Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis
Identifieur interne : 003120 ( Main/Exploration ); précédent : 003119; suivant : 003121Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis
Auteurs : JIHONG LIU [République populaire de Chine] ; XIUXIN DENG [République populaire de Chine]Source :
- Plant cell, tissue and organ culture [ 0167-6857 ] ; 1999.
Descripteurs français
- Pascal (Inist)
- Wicri :
- topic : Production, Agrume.
English descriptors
- KwdEn :
Abstract
X-ray irradiated embryogenic protoplasts of Microcitrus papuana Swing, were electrically fused with iodoacetic acid-treated embryogenic protoplasts of Newhall navel orange [Citrus sinensis (L.) Osb.]. Seven cell lines were established by low-melting agarose embedding culture of fusion-treated protoplasts. Cytological examination of 4 cell lines showed that each cell line consisted of many aneuploid (45.10%, 38.98%, 32.69% and 34.85%, respectively) and diploid cells (52.94%, 59.33%, 63.46% and 62.12%, respectively), whereas only a few tetraploid cells (1.96%, 1.69%, 3.85% and 3.03%, respectively) were detected. Analyses of random amplified polymorphic DNA with four 10-mer primers confirmed the hybrid characteristics of the cell lines, which in combination with chromosome counting proved that the cell lines were asymmetric hybrids.
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 000947
- to stream PascalFrancis, to step Curation: 000057
- to stream PascalFrancis, to step Checkpoint: 000994
- to stream Main, to step Merge: 003349
- to stream Main, to step Curation: 003120
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis</title><author><name sortKey="Jihong Liu" sort="Jihong Liu" uniqKey="Jihong Liu" last="Jihong Liu">JIHONG LIU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Xiuxin Deng" sort="Xiuxin Deng" uniqKey="Xiuxin Deng" last="Xiuxin Deng">XIUXIN DENG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">INIST</idno><idno type="inist">00-0520429</idno><date when="1999">1999</date><idno type="stanalyst">PASCAL 00-0520429 INIST</idno><idno type="RBID">Pascal:00-0520429</idno><idno type="wicri:Area/PascalFrancis/Corpus">000947</idno><idno type="wicri:Area/PascalFrancis/Curation">000057</idno><idno type="wicri:Area/PascalFrancis/Checkpoint">000994</idno><idno type="wicri:doubleKey">0167-6857:1999:Jihong Liu:production:of:hybrid</idno><idno type="wicri:Area/Main/Merge">003349</idno><idno type="wicri:Area/Main/Curation">003120</idno><idno type="wicri:Area/Main/Exploration">003120</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis</title><author><name sortKey="Jihong Liu" sort="Jihong Liu" uniqKey="Jihong Liu" last="Jihong Liu">JIHONG LIU</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author><author><name sortKey="Xiuxin Deng" sort="Xiuxin Deng" uniqKey="Xiuxin Deng" last="Xiuxin Deng">XIUXIN DENG</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1><s2>Wuhan 430070</s2><s3>CHN</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ></inist:fA14><country>République populaire de Chine</country><wicri:noRegion>Wuhan 430070</wicri:noRegion></affiliation></author></analytic><series><title level="j" type="main">Plant cell, tissue and organ culture</title><title level="j" type="abbreviated">Plant cell tissue organ cult.</title><idno type="ISSN">0167-6857</idno><imprint><date when="1999">1999</date></imprint></series></biblStruct></sourceDesc><seriesStmt><title level="j" type="main">Plant cell, tissue and organ culture</title><title level="j" type="abbreviated">Plant cell tissue organ cult.</title><idno type="ISSN">0167-6857</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Callogenesis</term><term>Citrus fruit</term><term>Citrus sinensis</term><term>Genetic marker</term><term>Hybrid</term><term>Production</term><term>Protoplast</term><term>Random amplified polymorphic DNA marker</term><term>Tissue culture</term><term>Vegetals</term><term>X ray irradiation</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Production</term><term>Hybride</term><term>Culture tissu</term><term>Végétal</term><term>Callogenèse</term><term>Citrus sinensis</term><term>Irradiation RX</term><term>Marqueur génétique</term><term>Marqueur RAPD</term><term>Agrume</term><term>Protoplaste</term><term>Microcitrus papuana</term></keywords><keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Production</term><term>Agrume</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">X-ray irradiated embryogenic protoplasts of Microcitrus papuana Swing, were electrically fused with iodoacetic acid-treated embryogenic protoplasts of Newhall navel orange [Citrus sinensis (L.) Osb.]. Seven cell lines were established by low-melting agarose embedding culture of fusion-treated protoplasts. Cytological examination of 4 cell lines showed that each cell line consisted of many aneuploid (45.10%, 38.98%, 32.69% and 34.85%, respectively) and diploid cells (52.94%, 59.33%, 63.46% and 62.12%, respectively), whereas only a few tetraploid cells (1.96%, 1.69%, 3.85% and 3.03%, respectively) were detected. Analyses of random amplified polymorphic DNA with four 10-mer primers confirmed the hybrid characteristics of the cell lines, which in combination with chromosome counting proved that the cell lines were asymmetric hybrids.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Jihong Liu" sort="Jihong Liu" uniqKey="Jihong Liu" last="Jihong Liu">JIHONG LIU</name></noRegion><name sortKey="Xiuxin Deng" sort="Xiuxin Deng" uniqKey="Xiuxin Deng" last="Xiuxin Deng">XIUXIN DENG</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003120 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003120 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Bois
|area= OrangerV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= Pascal:00-0520429
|texte= Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis
}}
| This area was generated with Dilib version V0.6.25. |  |