcheva</name><affiliation wicri:level="1"><country xml:lang="fr">Canada</country><wicri:regionArea>Alberta Research Council, PO Bag 4000, Hwy 16A and 75 Street, Vegreville, AB, T9C 1T4</wicri:regionArea><wicri:noRegion>T9C 1T4</wicri:noRegion></affiliation></author><author><name sortKey="Tsapovetsky, Marina" sort="Tsapovetsky, Marina" uniqKey="Tsapovetsky M" first="Marina" last="Tsapovetsky">Marina Tsapovetsky</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Roeckel Drevet, Patricia" sort="Roeckel Drevet, Patricia" uniqKey="Roeckel Drevet P" first="Patricia" last="Roeckel-Drevet">Patricia Roeckel-Drevet</name><affiliation wicri:level="4"><country xml:lang="fr">France</country><wicri:regionArea>UMR INRA 547-PIAF, Université Blaise Pascal, 63177 Aubière Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Auvergne-Rhône-Alpes</region><region type="old region" nuts="2">Auvergne (région administrative)</region><settlement type="city">Aubière</settlement></placeName><orgName type="university">Université Blaise-Pascal</orgName></affiliation></author><author><name sortKey="Handa, Avtar K" sort="Handa, Avtar K" uniqKey="Handa A" first="Avtar K." last="Handa">Avtar K. Handa</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Indiana</region></placeName><wicri:cityArea>Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Dr., West Lafayette</wicri:cityArea></affiliation></author><author><name sortKey="Abu Abied, Mohamad" sort="Abu Abied, Mohamad" uniqKey="Abu Abied M" first="Mohamad" last="Abu-Abied">Mohamad Abu-Abied</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Animal Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Present address: The Chief Scientist of Israel Ministry of Agriculture & Rural Development, PO Box 30, 50250 Bet-Dagan</wicri:regionArea><wicri:noRegion>50250 Bet-Dagan</wicri:noRegion></affiliation><affiliation wicri:level="1"><country wicri:rule="url">Israël</country></affiliation></author><author><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name><affiliation wicri:level="1"><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant and Cell Physiology</title><idno type="ISSN">0032-0781</idno><idno type="eISSN">1471-9053</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2010-07">2010-07</date><biblScope unit="volume">51</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1151">1151</biblScope><biblScope unit="page" to="1162">1162</biblScope></imprint><idno type="ISSN">0032-0781</idno></series><idno type="istex">A4D64ABF200EC222D26122C38E3F624D8E26005C</idno><idno type="DOI">10.1093/pcp/pcq082</idno><idno type="ArticleID">pcq082</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0032-0781</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</div></front></TEI></ISTEX><PubMed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation.</title><author><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel.</nlm:affiliation><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name></author><author><name sortKey="Velcheva, Margarita" sort="Velcheva, Margarita" uniqKey="Velcheva M" first="Margarita" last="Velcheva">Margarita Velcheva</name></author><author><name sortKey="Tsapovetsky, Marina" sort="Tsapovetsky, Marina" uniqKey="Tsapovetsky M" first="Marina" last="Tsapovetsky">Marina Tsapovetsky</name></author><author><name sortKey="Roeckel Drevet, Patricia" sort="Roeckel Drevet, Patricia" uniqKey="Roeckel Drevet P" first="Patricia" last="Roeckel-Drevet">Patricia Roeckel-Drevet</name></author><author><name sortKey="Handa, Avtar K" sort="Handa, Avtar K" uniqKey="Handa A" first="Avtar K" last="Handa">Avtar K. Handa</name></author><author><name sortKey="Abu Abied, Mohamad" sort="Abu Abied, Mohamad" uniqKey="Abu Abied M" first="Mohamad" last="Abu-Abied">Mohamad Abu-Abied</name></author><author><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name></author><author><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20530511</idno><idno type="pmid">20530511</idno><idno type="doi">10.1093/pcp/pcq082</idno><idno type="wicri:Area/PubMed/Corpus">000823</idno><idno type="wicri:Area/PubMed/Curation">000823</idno><idno type="wicri:Area/PubMed/Checkpoint">000823</idno><idno type="wicri:Area/Ncbi/Merge">000B85</idno><idno type="wicri:Area/Ncbi/Curation">000B85</idno><idno type="wicri:Area/Ncbi/Checkpoint">000B85</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation.</title><author><name sortKey="Faltin, Zehava" sort="Faltin, Zehava" uniqKey="Faltin Z" first="Zehava" last="Faltin">Zehava Faltin</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel.</nlm:affiliation><country xml:lang="fr">Israël</country><wicri:regionArea>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan</wicri:regionArea><wicri:noRegion>50250 Bet Dagan</wicri:noRegion></affiliation></author><author><name sortKey="Holland, Doron" sort="Holland, Doron" uniqKey="Holland D" first="Doron" last="Holland">Doron Holland</name></author><author><name sortKey="Velcheva, Margarita" sort="Velcheva, Margarita" uniqKey="Velcheva M" first="Margarita" last="Velcheva">Margarita Velcheva</name></author><author><name sortKey="Tsapovetsky, Marina" sort="Tsapovetsky, Marina" uniqKey="Tsapovetsky M" first="Marina" last="Tsapovetsky">Marina Tsapovetsky</name></author><author><name sortKey="Roeckel Drevet, Patricia" sort="Roeckel Drevet, Patricia" uniqKey="Roeckel Drevet P" first="Patricia" last="Roeckel-Drevet">Patricia Roeckel-Drevet</name></author><author><name sortKey="Handa, Avtar K" sort="Handa, Avtar K" uniqKey="Handa A" first="Avtar K" last="Handa">Avtar K. Handa</name></author><author><name sortKey="Abu Abied, Mohamad" sort="Abu Abied, Mohamad" uniqKey="Abu Abied M" first="Mohamad" last="Abu-Abied">Mohamad Abu-Abied</name></author><author><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name></author><author><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="eISSN">1471-9053</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Citrus (enzymology)</term><term>Gene Expression Regulation, Plant</term><term>Glutathione Peroxidase (metabolism)</term><term>Homeostasis</term><term>Mutagenesis, Site-Directed</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term><term>Plant Shoots (growth & development)</term><term>Plants, Genetically Modified (growth & development)</term><term>Plants, Genetically Modified (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term><term>Regeneration</term><term>Stress, Physiological</term><term>Tobacco (growth & development)</term><term>Tobacco (metabolism)</term><term>Transformation, Genetic</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glutathione Peroxidase</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Shoots</term><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Plants, Genetically Modified</term><term>Tobacco</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Homeostasis</term><term>Mutagenesis, Site-Directed</term><term>Oxidation-Reduction</term><term>Oxidative Stress</term><term>Regeneration</term><term>Stress, Physiological</term><term>Transformation, Genetic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme's catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a beta-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</div></front></TEI></PubMed></double></record>Pour manipuler ce document sous Unix (Dilib)
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