Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation
Identifieur interne : 000A87 ( Istex/Corpus ); précédent : 000A86; suivant : 000A88Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation
Auteurs : Zehava Faltin ; Doron Holland ; Margarita Velcheva ; Marina Tsapovetsky ; Patricia Roeckel-Drevet ; Avtar K. Handa ; Mohamad Abu-Abied ; Miriam Friedman-Einat ; Yuval Eshdat ; Avihai PerlSource :
- Plant and Cell Physiology [ 0032-0781 ] ; 2010-07.
Abstract
Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.
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DOI: 10.1093/pcp/pcq082
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Handa</name><affiliation><mods:affiliation>Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Dr., West Lafayette, IN 47907-2010 USA</mods:affiliation></affiliation></author><author><name sortKey="Abu Abied, Mohamad" sort="Abu Abied, Mohamad" uniqKey="Abu Abied M" first="Mohamad" last="Abu-Abied">Mohamad Abu-Abied</name><affiliation><mods:affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</mods:affiliation></affiliation></author><author><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name><affiliation><mods:affiliation>Institute of Animal Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</mods:affiliation></affiliation></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><affiliation><mods:affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</mods:affiliation></affiliation><affiliation><mods:affiliation>Present address: The Chief Scientist of Israel Ministry of Agriculture & Rural Development, PO Box 30, 50250 Bet-Dagan, Israel</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: vhyuval@agri.gov.il</mods:affiliation></affiliation></author><author><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name><affiliation><mods:affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Plant and Cell Physiology</title><idno type="ISSN">0032-0781</idno><idno type="eISSN">1471-9053</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2010-07">2010-07</date><biblScope unit="volume">51</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1151">1151</biblScope><biblScope unit="page" to="1162">1162</biblScope></imprint><idno type="ISSN">0032-0781</idno></series><idno type="istex">A4D64ABF200EC222D26122C38E3F624D8E26005C</idno><idno type="DOI">10.1093/pcp/pcq082</idno><idno type="ArticleID">pcq082</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0032-0781</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract">Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</div></front></TEI><istex><corpusName>oup</corpusName><author><json:item><name>Zehava Faltin</name><affiliations><json:string>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</json:string></affiliations></json:item><json:item><name>Doron Holland</name><affiliations><json:string>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</json:string></affiliations></json:item><json:item><name>Margarita Velcheva</name><affiliations><json:string>Alberta Research Council, PO Bag 4000, Hwy 16A and 75 Street, Vegreville, AB, T9C 1T4, Canada</json:string></affiliations></json:item><json:item><name>Marina Tsapovetsky</name><affiliations><json:string>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</json:string></affiliations></json:item><json:item><name>Patricia Roeckel-Drevet</name><affiliations><json:string>UMR INRA 547-PIAF, Université Blaise Pascal, 63177 Aubière Cedex, France</json:string></affiliations></json:item><json:item><name>Avtar K. 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To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. 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Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org</p></availability><date>2010-06-08</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation</title><author xml:id="author-1"><persName><forename type="first">Zehava</forename><surname>Faltin</surname></persName><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation></author><author xml:id="author-2"><persName><forename type="first">Doron</forename><surname>Holland</surname></persName><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation></author><author xml:id="author-3"><persName><forename type="first">Margarita</forename><surname>Velcheva</surname></persName><affiliation>Alberta Research Council, PO Bag 4000, Hwy 16A and 75 Street, Vegreville, AB, T9C 1T4, Canada</affiliation></author><author xml:id="author-4"><persName><forename type="first">Marina</forename><surname>Tsapovetsky</surname></persName><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation></author><author xml:id="author-5"><persName><forename type="first">Patricia</forename><surname>Roeckel-Drevet</surname></persName><affiliation>UMR INRA 547-PIAF, Université Blaise Pascal, 63177 Aubière Cedex, France</affiliation></author><author xml:id="author-6"><persName><forename type="first">Avtar K.</forename><surname>Handa</surname></persName><affiliation>Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Dr., West Lafayette, IN 47907-2010 USA</affiliation></author><author xml:id="author-7"><persName><forename type="first">Mohamad</forename><surname>Abu-Abied</surname></persName><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation></author><author xml:id="author-8"><persName><forename type="first">Miriam</forename><surname>Friedman-Einat</surname></persName><affiliation>Institute of Animal Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation></author><author xml:id="author-9" corresp="yes"><persName><forename type="first">Yuval</forename><surname>Eshdat</surname></persName><email>vhyuval@agri.gov.il</email><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation><affiliation>Present address: The Chief Scientist of Israel Ministry of Agriculture & Rural Development, PO Box 30, 50250 Bet-Dagan, Israel</affiliation></author><author xml:id="author-10"><persName><forename type="first">Avihai</forename><surname>Perl</surname></persName><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation></author></analytic><monogr><title level="j">Plant and Cell Physiology</title><idno type="pISSN">0032-0781</idno><idno type="eISSN">1471-9053</idno><imprint><publisher>Oxford University Press</publisher><date type="published" when="2010-07"></date><biblScope unit="volume">51</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="page" from="1151">1151</biblScope><biblScope unit="page" to="1162">1162</biblScope></imprint></monogr><idno type="istex">A4D64ABF200EC222D26122C38E3F624D8E26005C</idno><idno type="DOI">10.1093/pcp/pcq082</idno><idno type="ArticleID">pcq082</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2010-06-08</date></creation><langUsage><language ident="en">en</language></langUsage><abstract><p>Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</p></abstract><textClass><keywords scheme="keyword"><list><item><term>Regular Papers</term></item></list></keywords></textClass><textClass><keywords scheme="keyword"><list><head>keywords</head><item><term>Glutathione peroxidase</term></item><item><term>Nicotiana tabacum</term></item><item><term>Reactive oxygen species</term></item><item><term>Redox regulation</term></item><item><term>Shoot regeneration</term></item><item><term>Transgenic plants</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2010-06-08">Created</change><change when="2010-07">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><original>false</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/A4D64ABF200EC222D26122C38E3F624D8E26005C/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus oup" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v2.3 20070202//EN" URI="journalpublishing.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article"><front><journal-meta><journal-id journal-id-type="hwp">pcellphys</journal-id><journal-id journal-id-type="publisher-id">pcp</journal-id><journal-title>Plant and Cell Physiology</journal-title><issn pub-type="ppub">0032-0781</issn><issn pub-type="epub">1471-9053</issn><publisher><publisher-name>Oxford University Press</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.1093/pcp/pcq082</article-id><article-id pub-id-type="publisher-id">pcq082</article-id><article-categories><subj-group><subject>Regular Papers</subject></subj-group><series-title>editor's choice</series-title></article-categories><title-group><article-title>Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Faltin</surname><given-names>Zehava</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Holland</surname><given-names>Doron</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Velcheva</surname><given-names>Margarita</given-names></name><xref ref-type="aff" rid="AFF2"><sup>2</sup></xref></contrib><contrib contrib-type="author"><name><surname>Tsapovetsky</surname><given-names>Marina</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Roeckel-Drevet</surname><given-names>Patricia</given-names></name><xref ref-type="aff" rid="AFF3"><sup>3</sup></xref></contrib><contrib contrib-type="author"><name><surname>Handa</surname><given-names>Avtar K.</given-names></name><xref ref-type="aff" rid="AFF4"><sup>4</sup></xref></contrib><contrib contrib-type="author"><name><surname>Abu-Abied</surname><given-names>Mohamad</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Friedman-Einat</surname><given-names>Miriam</given-names></name><xref ref-type="aff" rid="AFF5"><sup>5</sup></xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Eshdat</surname><given-names>Yuval</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref><xref ref-type="aff" rid="AFF6"><sup>6</sup></xref><xref ref-type="corresp" rid="COR1">* </xref></contrib><contrib contrib-type="author"><name><surname>Perl</surname><given-names>Avihai</given-names></name><xref ref-type="aff" rid="AFF1"><sup>1</sup></xref></contrib></contrib-group><aff id="AFF1"><sup>1</sup>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</aff><aff id="AFF2"><sup>2</sup>Alberta Research Council, PO Bag 4000, Hwy 16A and 75 Street, Vegreville, AB, T9C 1T4, Canada</aff><aff id="AFF3"><sup>3</sup>UMR INRA 547-PIAF, Université Blaise Pascal, 63177 Aubière Cedex, France</aff><aff id="AFF4"><sup>4</sup>Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Dr., West Lafayette, IN 47907-2010 USA</aff><aff id="AFF5"><sup>5</sup>Institute of Animal Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</aff><aff id="AFF6"><sup>6</sup>Present address: The Chief Scientist of Israel Ministry of Agriculture & Rural Development, PO Box 30, 50250 Bet-Dagan, Israel</aff><author-notes><corresp id="COR1">*Corresponding author: E-mail, <email>vhyuval@agri.gov.il</email>; Fax: <fax>+972-3-9697193</fax></corresp></author-notes><pub-date pub-type="ppub"><month>7</month><year>2010</year></pub-date><pub-date pub-type="epub"><day>8</day><month>6</month><year>2010</year></pub-date><volume>51</volume><issue>7</issue><fpage>1151</fpage><lpage>1162</lpage><history><date date-type="received"><day>31</day><month>3</month><year>2010</year></date><date date-type="accepted"><day>2</day><month>6</month><year>2010</year></date></history><permissions><copyright-statement>© The Author 2010. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For permissions, please email: journals.permissions@oxfordjournals.org</copyright-statement><copyright-year>2010</copyright-year></permissions><abstract><p>Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (<italic>Citrus sinensis</italic>) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (<italic>Nicotiana tabacum</italic>) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</p></abstract><kwd-group><kwd>Glutathione peroxidase</kwd><kwd><italic>Nicotiana tabacum</italic></kwd><kwd>Reactive oxygen species</kwd><kwd>Redox regulation</kwd><kwd>Shoot regeneration</kwd><kwd>Transgenic plants</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><partName>editor's choice</partName><title>Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><partName>editor's choice</partName><title>Glutathione Peroxidase Regulation of Reactive Oxygen Species Level is Crucial for In Vitro Plant Differentiation</title></titleInfo><name type="personal"><namePart type="given">Zehava</namePart><namePart type="family">Faltin</namePart><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Doron</namePart><namePart type="family">Holland</namePart><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Margarita</namePart><namePart type="family">Velcheva</namePart><affiliation>Alberta Research Council, PO Bag 4000, Hwy 16A and 75 Street, Vegreville, AB, T9C 1T4, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Marina</namePart><namePart type="family">Tsapovetsky</namePart><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Patricia</namePart><namePart type="family">Roeckel-Drevet</namePart><affiliation>UMR INRA 547-PIAF, Université Blaise Pascal, 63177 Aubière Cedex, France</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Avtar K.</namePart><namePart type="family">Handa</namePart><affiliation>Department of Horticulture and Landscape Architecture, Purdue University, 625 Agriculture Mall Dr., West Lafayette, IN 47907-2010 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Mohamad</namePart><namePart type="family">Abu-Abied</namePart><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Miriam</namePart><namePart type="family">Friedman-Einat</namePart><affiliation>Institute of Animal Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Yuval</namePart><namePart type="family">Eshdat</namePart><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation><affiliation>Present address: The Chief Scientist of Israel Ministry of Agriculture & Rural Development, PO Box 30, 50250 Bet-Dagan, Israel</affiliation><affiliation>E-mail: vhyuval@agri.gov.il</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Avihai</namePart><namePart type="family">Perl</namePart><affiliation>Institute of Plant Science, Agricultural Research Organization, 50250 Bet Dagan, Israel</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><subject><topic>Regular Papers</topic></subject><originInfo><publisher>Oxford University Press</publisher><dateIssued encoding="w3cdtf">2010-07</dateIssued><dateCreated encoding="w3cdtf">2010-06-08</dateCreated><copyrightDate encoding="w3cdtf">2010</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract>Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme’s catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a β-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</abstract><subject><genre>keywords</genre><topic>Glutathione peroxidase</topic><topic>Nicotiana tabacum</topic><topic>Reactive oxygen species</topic><topic>Redox regulation</topic><topic>Shoot regeneration</topic><topic>Transgenic plants</topic></subject><relatedItem type="host"><titleInfo><title>Plant and Cell Physiology</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">0032-0781</identifier><identifier type="eISSN">1471-9053</identifier><identifier type="PublisherID">pcp</identifier><identifier type="PublisherID-hwp">pcellphys</identifier><part><date>2010</date><detail type="volume"><caption>vol.</caption><number>51</number></detail><detail type="issue"><caption>no.</caption><number>7</number></detail><extent unit="pages"><start>1151</start><end>1162</end></extent></part></relatedItem><identifier type="istex">A4D64ABF200EC222D26122C38E3F624D8E26005C</identifier><identifier type="DOI">10.1093/pcp/pcq082</identifier><identifier type="ArticleID">pcq082</identifier><accessCondition type="use and reproduction" contentType="copyright">© The Author 2010. 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