Glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation
Identifieur interne : 000010 ( Hal/Corpus ); précédent : 000009; suivant : 000011Glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation
Auteurs : Zehava Faltin ; Doron Holland ; Margarita Velcheva ; Marina Tsapovetsky ; Patricia Roeckel-Drevet ; Avtar Handa ; Mohamad Abu-Abied ; Miriam Friedman-Einat ; Yuval Eshdat ; Avihai PerlSource :
- Plant and Cell Physiology [ 0032-0781 ] ; 2010.
English descriptors
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Abstract
Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme's catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a beta-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.
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DOI: 10.1093/pcp/pcq082
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Abu-Abied</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Friedman Einat, Miriam" sort="Friedman Einat, Miriam" uniqKey="Friedman Einat M" first="Miriam" last="Friedman-Einat">Miriam Friedman-Einat</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Eshdat, Yuval" sort="Eshdat, Yuval" uniqKey="Eshdat Y" first="Yuval" last="Eshdat">Yuval Eshdat</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation></affiliation></author><author><name sortKey="Perl, Avihai" sort="Perl, Avihai" uniqKey="Perl A" first="Avihai" last="Perl">Avihai Perl</name><affiliation><hal:affiliation type="laboratory" xml:id="struct-249046" status="INCOMING"><orgName>Institute of Plant Sciences, Volcani Center</orgName><desc><address><addrLine>Bet Dagan</addrLine><country key="IL"></country></address></desc><listRelation><relation active="#struct-359461" type="direct"></relation></listRelation><tutelles><tutelle active="#struct-359461" type="direct"><org type="institution" xml:id="struct-359461" status="INCOMING"><orgName>Institute of Plant Science</orgName><desc><address><country key="FR"></country></address></desc></org></tutelle></tutelles></hal:affiliation></affiliation></author></analytic><idno type="DOI">10.1093/pcp/pcq082</idno><series><title level="j">Plant and Cell Physiology</title><idno type="ISSN">0032-0781</idno><imprint><date type="datePub">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="mix" xml:lang="en"><term>GLUTATHIONE PEROXIDASE</term><term>REACTIVE OXYGEN SPECIES</term><term>REDOC REGULATION</term><term>SHOOT REGENERATION</term><term>TRANSGENIC PLANTS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme's catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a beta-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</div></front></TEI><hal api="V3"><titleStmt><title xml:lang="en">Glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation</title><author role="aut"><persName><forename type="first">Zehava</forename><forename type="middle">Z.</forename><surname>Faltin</surname></persName><email></email><idno type="halauthor">1002354</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Doron</forename><forename type="middle">D.</forename><surname>Holland</surname></persName><email></email><idno type="halauthor">1002355</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Margarita</forename><forename type="middle">M.</forename><surname>Velcheva</surname></persName><email></email><idno type="halauthor">1002356</idno></author><author role="aut"><persName><forename type="first">Marina</forename><forename type="middle">M.</forename><surname>Tsapovetsky</surname></persName><email></email><idno type="halauthor">1002357</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Patricia</forename><forename type="middle">P.</forename><surname>Roeckel-Drevet</surname></persName><email>Patricia.DREVET@univ-bpclermont.fr</email><idno type="halauthor">1000817</idno><affiliation ref="#struct-26029"></affiliation></author><author role="aut"><persName><forename type="first">Avtar</forename><forename type="middle">A.</forename><surname>Handa</surname></persName><email></email><idno type="halauthor">1002358</idno><affiliation ref="#struct-249048"></affiliation></author><author role="aut"><persName><forename type="first">Mohamad</forename><forename type="middle">M.</forename><surname>Abu-Abied</surname></persName><email></email><idno type="halauthor">1002359</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Miriam</forename><forename type="middle">M.</forename><surname>Friedman-Einat</surname></persName><email></email><idno type="halauthor">1002360</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Yuval</forename><forename type="middle">Y.</forename><surname>Eshdat</surname></persName><email></email><idno type="halauthor">1002361</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Avihai</forename><forename type="middle">A.</forename><surname>Perl</surname></persName><email></email><idno type="halauthor">1002362</idno><affiliation ref="#struct-249046"></affiliation></author><editor role="depositor"><persName><forename>Archive Ouverte</forename><surname>ProdInra</surname></persName><email>prodinra@inra.fr</email></editor></titleStmt><editionStmt><edition n="v1" type="current"><date type="whenSubmitted">2014-03-24 11:59:50</date><date type="whenModified">2014-03-24 11:59:50</date><date type="whenReleased">2014-03-24 11:59:50</date><date type="whenProduced">2010</date></edition><respStmt><resp>contributor</resp><name key="132775"><persName><forename>Archive Ouverte</forename><surname>ProdInra</surname></persName><email>prodinra@inra.fr</email></name></respStmt></editionStmt><publicationStmt><distributor>CCSD</distributor><idno type="halId">hal-00964726</idno><idno type="halUri">https://hal.archives-ouvertes.fr/hal-00964726</idno><idno type="halBibtex">faltin:hal-00964726</idno><idno type="halRefHtml">Plant and Cell Physiology, Oxford University Press (OUP), 2010, 51 (7), pp.1151-1162. <10.1093/pcp/pcq082></idno><idno type="halRef">Plant and Cell Physiology, Oxford University Press (OUP), 2010, 51 (7), pp.1151-1162. <10.1093/pcp/pcq082></idno></publicationStmt><seriesStmt><idno type="stamp" n="UNIV-BPCLERMONT" p="PRES_CLERMONT">Université Blaise Pascal - Clermont-Ferrand II</idno><idno type="stamp" n="PIAF" p="UNIV-BPCLERMONT">Laboratoire de Physique et Physiologie Intégratives de l'Arbre Fruitier et Forestier</idno><idno type="stamp" n="PRES_CLERMONT">Université de Clermont</idno></seriesStmt><notesStmt><note type="commentary"> </note><note type="audience" n="1">Not set</note><note type="popular" n="0">No</note><note type="peer" n="1">Yes</note></notesStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Glutathione peroxidase regulation of reactive oxygen species level is crucial for in vitro plant differentiation</title><author role="aut"><persName><forename type="first">Zehava</forename><forename type="middle">Z.</forename><surname>Faltin</surname></persName><idno type="halAuthorId">1002354</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Doron</forename><forename type="middle">D.</forename><surname>Holland</surname></persName><idno type="halAuthorId">1002355</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Margarita</forename><forename type="middle">M.</forename><surname>Velcheva</surname></persName><idno type="halAuthorId">1002356</idno></author><author role="aut"><persName><forename type="first">Marina</forename><forename type="middle">M.</forename><surname>Tsapovetsky</surname></persName><idno type="halAuthorId">1002357</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Patricia</forename><forename type="middle">P.</forename><surname>Roeckel-Drevet</surname></persName><email>Patricia.DREVET@univ-bpclermont.fr</email><idno type="halAuthorId">1000817</idno><affiliation ref="#struct-26029"></affiliation></author><author role="aut"><persName><forename type="first">Avtar</forename><forename type="middle">A.</forename><surname>Handa</surname></persName><idno type="halAuthorId">1002358</idno><affiliation ref="#struct-249048"></affiliation></author><author role="aut"><persName><forename type="first">Mohamad</forename><forename type="middle">M.</forename><surname>Abu-Abied</surname></persName><idno type="halAuthorId">1002359</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Miriam</forename><forename type="middle">M.</forename><surname>Friedman-Einat</surname></persName><idno type="halAuthorId">1002360</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Yuval</forename><forename type="middle">Y.</forename><surname>Eshdat</surname></persName><idno type="halAuthorId">1002361</idno><affiliation ref="#struct-249046"></affiliation></author><author role="aut"><persName><forename type="first">Avihai</forename><forename type="middle">A.</forename><surname>Perl</surname></persName><idno type="halAuthorId">1002362</idno><affiliation ref="#struct-249046"></affiliation></author></analytic><monogr><idno type="halJournalId" status="VALID">7821</idno><idno type="issn">0032-0781</idno><idno type="eissn">1471-9053</idno><title level="j">Plant and Cell Physiology</title><imprint><publisher>Oxford University Press (OUP)</publisher><biblScope unit="volume">51</biblScope><biblScope unit="issue">7</biblScope><biblScope unit="pp">1151-1162</biblScope><date type="datePub">2010</date></imprint></monogr><idno type="doi">10.1093/pcp/pcq082</idno></biblStruct></sourceDesc><profileDesc><langUsage><language ident="en">English</language></langUsage><textClass><keywords scheme="author"><term xml:lang="en">GLUTATHIONE PEROXIDASE</term><term xml:lang="en">REACTIVE OXYGEN SPECIES</term><term xml:lang="en">REDOC REGULATION</term><term xml:lang="en">SHOOT REGENERATION</term><term xml:lang="en">TRANSGENIC PLANTS</term></keywords><classCode scheme="halDomain" n="sdv.sa">Life Sciences [q-bio]/Agricultural sciences</classCode><classCode scheme="halTypology" n="ART">Journal articles</classCode></textClass><abstract xml:lang="en">Phospholipid hydroperoxide glutathione peroxidase (PHGPx) is overexpressed in plants under abiotic and biotic stress conditions that mediate oxidative stress. To study its biological role and its ability to confer stress resistance in plants, we tried to obtain transgenic plants overexpressing citrus (Citrus sinensis) PHGPx (cit-PHGPx). All attempts to obtain regenerated plants expressing this enzyme constitutively failed. However, when the enzyme's catalytic activity was abolished by active site-directed mutagenesis, transgenic plants constitutively expressing inactive cit-PHGPx were successfully regenerated. Constitutive expression of enzymatically active cit-PHGPx could only be obtained when transformation was based on non-regenerative processes. These results indicate that overexpression of the antioxidant enzyme PHGPx interferes with shoot organogenesis and suggests the involvement of reactive oxygen species (ROS) in this process. Using transgenic tobacco (Nicotiana tabacum) leaves obtained from plants transformed with a beta-estradiol-inducible promoter, time-dependent induction of cit-PHGPx expression was employed. A pronounced inhibitory effect of cit-PHGPx on shoot formation was found to be limited to the early stage of the regeneration process. Monitoring the ROS level during regeneration revealed that upon cit-PHGPx induction, the lowest level of ROS correlated with the maximal level of shoot inhibition. Our results clearly demonstrate the essential role of ROS in the early stages of in vitro shoot organogenesis and the possible involvement of PHGPx in maintaining ROS homeostasis at this point.</abstract></profileDesc></hal></record>Pour manipuler ce document sous Unix (Dilib)
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