OrangerV1, PascalFrancis, Checkpoint, bibRecord, 000994

Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis

Identifieur interne : 000994 ( PascalFrancis/Checkpoint ); précédent : 000993; suivant : 000995

Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis

Auteurs : JIHONG LIU [République populaire de Chine] ; XIUXIN DENG [République populaire de Chine]

Source :

Plant cell, tissue and organ culture [ 0167-6857 ] ; 1999.

RBID : Pascal:00-0520429

Descripteurs français

Pascal (Inist)
- Production, Hybride, Culture tissu, Végétal, Callogenèse, Citrus sinensis, Irradiation RX, Marqueur génétique, Marqueur RAPD, Agrume, Protoplaste, Microcitrus papuana.
Wicri :
- topic : Production, Agrume.

English descriptors

KwdEn :
- Callogenesis, Citrus fruit, Citrus sinensis, Genetic marker, Hybrid, Production, Protoplast, Random amplified polymorphic DNA marker, Tissue culture, Vegetals, X ray irradiation.

Abstract

X-ray irradiated embryogenic protoplasts of Microcitrus papuana Swing, were electrically fused with iodoacetic acid-treated embryogenic protoplasts of Newhall navel orange [Citrus sinensis (L.) Osb.]. Seven cell lines were established by low-melting agarose embedding culture of fusion-treated protoplasts. Cytological examination of 4 cell lines showed that each cell line consisted of many aneuploid (45.10%, 38.98%, 32.69% and 34.85%, respectively) and diploid cells (52.94%, 59.33%, 63.46% and 62.12%, respectively), whereas only a few tetraploid cells (1.96%, 1.69%, 3.85% and 3.03%, respectively) were detected. Analyses of random amplified polymorphic DNA with four 10-mer primers confirmed the hybrid characteristics of the cell lines, which in combination with chromosome counting proved that the cell lines were asymmetric hybrids.

Affiliations:

République populaire de Chine

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Pascal:00-0520429

Le document en format XML

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<author><name sortKey="Jihong Liu" sort="Jihong Liu" uniqKey="Jihong Liu" last="Jihong Liu">JIHONG LIU</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1>
<s2>Wuhan 430070</s2>
<s3>CHN</s3>
<sZ>1 aut.</sZ>
<sZ>2 aut.</sZ>
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<country>République populaire de Chine</country>
<wicri:noRegion>Wuhan 430070</wicri:noRegion>
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<author><name sortKey="Xiuxin Deng" sort="Xiuxin Deng" uniqKey="Xiuxin Deng" last="Xiuxin Deng">XIUXIN DENG</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>National key Laboratory of Crop Genetic Improvement, Huazhon Agricultural University</s1>
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<term>Hybrid</term>
<term>Production</term>
<term>Protoplast</term>
<term>Random amplified polymorphic DNA marker</term>
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<term>Protoplaste</term>
<term>Microcitrus papuana</term>
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<front><div type="abstract" xml:lang="en">X-ray irradiated embryogenic protoplasts of Microcitrus papuana Swing, were electrically fused with iodoacetic acid-treated embryogenic protoplasts of Newhall navel orange [Citrus sinensis (L.) Osb.]. Seven cell lines were established by low-melting agarose embedding culture of fusion-treated protoplasts. Cytological examination of 4 cell lines showed that each cell line consisted of many aneuploid (45.10%, 38.98%, 32.69% and 34.85%, respectively) and diploid cells (52.94%, 59.33%, 63.46% and 62.12%, respectively), whereas only a few tetraploid cells (1.96%, 1.69%, 3.85% and 3.03%, respectively) were detected. Analyses of random amplified polymorphic DNA with four 10-mer primers confirmed the hybrid characteristics of the cell lines, which in combination with chromosome counting proved that the cell lines were asymmetric hybrids.</div>
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<fC01 i1="01" l="ENG"><s0>X-ray irradiated embryogenic protoplasts of Microcitrus papuana Swing, were electrically fused with iodoacetic acid-treated embryogenic protoplasts of Newhall navel orange [Citrus sinensis (L.) Osb.]. Seven cell lines were established by low-melting agarose embedding culture of fusion-treated protoplasts. Cytological examination of 4 cell lines showed that each cell line consisted of many aneuploid (45.10%, 38.98%, 32.69% and 34.85%, respectively) and diploid cells (52.94%, 59.33%, 63.46% and 62.12%, respectively), whereas only a few tetraploid cells (1.96%, 1.69%, 3.85% and 3.03%, respectively) were detected. Analyses of random amplified polymorphic DNA with four 10-mer primers confirmed the hybrid characteristics of the cell lines, which in combination with chromosome counting proved that the cell lines were asymmetric hybrids.</s0>
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<s5>35</s5>
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<s5>53</s5>
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<s2>NS</s2>
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</record>

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Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis

Production of hybrid calluses via donor-recipient fusion between Microcitrus papuana and Citrus sinensis

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri