7Li nuclear magnetic resonance study for the determination of Li+ properties in neuroblastoma SH-SY5Y cells.
Identifieur interne : 000745 ( PubMed/Corpus ); précédent : 000744; suivant : 0007467Li nuclear magnetic resonance study for the determination of Li+ properties in neuroblastoma SH-SY5Y cells.
Auteurs : J. Nikolakopoulos ; C. Zachariah ; D. Mota De Freitas ; E B Stubbs ; R. Ramasamy ; M C Castro ; C F GeraldesSource :
- Journal of neurochemistry [ 0022-3042 ] ; 1998.
English descriptors
- KwdEn :
- 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid (pharmacology), Cell Survival (drug effects), Humans, Intracellular Fluid (chemistry), Intracellular Fluid (metabolism), Ion Transport (drug effects), Isotopes, Lithium (chemistry), Lithium (metabolism), Lithium (pharmacology), Magnetic Resonance Spectroscopy, Neuroblastoma (chemistry), Neuroblastoma (metabolism), Neuroblastoma (pathology), Ouabain (pharmacology), Perfusion, Phloretin (pharmacology), Tumor Cells, Cultured, Veratridine (pharmacology).
- MESH :
- chemical , chemistry : Lithium.
- chemical , metabolism : Lithium.
- chemical , pharmacology : 4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid, Lithium, Ouabain, Phloretin, Veratridine.
- chemistry : Intracellular Fluid, Neuroblastoma.
- drug effects : Cell Survival, Ion Transport.
- metabolism : Intracellular Fluid, Neuroblastoma.
- pathology : Neuroblastoma.
- Humans, Isotopes, Magnetic Resonance Spectroscopy, Perfusion, Tumor Cells, Cultured.
Abstract
Lithium has been used clinically in the treatment of manic depression. However, its pharmacologic mode of action remains unclear. Characteristics of Li+ interactions in red blood cells (RBCs) have been identified. We investigated Li+ interactions on human neuroblastoma SH-SY5Y cells by developing a novel 7Li NMR method that provided a clear estimation of the intra- and extracellular amounts of Li+ in the presence of the shift reagent thulium-1,4,7,10-tetrazacyclododecane-N,N',N'',N'''-tetramethylene phosphonate (HTmDOTP4-). The first-order rate constants of Li+ influx and efflux for perfused, agarose-embedded SH-SY5Y cells in the presence of 3 mM HTmDOTP4- were 0.055 +/- 0.006 (n = 4) and -0.025 +/- 0.006 min(-1) (n = 3), respectively. Significant increases in the rate constants of Li+ influx and efflux in the presence of 0.05 mM veratridine indicated the presence of Na+ channel-mediated Li+ transport in SH-SY5Y cells. 7Li NMR relaxation measurements showed that Li+ is immobilized more in human neuroblastoma SH-SY5Y cells than in human RBCs.
PubMed: 9751202
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">7Li nuclear magnetic resonance study for the determination of Li+ properties in neuroblastoma SH-SY5Y cells.</title><author><name sortKey="Nikolakopoulos, J" sort="Nikolakopoulos, J" uniqKey="Nikolakopoulos J" first="J" last="Nikolakopoulos">J. Nikolakopoulos</name><affiliation><nlm:affiliation>Department of Chemistry, Loyola University Chicago, Illinois 60626, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zachariah, C" sort="Zachariah, C" uniqKey="Zachariah C" first="C" last="Zachariah">C. Zachariah</name></author><author><name sortKey="Mota De Freitas, D" sort="Mota De Freitas, D" uniqKey="Mota De Freitas D" first="D" last="Mota De Freitas">D. Mota De Freitas</name></author><author><name sortKey="Stubbs, E B" sort="Stubbs, E B" uniqKey="Stubbs E" first="E B" last="Stubbs">E B Stubbs</name></author><author><name sortKey="Ramasamy, R" sort="Ramasamy, R" uniqKey="Ramasamy R" first="R" last="Ramasamy">R. Ramasamy</name></author><author><name sortKey="Castro, M C" sort="Castro, M C" uniqKey="Castro M" first="M C" last="Castro">M C Castro</name></author><author><name sortKey="Geraldes, C F" sort="Geraldes, C F" uniqKey="Geraldes C" first="C F" last="Geraldes">C F Geraldes</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1998">1998</date><idno type="RBID">pubmed:9751202</idno><idno type="pmid">9751202</idno><idno type="wicri:Area/PubMed/Corpus">000745</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000745</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">7Li nuclear magnetic resonance study for the determination of Li+ properties in neuroblastoma SH-SY5Y cells.</title><author><name sortKey="Nikolakopoulos, J" sort="Nikolakopoulos, J" uniqKey="Nikolakopoulos J" first="J" last="Nikolakopoulos">J. Nikolakopoulos</name><affiliation><nlm:affiliation>Department of Chemistry, Loyola University Chicago, Illinois 60626, USA.</nlm:affiliation></affiliation></author><author><name sortKey="Zachariah, C" sort="Zachariah, C" uniqKey="Zachariah C" first="C" last="Zachariah">C. Zachariah</name></author><author><name sortKey="Mota De Freitas, D" sort="Mota De Freitas, D" uniqKey="Mota De Freitas D" first="D" last="Mota De Freitas">D. Mota De Freitas</name></author><author><name sortKey="Stubbs, E B" sort="Stubbs, E B" uniqKey="Stubbs E" first="E B" last="Stubbs">E B Stubbs</name></author><author><name sortKey="Ramasamy, R" sort="Ramasamy, R" uniqKey="Ramasamy R" first="R" last="Ramasamy">R. Ramasamy</name></author><author><name sortKey="Castro, M C" sort="Castro, M C" uniqKey="Castro M" first="M C" last="Castro">M C Castro</name></author><author><name sortKey="Geraldes, C F" sort="Geraldes, C F" uniqKey="Geraldes C" first="C F" last="Geraldes">C F Geraldes</name></author></analytic><series><title level="j">Journal of neurochemistry</title><idno type="ISSN">0022-3042</idno><imprint><date when="1998" type="published">1998</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid (pharmacology)</term><term>Cell Survival (drug effects)</term><term>Humans</term><term>Intracellular Fluid (chemistry)</term><term>Intracellular Fluid (metabolism)</term><term>Ion Transport (drug effects)</term><term>Isotopes</term><term>Lithium (chemistry)</term><term>Lithium (metabolism)</term><term>Lithium (pharmacology)</term><term>Magnetic Resonance Spectroscopy</term><term>Neuroblastoma (chemistry)</term><term>Neuroblastoma (metabolism)</term><term>Neuroblastoma (pathology)</term><term>Ouabain (pharmacology)</term><term>Perfusion</term><term>Phloretin (pharmacology)</term><term>Tumor Cells, Cultured</term><term>Veratridine (pharmacology)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Lithium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Lithium</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid</term><term>Lithium</term><term>Ouabain</term><term>Phloretin</term><term>Veratridine</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Intracellular Fluid</term><term>Neuroblastoma</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Survival</term><term>Ion Transport</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Intracellular Fluid</term><term>Neuroblastoma</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Neuroblastoma</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Isotopes</term><term>Magnetic Resonance Spectroscopy</term><term>Perfusion</term><term>Tumor Cells, Cultured</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lithium has been used clinically in the treatment of manic depression. However, its pharmacologic mode of action remains unclear. Characteristics of Li+ interactions in red blood cells (RBCs) have been identified. We investigated Li+ interactions on human neuroblastoma SH-SY5Y cells by developing a novel 7Li NMR method that provided a clear estimation of the intra- and extracellular amounts of Li+ in the presence of the shift reagent thulium-1,4,7,10-tetrazacyclododecane-N,N',N'',N'''-tetramethylene phosphonate (HTmDOTP4-). The first-order rate constants of Li+ influx and efflux for perfused, agarose-embedded SH-SY5Y cells in the presence of 3 mM HTmDOTP4- were 0.055 +/- 0.006 (n = 4) and -0.025 +/- 0.006 min(-1) (n = 3), respectively. Significant increases in the rate constants of Li+ influx and efflux in the presence of 0.05 mM veratridine indicated the presence of Na+ channel-mediated Li+ transport in SH-SY5Y cells. 7Li NMR relaxation measurements showed that Li+ is immobilized more in human neuroblastoma SH-SY5Y cells than in human RBCs.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">9751202</PMID><DateCreated><Year>1998</Year><Month>10</Month><Day>14</Day></DateCreated><DateCompleted><Year>1998</Year><Month>10</Month><Day>14</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-3042</ISSN><JournalIssue CitedMedium="Print"><Volume>71</Volume><Issue>4</Issue><PubDate><Year>1998</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of neurochemistry</Title><ISOAbbreviation>J. Neurochem.</ISOAbbreviation></Journal><ArticleTitle>7Li nuclear magnetic resonance study for the determination of Li+ properties in neuroblastoma SH-SY5Y cells.</ArticleTitle><Pagination><MedlinePgn>1676-84</MedlinePgn></Pagination><Abstract><AbstractText>Lithium has been used clinically in the treatment of manic depression. However, its pharmacologic mode of action remains unclear. Characteristics of Li+ interactions in red blood cells (RBCs) have been identified. We investigated Li+ interactions on human neuroblastoma SH-SY5Y cells by developing a novel 7Li NMR method that provided a clear estimation of the intra- and extracellular amounts of Li+ in the presence of the shift reagent thulium-1,4,7,10-tetrazacyclododecane-N,N',N'',N'''-tetramethylene phosphonate (HTmDOTP4-). The first-order rate constants of Li+ influx and efflux for perfused, agarose-embedded SH-SY5Y cells in the presence of 3 mM HTmDOTP4- were 0.055 +/- 0.006 (n = 4) and -0.025 +/- 0.006 min(-1) (n = 3), respectively. Significant increases in the rate constants of Li+ influx and efflux in the presence of 0.05 mM veratridine indicated the presence of Na+ channel-mediated Li+ transport in SH-SY5Y cells. 7Li NMR relaxation measurements showed that Li+ is immobilized more in human neuroblastoma SH-SY5Y cells than in human RBCs.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Nikolakopoulos</LastName><ForeName>J</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Loyola University Chicago, Illinois 60626, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zachariah</LastName><ForeName>C</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Mota de Freitas</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Stubbs</LastName><ForeName>E B</ForeName><Initials>EB</Initials><Suffix>Jr</Suffix></Author><Author ValidYN="Y"><LastName>Ramasamy</LastName><ForeName>R</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Castro</LastName><ForeName>M C</ForeName><Initials>MC</Initials></Author><Author ValidYN="Y"><LastName>Geraldes</LastName><ForeName>C F</ForeName><Initials>CF</Initials></Author></AuthorList><Language>eng</Language><GrantList CompleteYN="Y"><Grant><GrantID>MH45926</GrantID><Acronym>MH</Acronym><Agency>NIMH NIH HHS</Agency><Country>United States</Country></Grant></GrantList><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType><PublicationType UI="D013487">Research Support, U.S. Gov't, P.H.S.</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>UNITED STATES</Country><MedlineTA>J Neurochem</MedlineTA><NlmUniqueID>2985190R</NlmUniqueID><ISSNLinking>0022-3042</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007554">Isotopes</NameOfSubstance></Chemical><Chemical><RegistryNumber>5ACL011P69</RegistryNumber><NameOfSubstance UI="D010042">Ouabain</NameOfSubstance></Chemical><Chemical><RegistryNumber>71-62-5</RegistryNumber><NameOfSubstance UI="D014701">Veratridine</NameOfSubstance></Chemical><Chemical><RegistryNumber>9FN79X2M3F</RegistryNumber><NameOfSubstance UI="D008094">Lithium</NameOfSubstance></Chemical><Chemical><RegistryNumber>Q1O6DSW23R</RegistryNumber><NameOfSubstance UI="D017878">4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>S5J5OE47MK</RegistryNumber><NameOfSubstance UI="D010693">Phloretin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017878">4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000494">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D002470">Cell Survival</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000187">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D006801">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007424">Intracellular Fluid</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="N" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D017136">Ion Transport</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000187">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D007554">Isotopes</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008094">Lithium</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName><QualifierName MajorTopicYN="N" UI="Q000494">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D009682">Magnetic Resonance Spectroscopy</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D009447">Neuroblastoma</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000737">chemistry</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName><QualifierName MajorTopicYN="N" UI="Q000473">pathology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D010042">Ouabain</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000494">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D010477">Perfusion</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D010693">Phloretin</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000494">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D014407">Tumor Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D014701">Veratridine</DescriptorName><QualifierName MajorTopicYN="N" UI="Q000494">pharmacology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1998</Year><Month>9</Month><Day>29</Day><Hour>4</Hour><Minute>4</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2001</Year><Month>3</Month><Day>28</Day><Hour>10</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate 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