Characterization of major anthocyanins and the color of red-fleshed budd blood orange (Citrus sinensis)
Identifieur interne : 002C78 ( Main/Exploration ); précédent : 002C77; suivant : 002C79Characterization of major anthocyanins and the color of red-fleshed budd blood orange (Citrus sinensis)
Auteurs : Hyoung S. Lee [États-Unis]Source :
- Journal of agricultural and food chemistry : (Print) [ 0021-8561 ] ; 2002.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
- Anthocyanin, Anthocyanins (analysis), Anthocyanins (chemistry), Blood orange, Characterization, Chemical composition, Chromatography, High Pressure Liquid (methods), Citrus (chemistry), Citrus sinensis, Color, Cultivar, Food Coloring Agents (analysis), Identification, Intraspecific comparison, Malonic acid, Orange juice, Pigments, Biological (analysis), Pigments, Biological (chemistry), Species Specificity, Spectrophotometry (methods).
- MESH :
- chemical , analysis : Anthocyanins, Food Coloring Agents, Pigments, Biological.
- chemical , chemistry : Anthocyanins, Pigments, Biological.
- chemistry : Citrus.
- methods : Chromatography, High Pressure Liquid, Spectrophotometry.
- Species Specificity.
Abstract
High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H3PO4 in water and 0.1% H3PO4 in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-μm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L*,a*,b*) were compared with six other Florida-grown blood oranges.
Affiliations:
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Le document en format XML
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Lee</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research & Education Center, Florida Department of Citrus, 700 Experiment Station Road</s1><s2>Lake Alfred, Florida 33850</s2><s3>USA</s3><sZ>1 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">02-0203885</idno><date when="2002">2002</date><idno type="stanalyst">PASCAL 02-0203885 INIST</idno><idno type="RBID">Pascal:02-0203885</idno><idno type="wicri:Area/PascalFrancis/Corpus">000846</idno><idno type="wicri:Area/PascalFrancis/Curation">000158</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000818</idno><idno type="wicri:doubleKey">0021-8561:2002:Lee H:characterization:of:major</idno><idno type="wicri:Area/Main/Merge">002E57</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:11853511</idno><idno type="wicri:Area/PubMed/Corpus">000E81</idno><idno type="wicri:Area/PubMed/Curation">000E81</idno><idno type="wicri:Area/PubMed/Checkpoint">000E81</idno><idno type="wicri:Area/Ncbi/Merge">000045</idno><idno type="wicri:Area/Ncbi/Curation">000045</idno><idno type="wicri:Area/Ncbi/Checkpoint">000045</idno><idno type="wicri:doubleKey">0021-8561:2002:Lee H:characterization:of:major</idno><idno type="wicri:Area/Main/Merge">002D65</idno><idno type="wicri:Area/Main/Curation">002C78</idno><idno type="wicri:Area/Main/Exploration">002C78</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Characterization of major anthocyanins and the color of red-fleshed budd blood orange (Citrus sinensis)</title><author><name sortKey="Lee, Hyoung S" sort="Lee, Hyoung S" uniqKey="Lee H" first="Hyoung S." last="Lee">Hyoung S. Lee</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Citrus Research & Education Center, Florida Department of Citrus, 700 Experiment Station Road</s1><s2>Lake Alfred, Florida 33850</s2><s3>USA</s3><sZ>1 aut.</sZ></inist:fA14><country>États-Unis</country><wicri:noRegion>Lake Alfred, Florida 33850</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Journal of agricultural and food chemistry : (Print)</title><title level="j" type="abbreviated">J. agric. food chem. : (Print)</title><idno type="ISSN">0021-8561</idno><imprint><date when="2002">2002</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Journal of agricultural and food chemistry : (Print)</title><title level="j" type="abbreviated">J. agric. food chem. : (Print)</title><idno type="ISSN">0021-8561</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anthocyanin</term><term>Anthocyanins (analysis)</term><term>Anthocyanins (chemistry)</term><term>Blood orange</term><term>Characterization</term><term>Chemical composition</term><term>Chromatography, High Pressure Liquid (methods)</term><term>Citrus (chemistry)</term><term>Citrus sinensis</term><term>Color</term><term>Cultivar</term><term>Food Coloring Agents (analysis)</term><term>Identification</term><term>Intraspecific comparison</term><term>Malonic acid</term><term>Orange juice</term><term>Pigments, Biological (analysis)</term><term>Pigments, Biological (chemistry)</term><term>Species Specificity</term><term>Spectrophotometry (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Anthocyanins</term><term>Food Coloring Agents</term><term>Pigments, Biological</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anthocyanins</term><term>Pigments, Biological</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Spectrophotometry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Species Specificity</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Caractérisation</term><term>Composition chimique</term><term>Couleur</term><term>Jus d'orange</term><term>Identification</term><term>Comparaison intraspécifique</term><term>Cultivar</term><term>Citrus sinensis</term><term>Anthocyane</term><term>Malonique acide</term><term>Flavylium(3,3p,4p,5,7-pentahydroxy)</term><term>Orange sanguine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H<sub>3</sub>PO<sub>4</sub> in water and 0.1% H<sub>3</sub>PO<sub>4</sub> in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-μm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L<sup>*</sup>,a<sup>*</sup>,b<sup>*</sup>) were compared with six other Florida-grown blood oranges.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Lee, Hyoung S" sort="Lee, Hyoung S" uniqKey="Lee H" first="Hyoung S." last="Lee">Hyoung S. Lee</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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