Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis
Identifieur interne : 000227 ( Ncbi/Curation ); précédent : 000226; suivant : 000228Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis
Auteurs : Najwa Ben Oujji ; Idriss Bakas ; Georges Istamboulié ; Ihya Ait-Ichou ; Elhabib Ait-Addi ; Régis Rouillon ; Thierry NoguerSource :
- Sensors (Basel, Switzerland) [ 1424-8220 ] ; 2012.
Abstract
This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on screen-printed electrodes or microtitration plates and tested using standard solutions and real samples. The developed devices showed good analytical performances with limits of detection much lower than the maximum residue limit tolerated by international regulations, as well as a good reproducibility and stability.
Url:
DOI: 10.3390/s120607893
PubMed: 22969377
PubMed Central: 3436006
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PMC:3436006Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Acetylcholinesterase Immobilized on Magnetic Beads for Pesticides Detection: Application to Olive Oil Analysis</title>
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(N.B.O.);<email>idriss_salame@hotmail.com</email>
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<affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email>
(I.A.-I.);<email>h.aitaddi@esta.ac.ma</email>
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<affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email>
(N.B.O.);<email>idriss_salame@hotmail.com</email>
(I.B.);<email>gistamboulie@yahoo.fr</email>
(G.I.);<email>rouillon@univ-perp.fr</email>
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<affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email>
(I.A.-I.);<email>h.aitaddi@esta.ac.ma</email>
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<author><name sortKey="Istamboulie, Georges" sort="Istamboulie, Georges" uniqKey="Istamboulie G" first="Georges" last="Istamboulié">Georges Istamboulié</name>
<affiliation><nlm:aff id="af1-sensors-12-07893"> IMAGES Laboratory, University of Perpignan, IMAGES EA4218, Building S 52 Av Paul Alduy, 66860 Perpignan Cedex, France; E-Mails:<email>najwa_benoujji@hotmail.com</email>
(N.B.O.);<email>idriss_salame@hotmail.com</email>
(I.B.);<email>gistamboulie@yahoo.fr</email>
(G.I.);<email>rouillon@univ-perp.fr</email>
(R.R.)</nlm:aff>
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<author><name sortKey="Ait Ichou, Ihya" sort="Ait Ichou, Ihya" uniqKey="Ait Ichou I" first="Ihya" last="Ait-Ichou">Ihya Ait-Ichou</name>
<affiliation><nlm:aff id="af2-sensors-12-07893"> AQUAMAR Laboratory, Photocatalysis and Environment Team, Department of Chemistry, Faculty of Science, University Ibn Zohr, BP 8106 Cité Dakhla, Agadir, Morocco; E-Mails:<email>ihya.aitichou@gmail.com</email>
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<front><div type="abstract" xml:lang="en"><p>This work presents the development of bioassays and biosensors for the detection of insecticides widely used in the treatment of olive trees. The systems are based on the covalent immobilisation of acetylcholinesterase on magnetic microbeads using either colorimetry or amperometry as detection technique. The magnetic beads were immobilised on screen-printed electrodes or microtitration plates and tested using standard solutions and real samples. The developed devices showed good analytical performances with limits of detection much lower than the maximum residue limit tolerated by international regulations, as well as a good reproducibility and stability.</p>
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