Characterization of major anthocyanins and the color of red-fleshed Budd Blood orange (Citrus sinensis).
Identifieur interne : 000045 ( Ncbi/Merge ); précédent : 000044; suivant : 000046Characterization of major anthocyanins and the color of red-fleshed Budd Blood orange (Citrus sinensis).
Auteurs : Hyoung S. Lee [États-Unis]Source :
- Journal of agricultural and food chemistry [ 0021-8561 ] ; 2002.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Anthocyanins, Food Coloring Agents, Pigments, Biological.
- chemical , chemistry : Anthocyanins, Pigments, Biological.
- chemistry : Citrus.
- methods : Chromatography, High Pressure Liquid, Spectrophotometry.
- Species Specificity.
Abstract
High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H(3)P0(4) in water and 0.1% H(3)PO(4) in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-microm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L,a,b) were compared with six other Florida-grown blood oranges.
PubMed: 11853511
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Lee</name><affiliation wicri:level="2"><nlm:affiliation>Citrus Research & Education Center, Florida Department of Citrus, 700 Experiment Station Road, Lake Alfred, FL 33850, USA. hsl@lal.ufl.edu</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Citrus Research & Education Center, Florida Department of Citrus, 700 Experiment Station Road, Lake Alfred, FL 33850</wicri:regionArea><placeName><region type="state">Floride</region></placeName></affiliation></author></analytic><series><title level="j">Journal of agricultural and food chemistry</title><idno type="ISSN">0021-8561</idno><imprint><date when="2002" type="published">2002</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anthocyanins (analysis)</term><term>Anthocyanins (chemistry)</term><term>Chromatography, High Pressure Liquid (methods)</term><term>Citrus (chemistry)</term><term>Food Coloring Agents (analysis)</term><term>Pigments, Biological (analysis)</term><term>Pigments, Biological (chemistry)</term><term>Species Specificity</term><term>Spectrophotometry (methods)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Anthocyanins</term><term>Food Coloring Agents</term><term>Pigments, Biological</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Anthocyanins</term><term>Pigments, Biological</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Citrus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Spectrophotometry</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Species Specificity</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H(3)P0(4) in water and 0.1% H(3)PO(4) in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-microm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L,a,b) were compared with six other Florida-grown blood oranges.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">11853511</PMID><DateCreated><Year>2002</Year><Month>2</Month><Day>20</Day></DateCreated><DateCompleted><Year>2002</Year><Month>05</Month><Day>02</Day></DateCompleted><DateRevised><Year>2005</Year><Month>11</Month><Day>17</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0021-8561</ISSN><JournalIssue CitedMedium="Print"><Volume>50</Volume><Issue>5</Issue><PubDate><Year>2002</Year><Month>Feb</Month><Day>27</Day></PubDate></JournalIssue><Title>Journal of agricultural and food chemistry</Title><ISOAbbreviation>J. Agric. Food Chem.</ISOAbbreviation></Journal><ArticleTitle>Characterization of major anthocyanins and the color of red-fleshed Budd Blood orange (Citrus sinensis).</ArticleTitle><Pagination><MedlinePgn>1243-6</MedlinePgn></Pagination><Abstract><AbstractText>High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H(3)P0(4) in water and 0.1% H(3)PO(4) in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-microm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. 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