39K NMR measurement of intracellular potassium during ischemia in the perfused guinea pig heart.
Identifieur interne : 000744 ( PubMed/Corpus ); précédent : 000743; suivant : 00074539K NMR measurement of intracellular potassium during ischemia in the perfused guinea pig heart.
Auteurs : N B Radford ; E E Babcock ; A. Richman ; L. Szczepaniak ; C R Malloy ; A D SherrySource :
- Magnetic resonance in medicine [ 0740-3194 ] ; 1998.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Potassium.
- chemical : Organometallic Compounds, Organophosphorus Compounds, Thulium.
- metabolism : Myocardial Ischemia, Myocardium.
- methods : Magnetic Resonance Spectroscopy.
- Animals, Guinea Pigs, Perfusion, Spectrophotometry, Atomic.
Abstract
The hyperfine shift reagent, TmDOTP5-, was used to resolve the 39K NMR resonances of intra- (Ki+) and extracellular (Ke+) potassium in isolated, perfused guinea pig hearts. [Ki+] as measured by 39K NMR was 25.9 +/- 10.3 mM, compared with 114.4 +/- 10.8 mM as measured by atomic absorption spectroscopy (AAS) using TmDOTP5- as a marker of extracellular space. Thus, only approximately 23% of intracellular potassium was detected by 39K NMR using our experimental conditions. The area of the Ki+ signal increased during early ischemia then returned to baseline levels during reperfusion. In an effort to learn more about the Ki+ not detected by 39K NMR, hearts were perfused with a Rb+-enriched, K+-depleted buffer for an extended period. This resulted in loss of the entire 39K NMR signal, and Ki+, as measured by AAS, decreased from approximately 60 to approximately 6 to 7 micromol/g wet weight. When K+-depleted hearts were subjected to global ischemia, a small 39K NMR signal reappeared, suggesting that at least a portion of the nonexchangeable Ki+ becomes detectable by NMR during ischemia. This newly visible K+ signal subsequently dissipated during reperfusion of ischemic hearts. We conclude that ischemia induces changes in the NMR visibility of 39K in perfused guinea pig hearts.
PubMed: 9771571
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pubmed:9771571Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">39K NMR measurement of intracellular potassium during ischemia in the perfused guinea pig heart.</title><author><name sortKey="Radford, N B" sort="Radford, N B" uniqKey="Radford N" first="N B" last="Radford">N B Radford</name><affiliation><nlm:affiliation>University of Texas Southwestern Medical Center, Dallas, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Babcock, E E" sort="Babcock, E E" uniqKey="Babcock E" first="E E" last="Babcock">E E Babcock</name></author><author><name sortKey="Richman, A" sort="Richman, A" uniqKey="Richman A" first="A" last="Richman">A. Richman</name></author><author><name sortKey="Szczepaniak, L" sort="Szczepaniak, L" uniqKey="Szczepaniak L" first="L" last="Szczepaniak">L. Szczepaniak</name></author><author><name sortKey="Malloy, C R" sort="Malloy, C R" uniqKey="Malloy C" first="C R" last="Malloy">C R Malloy</name></author><author><name sortKey="Sherry, A D" sort="Sherry, A D" uniqKey="Sherry A" first="A D" last="Sherry">A D Sherry</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:9771571</idno><idno type="pmid">9771571</idno><idno type="wicri:Area/PubMed/Corpus">000744</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000744</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">39K NMR measurement of intracellular potassium during ischemia in the perfused guinea pig heart.</title><author><name sortKey="Radford, N B" sort="Radford, N B" uniqKey="Radford N" first="N B" last="Radford">N B Radford</name><affiliation><nlm:affiliation>University of Texas Southwestern Medical Center, Dallas, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Babcock, E E" sort="Babcock, E E" uniqKey="Babcock E" first="E E" last="Babcock">E E Babcock</name></author><author><name sortKey="Richman, A" sort="Richman, A" uniqKey="Richman A" first="A" last="Richman">A. Richman</name></author><author><name sortKey="Szczepaniak, L" sort="Szczepaniak, L" uniqKey="Szczepaniak L" first="L" last="Szczepaniak">L. Szczepaniak</name></author><author><name sortKey="Malloy, C R" sort="Malloy, C R" uniqKey="Malloy C" first="C R" last="Malloy">C R Malloy</name></author><author><name sortKey="Sherry, A D" sort="Sherry, A D" uniqKey="Sherry A" first="A D" last="Sherry">A D Sherry</name></author></analytic><series><title level="j">Magnetic resonance in medicine</title><idno type="ISSN">0740-3194</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Guinea Pigs</term><term>Magnetic Resonance Spectroscopy (methods)</term><term>Myocardial Ischemia (metabolism)</term><term>Myocardium (metabolism)</term><term>Organometallic Compounds</term><term>Organophosphorus Compounds</term><term>Perfusion</term><term>Potassium (metabolism)</term><term>Spectrophotometry, Atomic</term><term>Thulium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Potassium</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Organometallic Compounds</term><term>Organophosphorus Compounds</term><term>Thulium</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Myocardial Ischemia</term><term>Myocardium</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Magnetic Resonance Spectroscopy</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Guinea Pigs</term><term>Perfusion</term><term>Spectrophotometry, Atomic</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The hyperfine shift reagent, TmDOTP5-, was used to resolve the 39K NMR resonances of intra- (Ki+) and extracellular (Ke+) potassium in isolated, perfused guinea pig hearts. [Ki+] as measured by 39K NMR was 25.9 +/- 10.3 mM, compared with 114.4 +/- 10.8 mM as measured by atomic absorption spectroscopy (AAS) using TmDOTP5- as a marker of extracellular space. Thus, only approximately 23% of intracellular potassium was detected by 39K NMR using our experimental conditions. The area of the Ki+ signal increased during early ischemia then returned to baseline levels during reperfusion. In an effort to learn more about the Ki+ not detected by 39K NMR, hearts were perfused with a Rb+-enriched, K+-depleted buffer for an extended period. This resulted in loss of the entire 39K NMR signal, and Ki+, as measured by AAS, decreased from approximately 60 to approximately 6 to 7 micromol/g wet weight. When K+-depleted hearts were subjected to global ischemia, a small 39K NMR signal reappeared, suggesting that at least a portion of the nonexchangeable Ki+ becomes detectable by NMR during ischemia. This newly visible K+ signal subsequently dissipated during reperfusion of ischemic hearts. We conclude that ischemia induces changes in the NMR visibility of 39K in perfused guinea pig hearts.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">9771571</PMID><DateCreated><Year>1998</Year><Month>12</Month><Day>21</Day></DateCreated><DateCompleted><Year>1998</Year><Month>12</Month><Day>21</Day></DateCompleted><DateRevised><Year>2015</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0740-3194</ISSN><JournalIssue CitedMedium="Print"><Volume>40</Volume><Issue>4</Issue><PubDate><Year>1998</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Magnetic resonance in medicine</Title><ISOAbbreviation>Magn Reson Med</ISOAbbreviation></Journal><ArticleTitle>39K NMR measurement of intracellular potassium during ischemia in the perfused guinea pig heart.</ArticleTitle><Pagination><MedlinePgn>544-50</MedlinePgn></Pagination><Abstract><AbstractText>The hyperfine shift reagent, TmDOTP5-, was used to resolve the 39K NMR resonances of intra- (Ki+) and extracellular (Ke+) potassium in isolated, perfused guinea pig hearts. [Ki+] as measured by 39K NMR was 25.9 +/- 10.3 mM, compared with 114.4 +/- 10.8 mM as measured by atomic absorption spectroscopy (AAS) using TmDOTP5- as a marker of extracellular space. Thus, only approximately 23% of intracellular potassium was detected by 39K NMR using our experimental conditions. The area of the Ki+ signal increased during early ischemia then returned to baseline levels during reperfusion. In an effort to learn more about the Ki+ not detected by 39K NMR, hearts were perfused with a Rb+-enriched, K+-depleted buffer for an extended period. This resulted in loss of the entire 39K NMR signal, and Ki+, as measured by AAS, decreased from approximately 60 to approximately 6 to 7 micromol/g wet weight. When K+-depleted hearts were subjected to global ischemia, a small 39K NMR signal reappeared, suggesting that at least a portion of the nonexchangeable Ki+ becomes detectable by NMR during ischemia. This newly visible K+ signal subsequently dissipated during reperfusion of ischemic hearts. We conclude that ischemia induces changes in the NMR visibility of 39K in perfused guinea pig hearts.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Radford</LastName><ForeName>N B</ForeName><Initials>NB</Initials><AffiliationInfo><Affiliation>University of Texas Southwestern Medical Center, Dallas, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Babcock</LastName><ForeName>E E</ForeName><Initials>EE</Initials></Author><Author ValidYN="Y"><LastName>Richman</LastName><ForeName>A</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Szczepaniak</LastName><ForeName>L</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Malloy</LastName><ForeName>C R</ForeName><Initials>CR</Initials></Author><Author ValidYN="Y"><LastName>Sherry</LastName><ForeName>A D</ForeName><Initials>AD</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>P41-RR02584</GrantID><Acronym>RR</Acronym><Agency>NCRR NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>UNITED STATES</Country><MedlineTA>Magn Reson Med</MedlineTA><NlmUniqueID>8505245</NlmUniqueID><ISSNLinking>0740-3194</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009942">Organometallic Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009943">Organophosphorus Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C069929">thulium(III) 1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetramethylenephosphonate</NameOfSubstance></Chemical><Chemical><RegistryNumber>8RKC5ATI4P</RegistryNumber><NameOfSubstance UI="D013932">Thulium</NameOfSubstance></Chemical><Chemical><RegistryNumber>RWP5GA015D</RegistryNumber><NameOfSubstance UI="D011188">Potassium</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D000818">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006168">Guinea Pigs</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D009682">Magnetic Resonance Spectroscopy</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000379">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017202">Myocardial Ischemia</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D009206">Myocardium</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D009942">Organometallic Compounds</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D009943">Organophosphorus Compounds</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D010477">Perfusion</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D011188">Potassium</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013054">Spectrophotometry, Atomic</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013932">Thulium</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>10</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1998</Year><Month>10</Month><Day>15</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1998</Year><Month>10</Month><Day>15</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9771571</ArticleId></ArticleIdList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix (Dilib)
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