In vivo 3D molecular imaging with BIRDS at high spatiotemporal resolution
Identifieur interne : 000713 ( Ncbi/Merge ); précédent : 000712; suivant : 000714In vivo 3D molecular imaging with BIRDS at high spatiotemporal resolution
Auteurs : Daniel Coman [États-Unis] ; Robin A. De Graaf [États-Unis] ; Douglas L. Rothman [États-Unis] ; Fahmeed Hyder [États-Unis]Source :
- NMR in biomedicine [ 0952-3480 ] ; 2013.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical : Organometallic Compounds.
- methods : Biosensing Techniques, Imaging, Three-Dimensional, Molecular Imaging.
- physiology : Brain.
- Animals, Body Temperature, Magnetic Resonance Imaging, Normal Distribution, Rats, Rats, Sprague-Dawley, Spatio-Temporal Analysis.
Abstract
Spectroscopic signals which emanate from complexes between paramagnetic lanthanide III ions (e.g., Tm3+) and macrocyclic chelates (e.g., 1,4,7,10-tetramethyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate, or DOTMA4−) are sensitive to physiology (e.g., temperature and/or pH). Because non-exchanging protons from these lanthanide-based macrocyclic agents have relaxation times on the order of a few milliseconds, rapid data acquisition is possible with chemical shift imaging (CSI). Thus
Url:
DOI: 10.1002/nbm.2995
PubMed: 23881869
PubMed Central: 3800475
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<term>Imagerie tridimensionnelle ()</term>
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<term>Techniques de biocapteur ()</term>
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<front><div type="abstract" xml:lang="en"><p id="P1">Spectroscopic signals which emanate from complexes between paramagnetic lanthanide III ions (e.g., Tm<sup>3+</sup>
) and macrocyclic chelates (e.g., 1,4,7,10-tetramethyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate, or DOTMA<sup>4−</sup>
) are sensitive to physiology (e.g., temperature and/or pH). Because non-exchanging protons from these lanthanide-based macrocyclic agents have relaxation times on the order of a few milliseconds, rapid data acquisition is possible with chemical shift imaging (CSI). Thus <italic><bold>B</bold>
</italic>
iosensor <italic><bold>I</bold>
</italic>
maging of <italic><bold>R</bold>
</italic>
edundant <italic><bold>D</bold>
</italic>
eviation in <italic><bold>S</bold>
</italic>
hifts (BIRDS) which originate from non-exchanging protons of these paramagnetic agents, but exclude water proton detection, can allow molecular imaging. Previous 2D CSI experiments with such lanthanide-based macrocyclics allowed acquisition from ~12 µL voxels in rat brain within 5 minutes using rectangular encoding of k-space. Because cubical encoding of k-space in 3D for whole brain coverage increases CSI acquisition time to several tens of minutes or more, a faster CSI technique is required for BIRDS to be of practical use. Here we demonstrate a CSI acquisition method to improve 3D molecular imaging capabilities with lanthanide-based macrocyclics. Using TmDOTMA<sup>−</sup>
, we show datasets from a 20×20×20 mm<sup>3</sup>
field-of-view with voxels of ~1 µL effective volume acquired within 5 minutes (at 11.7T) for temperature mapping. By employing <bold>re</bold>
duced <bold>s</bold>
pherical <bold>e</bold>
ncoding with <bold>Ga</bold>
ussian <bold>w</bold>
eighting (RESEGAW) instead of cubical encoding of k-space, a significant increase in CSI signal is obtained. <italic>In vitro</italic>
and <italic>in vivo</italic>
3D CSI data with TmDOTMA<sup>−</sup>
, and presumably similar lanthanide-based macrocyclics, suggest that acquisition using RESEGAW can be used for high spatiotemporal molecular mapping with BIRDS.</p>
</div>
</front>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">In vivo 3D molecular imaging with BIRDS at high spatiotemporal resolution</title>
<author><name sortKey="Coman, Daniel" sort="Coman, Daniel" uniqKey="Coman D" first="Daniel" last="Coman">Daniel Coman</name>
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<affiliation wicri:level="2"><nlm:aff id="A2">Core Center for Quantitative Neuroscience with Magnetic Resonance (QNMR), Yale University, New Haven, CT, USA;</nlm:aff>
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<affiliation wicri:level="2"><nlm:aff id="A3">Department of Diagnostic Radiology, Yale University, New Haven, CT, USA</nlm:aff>
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<author><name sortKey="De Graaf, Robin A" sort="De Graaf, Robin A" uniqKey="De Graaf R" first="Robin A." last="De Graaf">Robin A. De Graaf</name>
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<affiliation wicri:level="2"><nlm:aff id="A4">Department of Biomedical Engineering, Yale University, New Haven, CT, USA</nlm:aff>
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<author><name sortKey="Rothman, Douglas L" sort="Rothman, Douglas L" uniqKey="Rothman D" first="Douglas L." last="Rothman">Douglas L. Rothman</name>
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<placeName><region type="state">Connecticut</region>
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<affiliation wicri:level="2"><nlm:aff id="A3">Department of Diagnostic Radiology, Yale University, New Haven, CT, USA</nlm:aff>
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<affiliation wicri:level="2"><nlm:aff id="A4">Department of Biomedical Engineering, Yale University, New Haven, CT, USA</nlm:aff>
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<author><name sortKey="Hyder, Fahmeed" sort="Hyder, Fahmeed" uniqKey="Hyder F" first="Fahmeed" last="Hyder">Fahmeed Hyder</name>
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<front><div type="abstract" xml:lang="en"><p id="P1">Spectroscopic signals which emanate from complexes between paramagnetic lanthanide III ions (e.g., Tm<sup>3+</sup>
) and macrocyclic chelates (e.g., 1,4,7,10-tetramethyl 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate, or DOTMA<sup>4−</sup>
) are sensitive to physiology (e.g., temperature and/or pH). Because non-exchanging protons from these lanthanide-based macrocyclic agents have relaxation times on the order of a few milliseconds, rapid data acquisition is possible with chemical shift imaging (CSI). Thus <italic><bold>B</bold>
</italic>
iosensor <italic><bold>I</bold>
</italic>
maging of <italic><bold>R</bold>
</italic>
edundant <italic><bold>D</bold>
</italic>
eviation in <italic><bold>S</bold>
</italic>
hifts (BIRDS) which originate from non-exchanging protons of these paramagnetic agents, but exclude water proton detection, can allow molecular imaging. Previous 2D CSI experiments with such lanthanide-based macrocyclics allowed acquisition from ~12 µL voxels in rat brain within 5 minutes using rectangular encoding of k-space. Because cubical encoding of k-space in 3D for whole brain coverage increases CSI acquisition time to several tens of minutes or more, a faster CSI technique is required for BIRDS to be of practical use. Here we demonstrate a CSI acquisition method to improve 3D molecular imaging capabilities with lanthanide-based macrocyclics. Using TmDOTMA<sup>−</sup>
, we show datasets from a 20×20×20 mm<sup>3</sup>
field-of-view with voxels of ~1 µL effective volume acquired within 5 minutes (at 11.7T) for temperature mapping. By employing <bold>re</bold>
duced <bold>s</bold>
pherical <bold>e</bold>
ncoding with <bold>Ga</bold>
ussian <bold>w</bold>
eighting (RESEGAW) instead of cubical encoding of k-space, a significant increase in CSI signal is obtained. <italic>In vitro</italic>
and <italic>in vivo</italic>
3D CSI data with TmDOTMA<sup>−</sup>
, and presumably similar lanthanide-based macrocyclics, suggest that acquisition using RESEGAW can be used for high spatiotemporal molecular mapping with BIRDS.</p>
</div>
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<author><name sortKey="Hyder, Fahmeed" sort="Hyder, Fahmeed" uniqKey="Hyder F" first="Fahmeed" last="Hyder">Fahmeed Hyder</name>
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<term>Biosensing Techniques (methods)</term>
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<term>Imaging, Three-Dimensional (methods)</term>
<term>Magnetic Resonance Imaging</term>
<term>Molecular Imaging (methods)</term>
<term>Normal Distribution</term>
<term>Organometallic Compounds</term>
<term>Rats</term>
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<term>Imagerie moléculaire ()</term>
<term>Imagerie par résonance magnétique</term>
<term>Imagerie tridimensionnelle ()</term>
<term>Loi normale</term>
<term>Rat Sprague-Dawley</term>
<term>Rats</term>
<term>Techniques de biocapteur ()</term>
<term>Température du corps</term>
</keywords>
<keywords scheme="MESH" type="chemical" xml:lang="en"><term>Organometallic Compounds</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term>
<term>Imaging, Three-Dimensional</term>
<term>Molecular Imaging</term>
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<term>Magnetic Resonance Imaging</term>
<term>Normal Distribution</term>
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<term>Spatio-Temporal Analysis</term>
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<term>Imagerie moléculaire</term>
<term>Imagerie par résonance magnétique</term>
<term>Imagerie tridimensionnelle</term>
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<front><div type="abstract" xml:lang="en">Spectroscopic signals which emanate from complexes between paramagnetic lanthanide (III) ions (e.g. Tm(3+)) and macrocyclic chelates (e.g. 1,4,7,10-tetramethyl-1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetate, or DOTMA(4-)) are sensitive to physiology (e.g. temperature). Because nonexchanging protons from these lanthanide-based macrocyclic agents have relaxation times on the order of a few milliseconds, rapid data acquisition is possible with chemical shift imaging (CSI). Thus, Biosensor Imaging of Redundant Deviation in Shifts (BIRDS) which originate from nonexchanging protons of these paramagnetic agents, but exclude water proton detection, can allow molecular imaging. Previous two-dimensional CSI experiments with such lanthanide-based macrocyclics allowed acquisition from ~12-μL voxels in rat brain within 5 min using rectangular encoding of k space. Because cubical encoding of k space in three dimensions for whole-brain coverage increases the CSI acquisition time to several tens of minutes or more, a faster CSI technique is required for BIRDS to be of practical use. Here, we demonstrate a CSI acquisition method to improve three-dimensional molecular imaging capabilities with lanthanide-based macrocyclics. Using TmDOTMA(-), we show datasets from a 20 × 20 × 20-mm(3) field of view with voxels of ~1 μL effective volume acquired within 5 min (at 11.7 T) for temperature mapping. By employing reduced spherical encoding with Gaussian weighting (RESEGAW) instead of cubical encoding of k space, a significant increase in CSI signal is obtained. In vitro and in vivo three-dimensional CSI data with TmDOTMA(-), and presumably similar lanthanide-based macrocyclics, suggest that acquisition using RESEGAW can be used for high spatiotemporal resolution molecular mapping with BIRDS.</div>
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