Differential anodic oxidation of single organic linkage molecules enabling orthogonal bio-immobilization
Identifieur interne : 000F36 ( Main/Repository ); précédent : 000F35; suivant : 000F37Differential anodic oxidation of single organic linkage molecules enabling orthogonal bio-immobilization
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Abstract
We report a novel method for preparing electrode surfaces that present thiol-containing or thiol-reactive groups using a single organic silane linkage terminated with 1,3-dithiane via differential anodic oxidation of 1,3-dithiane. We found that the surface groups resulting from the anodic oxidation reaction differed according to the concentration of H2O in the electrolyte solution during the oxidation reaction. The differential anodic oxidation was used successfully for the orthogonal immobilization of two different proteins. We demonstrated also the preparation of an immunoassay platform via orthogonal immobilization, which was then used to detect multiple target antigens based on a sandwich immunoassay.
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">Differential anodic oxidation of single organic linkage molecules enabling orthogonal bio-immobilization</title><author><name sortKey="Kwon, Seung Ryong" uniqKey="Kwon S">Seung-Ryong Kwon</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, University of Incheon</s1><s2>Incheon, 406-772</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Corée du Sud</country><wicri:noRegion>Incheon, 406-772</wicri:noRegion></affiliation></author><author><name sortKey="Ko, Hyunjun" uniqKey="Ko H">Hyunjun Ko</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, University of Incheon</s1><s2>Incheon, 406-772</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Corée du Sud</country><wicri:noRegion>Incheon, 406-772</wicri:noRegion></affiliation></author><author><name>HYEON JIN KIM</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, University of Incheon</s1><s2>Incheon, 406-772</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Corée du Sud</country><wicri:noRegion>Incheon, 406-772</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Kyuwon" uniqKey="Kim K">Kyuwon Kim</name><affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Department of Chemistry, University of Incheon</s1><s2>Incheon, 406-772</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></inist:fA14><country>Corée du Sud</country><wicri:noRegion>Incheon, 406-772</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="inist">13-0192346</idno><date when="2013">2013</date><idno type="stanalyst">PASCAL 13-0192346 INIST</idno><idno type="RBID">Pascal:13-0192346</idno><idno type="wicri:Area/Main/Corpus">000D34</idno><idno type="wicri:Area/Main/Repository">000F36</idno></publicationStmt><seriesStmt><idno type="ISSN">1388-2481</idno><title level="j" type="abbreviated">Electrochem. commun.</title><title level="j" type="main">Electrochemistry communications</title></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Anodizing</term><term>Antibody</term><term>Antigen</term><term>Functionalization</term><term>Immobilization</term><term>Immunosensor</term><term>Indium tin oxide electrode</term><term>Microelectrode</term><term>Optically transparent electrode</term><term>Organic siloxane</term><term>Self assembly</term><term>Thiol</term></keywords><keywords scheme="Pascal" xml:lang="fr"><term>Anodisation</term><term>Immobilisation</term><term>Siloxane organique</term><term>Thiol</term><term>Autoassemblage</term><term>Immunodétecteur</term><term>Electrode ITO</term><term>Fonctionnalisation</term><term>Anticorps</term><term>Antigène</term><term>Microélectrode</term><term>Electrode optiquement transparente</term><term>1,3-Dithiane-4-valéramide dérivé</term><term>1,2-Dithiolane-1,1-dioxyde dérivé</term><term>1,2-Dithiolane dérivé</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We report a novel method for preparing electrode surfaces that present thiol-containing or thiol-reactive groups using a single organic silane linkage terminated with 1,3-dithiane via differential anodic oxidation of 1,3-dithiane. We found that the surface groups resulting from the anodic oxidation reaction differed according to the concentration of H<sub>2</sub>O in the electrolyte solution during the oxidation reaction. The differential anodic oxidation was used successfully for the orthogonal immobilization of two different proteins. We demonstrated also the preparation of an immunoassay platform via orthogonal immobilization, which was then used to detect multiple target antigens based on a sandwich immunoassay.</div></front></TEI><inist><standard h6="B"><pA><fA01 i1="01" i2="1"><s0>1388-2481</s0></fA01><fA03 i2="1"><s0>Electrochem. commun.</s0></fA03><fA05><s2>31</s2></fA05><fA08 i1="01" i2="1" l="ENG"><s1>Differential anodic oxidation of single organic linkage molecules enabling orthogonal bio-immobilization</s1></fA08><fA11 i1="01" i2="1"><s1>KWON (Seung-Ryong)</s1></fA11><fA11 i1="02" i2="1"><s1>KO (Hyunjun)</s1></fA11><fA11 i1="03" i2="1"><s1>HYEON JIN KIM</s1></fA11><fA11 i1="04" i2="1"><s1>KIM (Kyuwon)</s1></fA11><fA14 i1="01"><s1>Department of Chemistry, University of Incheon</s1><s2>Incheon, 406-772</s2><s3>KOR</s3><sZ>1 aut.</sZ><sZ>2 aut.</sZ><sZ>3 aut.</sZ><sZ>4 aut.</sZ></fA14><fA20><s1>96-99</s1></fA20><fA21><s1>2013</s1></fA21><fA23 i1="01"><s0>ENG</s0></fA23><fA43 i1="01"><s1>INIST</s1><s2>26863</s2><s5>354000504113890260</s5></fA43><fA44><s0>0000</s0><s1>© 2013 INIST-CNRS. All rights reserved.</s1></fA44><fA45><s0>10 ref.</s0></fA45><fA47 i1="01" i2="1"><s0>13-0192346</s0></fA47><fA60><s1>P</s1></fA60><fA61><s0>A</s0></fA61><fA64 i1="01" i2="1"><s0>Electrochemistry communications</s0></fA64><fA66 i1="01"><s0>NLD</s0></fA66><fC01 i1="01" l="ENG"><s0>We report a novel method for preparing electrode surfaces that present thiol-containing or thiol-reactive groups using a single organic silane linkage terminated with 1,3-dithiane via differential anodic oxidation of 1,3-dithiane. We found that the surface groups resulting from the anodic oxidation reaction differed according to the concentration of H<sub>2</sub>O in the electrolyte solution during the oxidation reaction. The differential anodic oxidation was used successfully for the orthogonal immobilization of two different proteins. We demonstrated also the preparation of an immunoassay platform via orthogonal immobilization, which was then used to detect multiple target antigens based on a sandwich immunoassay.</s0></fC01><fC02 i1="01" i2="X"><s0>002A31C09B</s0></fC02><fC02 i1="02" i2="X"><s0>001C01H02B</s0></fC02><fC02 i1="03" i2="X"><s0>215</s0></fC02><fC03 i1="01" i2="X" l="FRE"><s0>Anodisation</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="ENG"><s0>Anodizing</s0><s5>01</s5></fC03><fC03 i1="01" i2="X" l="SPA"><s0>Anodización</s0><s5>01</s5></fC03><fC03 i1="02" i2="X" l="FRE"><s0>Immobilisation</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="ENG"><s0>Immobilization</s0><s5>02</s5></fC03><fC03 i1="02" i2="X" l="SPA"><s0>Inmovilización</s0><s5>02</s5></fC03><fC03 i1="03" i2="X" l="FRE"><s0>Siloxane organique</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="ENG"><s0>Organic siloxane</s0><s5>03</s5></fC03><fC03 i1="03" i2="X" l="SPA"><s0>Siloxano orgánico</s0><s5>03</s5></fC03><fC03 i1="04" i2="X" l="FRE"><s0>Thiol</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="ENG"><s0>Thiol</s0><s5>04</s5></fC03><fC03 i1="04" i2="X" l="SPA"><s0>Tiol</s0><s5>04</s5></fC03><fC03 i1="05" i2="X" l="FRE"><s0>Autoassemblage</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="ENG"><s0>Self assembly</s0><s5>05</s5></fC03><fC03 i1="05" i2="X" l="SPA"><s0>Autoensamble</s0><s5>05</s5></fC03><fC03 i1="06" i2="X" l="FRE"><s0>Immunodétecteur</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="ENG"><s0>Immunosensor</s0><s5>06</s5></fC03><fC03 i1="06" i2="X" l="SPA"><s0>Inmunodetector</s0><s5>06</s5></fC03><fC03 i1="07" i2="X" l="FRE"><s0>Electrode ITO</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="ENG"><s0>Indium tin oxide electrode</s0><s5>07</s5></fC03><fC03 i1="07" i2="X" l="SPA"><s0>Electrodo ITO</s0><s5>07</s5></fC03><fC03 i1="08" i2="X" l="FRE"><s0>Fonctionnalisation</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="ENG"><s0>Functionalization</s0><s5>08</s5></fC03><fC03 i1="08" i2="X" l="SPA"><s0>Funciónalización</s0><s5>08</s5></fC03><fC03 i1="09" i2="X" l="FRE"><s0>Anticorps</s0><s5>10</s5></fC03><fC03 i1="09" i2="X" l="ENG"><s0>Antibody</s0><s5>10</s5></fC03><fC03 i1="09" i2="X" l="SPA"><s0>Anticuerpo</s0><s5>10</s5></fC03><fC03 i1="10" i2="X" l="FRE"><s0>Antigène</s0><s5>11</s5></fC03><fC03 i1="10" i2="X" l="ENG"><s0>Antigen</s0><s5>11</s5></fC03><fC03 i1="10" i2="X" l="SPA"><s0>Antígeno</s0><s5>11</s5></fC03><fC03 i1="11" i2="X" l="FRE"><s0>Microélectrode</s0><s5>13</s5></fC03><fC03 i1="11" i2="X" l="ENG"><s0>Microelectrode</s0><s5>13</s5></fC03><fC03 i1="11" i2="X" l="SPA"><s0>Microeléctrodo</s0><s5>13</s5></fC03><fC03 i1="12" i2="X" l="FRE"><s0>Electrode optiquement transparente</s0><s5>33</s5></fC03><fC03 i1="12" i2="X" l="ENG"><s0>Optically transparent electrode</s0><s5>33</s5></fC03><fC03 i1="12" i2="X" l="SPA"><s0>Electrodo ópticamente transparente</s0><s5>33</s5></fC03><fC03 i1="13" i2="X" l="FRE"><s0>1,3-Dithiane-4-valéramide dérivé</s0><s2>NK</s2><s4>INC</s4><s5>76</s5></fC03><fC03 i1="14" i2="X" l="FRE"><s0>1,2-Dithiolane-1,1-dioxyde dérivé</s0><s2>NK</s2><s4>INC</s4><s5>77</s5></fC03><fC03 i1="15" i2="X" l="FRE"><s0>1,2-Dithiolane dérivé</s0><s2>NK</s2><s4>INC</s4><s5>78</s5></fC03><fC07 i1="01" i2="X" l="FRE"><s0>Biodétecteur</s0></fC07><fC07 i1="01" i2="X" l="ENG"><s0>Biosensor</s0></fC07><fC07 i1="01" i2="X" l="SPA"><s0>Biodetector</s0></fC07><fC07 i1="02" i2="X" l="FRE"><s0>Silicium Composé organique</s0><s2>NC</s2><s2>NA</s2><s5>09</s5></fC07><fC07 i1="02" i2="X" l="ENG"><s0>Silicon Organic compounds</s0><s2>NC</s2><s2>NA</s2><s5>09</s5></fC07><fC07 i1="02" i2="X" l="SPA"><s0>Silicio Compuesto orgánico</s0><s2>NC</s2><s2>NA</s2><s5>09</s5></fC07><fN21><s1>175</s1></fN21></pA></standard></inist></record>Pour manipuler ce document sous Unix (Dilib)
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