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Characterization of major anthocyanins and the color of red-fleshed budd blood orange (Citrus sinensis)

Identifieur interne : 000846 ( PascalFrancis/Corpus ); précédent : 000845; suivant : 000847

Characterization of major anthocyanins and the color of red-fleshed budd blood orange (Citrus sinensis)

Auteurs : Hyoung S. Lee

Source :

RBID : Pascal:02-0203885

Descripteurs français

English descriptors

Abstract

High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H3PO4 in water and 0.1% H3PO4 in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-μm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L*,a*,b*) were compared with six other Florida-grown blood oranges.

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 0021-8561
A02 01      @0 JAFCAU
A03   1    @0 J. agric. food chem. : (Print)
A05       @2 50
A06       @2 5
A08 01  1  ENG  @1 Characterization of major anthocyanins and the color of red-fleshed budd blood orange (Citrus sinensis)
A11 01  1    @1 LEE (Hyoung S.)
A14 01      @1 Citrus Research & Education Center, Florida Department of Citrus, 700 Experiment Station Road @2 Lake Alfred, Florida 33850 @3 USA @Z 1 aut.
A20       @1 1243-1246
A21       @1 2002
A23 01      @0 ENG
A43 01      @1 INIST @2 7332 @5 354000100288100490
A44       @0 0000 @1 © 2002 INIST-CNRS. All rights reserved.
A45       @0 25 ref.
A47 01  1    @0 02-0203885
A60       @1 P
A61       @0 A
A64 01  1    @0 Journal of agricultural and food chemistry : (Print)
A66 01      @0 USA
C01 01    ENG  @0 High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H3PO4 in water and 0.1% H3PO4 in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-μm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L*,a*,b*) were compared with six other Florida-grown blood oranges.
C02 01  X    @0 002A35B09
C02 02  X    @0 002A32E03
C03 01  X  FRE  @0 Caractérisation @5 01
C03 01  X  ENG  @0 Characterization @5 01
C03 01  X  SPA  @0 Caracterización @5 01
C03 02  X  FRE  @0 Composition chimique @5 02
C03 02  X  ENG  @0 Chemical composition @5 02
C03 02  X  SPA  @0 Composición química @5 02
C03 03  X  FRE  @0 Couleur @5 03
C03 03  X  ENG  @0 Color @5 03
C03 03  X  SPA  @0 Color @5 03
C03 04  X  FRE  @0 Jus d'orange @5 04
C03 04  X  ENG  @0 Orange juice @5 04
C03 04  X  SPA  @0 Zugo naranja @5 04
C03 05  X  FRE  @0 Identification @5 05
C03 05  X  ENG  @0 Identification @5 05
C03 05  X  SPA  @0 Identificación @5 05
C03 06  X  FRE  @0 Comparaison intraspécifique @5 06
C03 06  X  ENG  @0 Intraspecific comparison @5 06
C03 06  X  SPA  @0 Comparación intraespecífica @5 06
C03 07  X  FRE  @0 Cultivar @5 07
C03 07  X  ENG  @0 Cultivar @5 07
C03 07  X  SPA  @0 Cultivar @5 07
C03 08  X  FRE  @0 Citrus sinensis @2 NS @5 10
C03 08  X  ENG  @0 Citrus sinensis @2 NS @5 10
C03 08  X  SPA  @0 Citrus sinensis @2 NS @5 10
C03 09  X  FRE  @0 Anthocyane @5 15
C03 09  X  ENG  @0 Anthocyanin @5 15
C03 09  X  SPA  @0 Antocianina @5 15
C03 10  X  FRE  @0 Malonique acide @2 NK @5 17
C03 10  X  ENG  @0 Malonic acid @2 NK @5 17
C03 10  X  SPA  @0 Malónico ácido @2 NK @5 17
C03 11  X  FRE  @0 Flavylium(3,3p,4p,5,7-pentahydroxy) @2 FF @4 INC @5 82 @6 Flavylium(«3,3p,4p,5,7»-pentahydroxy)
C03 12  X  FRE  @0 Orange sanguine @4 CD @5 96
C03 12  X  ENG  @0 Blood orange @4 CD @5 96
C07 01  X  FRE  @0 Rutaceae @2 NS
C07 01  X  ENG  @0 Rutaceae @2 NS
C07 01  X  SPA  @0 Rutaceae @2 NS
C07 02  X  FRE  @0 Dicotyledones @2 NS
C07 02  X  ENG  @0 Dicotyledones @2 NS
C07 02  X  SPA  @0 Dicotyledones @2 NS
C07 03  X  FRE  @0 Angiospermae @2 NS
C07 03  X  ENG  @0 Angiospermae @2 NS
C07 03  X  SPA  @0 Angiospermae @2 NS
C07 04  X  FRE  @0 Spermatophyta @2 NS
C07 04  X  ENG  @0 Spermatophyta @2 NS
C07 04  X  SPA  @0 Spermatophyta @2 NS
C07 05  X  FRE  @0 Agrume @5 39
C07 05  X  ENG  @0 Citrus fruit @5 39
C07 05  X  SPA  @0 Agrios @5 39
C07 06  X  FRE  @0 Flavonoïde @5 50
C07 06  X  ENG  @0 Flavonoid @5 50
C07 06  X  SPA  @0 Flavonoide @5 50
N21       @1 119
N82       @1 PSI

Format Inist (serveur)

NO : PASCAL 02-0203885 INIST
ET : Characterization of major anthocyanins and the color of red-fleshed budd blood orange (Citrus sinensis)
AU : LEE (Hyoung S.)
AF : Citrus Research & Education Center, Florida Department of Citrus, 700 Experiment Station Road/Lake Alfred, Florida 33850/Etats-Unis (1 aut.)
DT : Publication en série; Niveau analytique
SO : Journal of agricultural and food chemistry : (Print); ISSN 0021-8561; Coden JAFCAU; Etats-Unis; Da. 2002; Vol. 50; No. 5; Pp. 1243-1246; Bibl. 25 ref.
LA : Anglais
EA : High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H3PO4 in water and 0.1% H3PO4 in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-μm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L*,a*,b*) were compared with six other Florida-grown blood oranges.
CC : 002A35B09; 002A32E03
FD : Caractérisation; Composition chimique; Couleur; Jus d'orange; Identification; Comparaison intraspécifique; Cultivar; Citrus sinensis; Anthocyane; Malonique acide; Flavylium(3,3p,4p,5,7-pentahydroxy); Orange sanguine
FG : Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Flavonoïde
ED : Characterization; Chemical composition; Color; Orange juice; Identification; Intraspecific comparison; Cultivar; Citrus sinensis; Anthocyanin; Malonic acid; Blood orange
EG : Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Flavonoid
SD : Caracterización; Composición química; Color; Zugo naranja; Identificación; Comparación intraespecífica; Cultivar; Citrus sinensis; Antocianina; Malónico ácido
LO : INIST-7332.354000100288100490
ID : 02-0203885

Links to Exploration step

Pascal:02-0203885

Le document en format XML

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<div type="abstract" xml:lang="en">High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H
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PO
<sub>4</sub>
in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-μm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L
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,a
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,b
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<s0>High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H
<sub>3</sub>
PO
<sub>4</sub>
in water and 0.1% H
<sub>3</sub>
PO
<sub>4</sub>
in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-μm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L
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<s5>03</s5>
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<fC03 i1="03" i2="X" l="ENG">
<s0>Color</s0>
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<fC03 i1="03" i2="X" l="SPA">
<s0>Color</s0>
<s5>03</s5>
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<fC03 i1="04" i2="X" l="FRE">
<s0>Jus d'orange</s0>
<s5>04</s5>
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<s5>05</s5>
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<fC03 i1="05" i2="X" l="ENG">
<s0>Identification</s0>
<s5>05</s5>
</fC03>
<fC03 i1="05" i2="X" l="SPA">
<s0>Identificación</s0>
<s5>05</s5>
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<fC03 i1="06" i2="X" l="FRE">
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<s0>Cultivar</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="ENG">
<s0>Cultivar</s0>
<s5>07</s5>
</fC03>
<fC03 i1="07" i2="X" l="SPA">
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<s5>07</s5>
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<s2>FF</s2>
<s4>INC</s4>
<s5>82</s5>
<s6>Flavylium(«3,3p,4p,5,7»-pentahydroxy)</s6>
</fC03>
<fC03 i1="12" i2="X" l="FRE">
<s0>Orange sanguine</s0>
<s4>CD</s4>
<s5>96</s5>
</fC03>
<fC03 i1="12" i2="X" l="ENG">
<s0>Blood orange</s0>
<s4>CD</s4>
<s5>96</s5>
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<fC07 i1="01" i2="X" l="FRE">
<s0>Rutaceae</s0>
<s2>NS</s2>
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<fC07 i1="01" i2="X" l="ENG">
<s0>Rutaceae</s0>
<s2>NS</s2>
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<fC07 i1="01" i2="X" l="SPA">
<s0>Rutaceae</s0>
<s2>NS</s2>
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<fC07 i1="02" i2="X" l="FRE">
<s0>Dicotyledones</s0>
<s2>NS</s2>
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<fC07 i1="02" i2="X" l="ENG">
<s0>Dicotyledones</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="02" i2="X" l="SPA">
<s0>Dicotyledones</s0>
<s2>NS</s2>
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<fC07 i1="03" i2="X" l="FRE">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="ENG">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="03" i2="X" l="SPA">
<s0>Angiospermae</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="FRE">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="ENG">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="04" i2="X" l="SPA">
<s0>Spermatophyta</s0>
<s2>NS</s2>
</fC07>
<fC07 i1="05" i2="X" l="FRE">
<s0>Agrume</s0>
<s5>39</s5>
</fC07>
<fC07 i1="05" i2="X" l="ENG">
<s0>Citrus fruit</s0>
<s5>39</s5>
</fC07>
<fC07 i1="05" i2="X" l="SPA">
<s0>Agrios</s0>
<s5>39</s5>
</fC07>
<fC07 i1="06" i2="X" l="FRE">
<s0>Flavonoïde</s0>
<s5>50</s5>
</fC07>
<fC07 i1="06" i2="X" l="ENG">
<s0>Flavonoid</s0>
<s5>50</s5>
</fC07>
<fC07 i1="06" i2="X" l="SPA">
<s0>Flavonoide</s0>
<s5>50</s5>
</fC07>
<fN21>
<s1>119</s1>
</fN21>
<fN82>
<s1>PSI</s1>
</fN82>
</pA>
</standard>
<server>
<NO>PASCAL 02-0203885 INIST</NO>
<ET>Characterization of major anthocyanins and the color of red-fleshed budd blood orange (Citrus sinensis)</ET>
<AU>LEE (Hyoung S.)</AU>
<AF>Citrus Research & Education Center, Florida Department of Citrus, 700 Experiment Station Road/Lake Alfred, Florida 33850/Etats-Unis (1 aut.)</AF>
<DT>Publication en série; Niveau analytique</DT>
<SO>Journal of agricultural and food chemistry : (Print); ISSN 0021-8561; Coden JAFCAU; Etats-Unis; Da. 2002; Vol. 50; No. 5; Pp. 1243-1246; Bibl. 25 ref.</SO>
<LA>Anglais</LA>
<EA>High-performance liquid chromatography (HPLC) with photodiode array detection was applied for the characterization of anthocyanins in red-fleshed Budd Blood (Citrus sinensis) orange. More than seven anthocyanin pigments were separated within 30 min by using a binary gradient (0.1% H
<sub>3</sub>
PO
<sub>4</sub>
in water and 0.1% H
<sub>3</sub>
PO
<sub>4</sub>
in acetonitrile) elution on a Prodigy ODS column. Separations by reversed-phase HPLC and semipreparative HPLC on a Prodigy 10-μm ODS Prep column, and acid and alkali hydrolyses were used for identification of anthocyanins. The primary anthocyanins in Budd Blood orange grown in Florida were cyanidin-3-(6"-malonylglucoside) (44.8%) followed by cyanidin-3-glucoside (33.6%). Two other minor pigments were also acylated with malonic acid. Malonated anthocyanins represented the major proportion (>51%) of anthocyanins in Budd Blood orange. Total anthocyanin contents and juice color parameters (CIE L
<sup>*</sup>
,a
<sup>*</sup>
,b
<sup>*</sup>
) were compared with six other Florida-grown blood oranges.</EA>
<CC>002A35B09; 002A32E03</CC>
<FD>Caractérisation; Composition chimique; Couleur; Jus d'orange; Identification; Comparaison intraspécifique; Cultivar; Citrus sinensis; Anthocyane; Malonique acide; Flavylium(3,3p,4p,5,7-pentahydroxy); Orange sanguine</FD>
<FG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Agrume; Flavonoïde</FG>
<ED>Characterization; Chemical composition; Color; Orange juice; Identification; Intraspecific comparison; Cultivar; Citrus sinensis; Anthocyanin; Malonic acid; Blood orange</ED>
<EG>Rutaceae; Dicotyledones; Angiospermae; Spermatophyta; Citrus fruit; Flavonoid</EG>
<SD>Caracterización; Composición química; Color; Zugo naranja; Identificación; Comparación intraespecífica; Cultivar; Citrus sinensis; Antocianina; Malónico ácido</SD>
<LO>INIST-7332.354000100288100490</LO>
<ID>02-0203885</ID>
</server>
</inist>
</record>

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