OrangerV1, Main, Merge, bibRecord, 003349

Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis

Identifieur interne : 003349 ( Main/Merge ); précédent : 003348; suivant : 003350

Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis

Auteurs : JIHONG LIU [République populaire de Chine] ; XIUXIN DENG [République populaire de Chine]

Source :

Plant cell, tissue and organ culture [ 0167-6857 ] ; 1999.

RBID : Pascal:00-0520429

Descripteurs français

Pascal (Inist)
- Production, Hybride, Culture tissu, Végétal, Callogenèse, Citrus sinensis, Irradiation RX, Marqueur génétique, Marqueur RAPD, Agrume, Protoplaste, Microcitrus papuana.
Wicri :
- topic : Production, Agrume.

English descriptors

KwdEn :
- Callogenesis, Citrus fruit, Citrus sinensis, Genetic marker, Hybrid, Production, Protoplast, Random amplified polymorphic DNA marker, Tissue culture, Vegetals, X ray irradiation.

Abstract

X-ray irradiated embryogenic protoplasts of Microcitrus papuana Swing, were electrically fused with iodoacetic acid-treated embryogenic protoplasts of Newhall navel orange [Citrus sinensis (L.) Osb.]. Seven cell lines were established by low-melting agarose embedding culture of fusion-treated protoplasts. Cytological examination of 4 cell lines showed that each cell line consisted of many aneuploid (45.10%, 38.98%, 32.69% and 34.85%, respectively) and diploid cells (52.94%, 59.33%, 63.46% and 62.12%, respectively), whereas only a few tetraploid cells (1.96%, 1.69%, 3.85% and 3.03%, respectively) were detected. Analyses of random amplified polymorphic DNA with four 10-mer primers confirmed the hybrid characteristics of the cell lines, which in combination with chromosome counting proved that the cell lines were asymmetric hybrids.

Links toward previous steps (curation, corpus...)

to stream PascalFrancis, to step Corpus: 000947
to stream PascalFrancis, to step Curation: 000057
to stream PascalFrancis, to step Checkpoint: 000994

Links to Exploration step

Pascal:00-0520429

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis</title>
<author><name sortKey="Jihong Liu" sort="Jihong Liu" uniqKey="Jihong Liu" last="Jihong Liu">JIHONG LIU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1>
<s2>Wuhan 430070</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Wuhan 430070</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xiuxin Deng" sort="Xiuxin Deng" uniqKey="Xiuxin Deng" last="Xiuxin Deng">XIUXIN DENG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1>
<s2>Wuhan 430070</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Wuhan 430070</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">00-0520429</idno>
<date when="1999">1999</date>
<idno type="stanalyst">PASCAL 00-0520429 INIST</idno>
<idno type="RBID">Pascal:00-0520429</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">000947</idno>
<idno type="wicri:Area/PascalFrancis/Curation">000057</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">000994</idno>
<idno type="wicri:doubleKey">0167-6857:1999:Jihong Liu:production:of:hybrid</idno>
<idno type="wicri:Area/Main/Merge">003349</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis</title>
<author><name sortKey="Jihong Liu" sort="Jihong Liu" uniqKey="Jihong Liu" last="Jihong Liu">JIHONG LIU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1>
<s2>Wuhan 430070</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Wuhan 430070</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Xiuxin Deng" sort="Xiuxin Deng" uniqKey="Xiuxin Deng" last="Xiuxin Deng">XIUXIN DENG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1>
<s2>Wuhan 430070</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
</inist:fA14>
<country>République populaire de Chine</country>
<wicri:noRegion>Wuhan 430070</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Plant cell, tissue and organ culture</title>
<title level="j" type="abbreviated">Plant cell tissue organ cult.</title>
<idno type="ISSN">0167-6857</idno>
<imprint><date when="1999">1999</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Plant cell, tissue and organ culture</title>
<title level="j" type="abbreviated">Plant cell tissue organ cult.</title>
<idno type="ISSN">0167-6857</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Callogenesis</term>
<term>Citrus fruit</term>
<term>Citrus sinensis</term>
<term>Genetic marker</term>
<term>Hybrid</term>
<term>Production</term>
<term>Protoplast</term>
<term>Random amplified polymorphic DNA marker</term>
<term>Tissue culture</term>
<term>Vegetals</term>
<term>X ray irradiation</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Production</term>
<term>Hybride</term>
<term>Culture tissu</term>
<term>Végétal</term>
<term>Callogenèse</term>
<term>Citrus sinensis</term>
<term>Irradiation RX</term>
<term>Marqueur génétique</term>
<term>Marqueur RAPD</term>
<term>Agrume</term>
<term>Protoplaste</term>
<term>Microcitrus papuana</term>
</keywords>
<keywords scheme="Wicri" type="topic" xml:lang="fr"><term>Production</term>
<term>Agrume</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">X-ray irradiated embryogenic protoplasts of Microcitrus papuana Swing, were electrically fused with iodoacetic acid-treated embryogenic protoplasts of Newhall navel orange [Citrus sinensis (L.) Osb.]. Seven cell lines were established by low-melting agarose embedding culture of fusion-treated protoplasts. Cytological examination of 4 cell lines showed that each cell line consisted of many aneuploid (45.10%, 38.98%, 32.69% and 34.85%, respectively) and diploid cells (52.94%, 59.33%, 63.46% and 62.12%, respectively), whereas only a few tetraploid cells (1.96%, 1.69%, 3.85% and 3.03%, respectively) were detected. Analyses of random amplified polymorphic DNA with four 10-mer primers confirmed the hybrid characteristics of the cell lines, which in combination with chromosome counting proved that the cell lines were asymmetric hybrids.</div>
</front>
</TEI>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><country name="République populaire de Chine"><noRegion><name sortKey="Jihong Liu" sort="Jihong Liu" uniqKey="Jihong Liu" last="Jihong Liu">JIHONG LIU</name>
</noRegion>
<name sortKey="Xiuxin Deng" sort="Xiuxin Deng" uniqKey="Xiuxin Deng" last="Xiuxin Deng">XIUXIN DENG</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Wicri/Bois/explor/OrangerV1/Data/Main/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003349 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 003349 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Wicri/Bois
   |area=    OrangerV1
   |flux=    Main
   |étape=   Merge
   |type=    RBID
   |clé=     Pascal:00-0520429
   |texte=   Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis
}}

This area was generated with Dilib version V0.6.25.
Data generation: Sat Dec 3 17:11:04 2016. Site generation: Wed Mar 6 18:18:32 2024

	Serveur d'exploration sur l'oranger
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur l'oranger

Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis

Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri